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Thiocarbamate Inhibition of Fatty Acid Biosynthesis in Isolated Spinach Chloroplasts

Published online by Cambridge University Press:  12 June 2017

R. E. Wilkinson  and
A. E. Smith
R. E. Wilkinson
Affiliation:
Dep. of Agron., Georgia State, Experiment, GA 30212
A. E. Smith
Affiliation:
Dep. of Agron., Georgia State, Experiment, GA 30212
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Abstract

EPTC (S-ethyl dipropylthiocarbamate) (33 μM) and diallate [S-(2,3-dichloroallyl)diisopropylthiocarbamate] (90 μM) inhibited the incorporation of 6 mM acetate-2-14 C (Ac∗) by 80% and 65%, respectively, and the incorporation of 0.5 μM malonate-2-14C (Mal∗) by 32% and 26%, respectively, into the lipids of spinach (Spinacia oleracea L.) chloroplasts. The inhibition of Ac∗ or Mal∗ incorporation into lipids was not observed in the presence of excess Ac∗ or Mal∗, respectively. Incorporation of palmitate-1-14C and oleate-1-14C into chloroplast lipids was inhibited by EPTC and diallate. Mal∗ incorporation into dienoic fatty acids was inhibited by EPTC and diallate. The concentration of EPTC and diallate inhibiting lipid synthesis falls into the physiological range of these herbicides, explains some metabolic effects of these compounds, and fits as the mode of activity of these herbicides.

Type
Research Article
Information
Weed Science , Volume 23 , Issue 2 , March 1975 , pp. 100 - 104
Copyright
Copyright © 1975 by the Weed Science Society of America 

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References

Literature Cited

1. Alred, J.B. and Guy, D.G. 1969. Determinations of coenzyme A and acetyl Co A in tissue extracts. Anal. Biochem. 29:293–299.CrossRefGoogle Scholar
2. Arnon, D.E. 1949. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris . Plant Physiol. 24:1–15.CrossRefGoogle ScholarPubMed
3. Ashton, F.M. 1963. Effect of EPTC on photosynthesis, respiration, and oxidative phosphorylation. Weeds 11:295–297.CrossRefGoogle Scholar
4. Barron, E.J. and Mooney, L.A. 1968. Determination of acylthioesters by gas-liquid chromatography of their sodium borohydride reduction products. Anal. Chem. 40:1742–1744.CrossRefGoogle Scholar
5. Esfahani, M.T., Ioneda, , and Wakil, S.J. 1971. Studies on the control of fatty acid metabolism. III. Incorporation of fatty acids into phospholipids and regulation of fatty acid synthetase of Escherichia coll . J. Biol. Chem. 246:50–56.Google Scholar
6. Gentner, W.A. 1966. The influence of EPTC on external foliage wax development. Weeds 14:27–31.Google Scholar
7. Harris, P. and James, A.T. 1969. The effects of low temperatures on the fatty acid biosynthesis in plants. Biochem. J. 112:325–330.Google Scholar
8. Haslam, J.M., Proudlock, J.W., and Linnane, A.W. 1971. The effects of altered membrane lipid composition on mitochondrial oxidative phosphorylation in Saccharomyces cerevisiae . Bioenergetics 2:351–370.Google Scholar
9. Haslam, J.M., Spithill, T.W., and Linnane, A.W. 1973. The effects of altered membrane lipid composition on cation transport by mitochondria of Saccharomyces cerevisiae . Biochem. J. 134:949–957.CrossRefGoogle ScholarPubMed
10. Hitchcock, C. and Nichols, B.W. 1971. Plant Lipid Biochemistry. Acad. Press., New York, N.Y., 387 pp.Google Scholar
11. Jacobson, B.S., Kannangara, C.G., and Stumpf, P.K. 1973. The elongation of medium chain trienoic acids to ∝-linolenic acid by a spinach chloroplast stroma system. Biochem. Biophys. Res. Comm. 52:1190–1198.Google Scholar
12. Janki, R.M., Aithal, H.N., McMurray, W.C., and Tustanoff, E.R. 1974. The effects of altered membrane-lipid composition on enzyme activities of outer and inner mitochondrial membranes of Saccharomyces cerevisiae . Biochem. Biophys. Res. Comm. 56:1078–1085.CrossRefGoogle ScholarPubMed
13. Kannangara, C.G. and Stumpf, P.K. 1971. The formation of fatty acyl thioesters during 14C-1-acetate incorporation into long chain fatty acids by isolated spinach chloroplasts. Biochem. Biophys. Res. Comm. 44:1544–1551.Google Scholar
14. Mangold, H.K. 1969. Section M. Aliphatic lipids. Pages 363–420, in Stahl, E. ed., Thin-Layer Chromatography. Springer-Verlag, New York.CrossRefGoogle Scholar
15. Mereshimskii, Yu. G., Lapina, T.V., and Ivanischev, V.N. 1970. Oxidation and phosphorylation in seeds under the effect of herbicides and herbicide mixtures. Physiol. Biochem. Cult. Plants (USSR) 2:484–487.Google Scholar
16. Proudlock, J.W., Haslam, J.M., and Linnane, A.W. 1971. The effects of unsaturated fatty acid depletion on the lipid composition and energy metabolism of a fatty acid desaturase mutant of Saccharomyces cerevisiae . Bioenergetics 2:327–349.CrossRefGoogle ScholarPubMed
17. Still, G.G., Davis, D.G., and Zander, G.L. 1970. Plant epicuticular lipids: Alteration by herbicidal carbamates. Plant Physiol. 46:307–314.Google Scholar
18. Vijay, I.K. and Stumpf, P.K. 1971. Fat metabolism in higher plants. XLVI. Nature of the substrate and the product of oleyl Coenzyme A desaturase from Carthamus tinctorius . J. Biol. Chem. 246:2910–2917.Google Scholar
19. Vore, M., Hamilton, J.G., and Lu, A.Y. H. 1974. Organic solvent extraction of liver microsomal lipid. I. The requirement of lipid for 3,4-benzpryene hydroxylase. Biochem. Biophys. Res. Comm. 56:1038–1044.Google Scholar
20. Wilkinson, R.E. 1974. Sicklepod surface wax response to photo-period and diallate. Plant Physiol. 53:269–275.CrossRefGoogle Scholar
21. Wilkinson, R.E. and Hardcastle, W.S. 1969. EPTC effects on sicklepod petiolar fatty acids. Weed Sci. 17:335–338.CrossRefGoogle Scholar
22. Wilkinson, R.E. and Hardcastle, W.S. 1970. EPTC effects on total leaflet fatty acids and hydrocarbons. Weed Sci. 18:125–128.Google Scholar