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Distribution of bipolar input to midget and parasol ganglion cells in marmoset retina

Published online by Cambridge University Press:  18 February 2008

BAHAR ERIKÖZ ,
PATRICIA R. JUSUF ,
KUMIKO A. PERCIVAL  and
ULRIKE GRÜNERT
BAHAR ERIKÖZ
Affiliation:
National Vision Research Institute of Australia, Carlton, Australia Department of Optometry and Vision Sciences, The University of Melbourne, Melbourne, Australia
PATRICIA R. JUSUF
Affiliation:
National Vision Research Institute of Australia, Carlton, Australia Department of Optometry and Vision Sciences, The University of Melbourne, Melbourne, Australia
KUMIKO A. PERCIVAL
Affiliation:
National Vision Research Institute of Australia, Carlton, Australia Department of Optometry and Vision Sciences, The University of Melbourne, Melbourne, Australia
ULRIKE GRÜNERT
Affiliation:
National Vision Research Institute of Australia, Carlton, Australia Department of Optometry and Vision Sciences, The University of Melbourne, Melbourne, Australia
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Abstract

Different types of retinal ganglion cell show differences in their response properties. Here we investigated the question of whether these differences are related to the distribution of the synaptic input to the dendritic tree. We measured the distribution and density of synaptic input to the dendrites of midget and parasol ganglion cells in the retina of a New World monkey, the marmoset, Callithrix jacchus. Ganglion cells were retrogradely labeled by dye injection into parvocellular or magnocellular regions of the lateral geniculate nucleus and subsequently photo-filled. Presumed bipolar cell synapses were identified immunocytochemically using antibodies against the ribbon protein CtBP2 or the GluR4 subunit of the AMPA receptor. For all cells, colocalized immunoreactive puncta were distributed across the entire dendritic tree. The density of the presumed bipolar input to midget ganglion cells was comparable for both synaptic markers, suggesting that the AMPA receptor GluR4 subunit is expressed at all synapses between midget bipolar and midget ganglion cells. Midget ganglion cells had an average of nine colocalized immunoreactive puncta per 100 μm2 dendritic surface, and parasol cells had an average of seven colocalized immunoreactive puncta per 100 μm2 dendritic surface. The densities were comparable in different regions of the dendritic tree and were not influenced by the location of the cells with respect to the fovea. Our findings suggest that the differences in the response characteristics of midget and parasol cells are not due to differences in the density of synaptic input to their dendritic tree.

Keywords

Primate retinaGanglion cellsSynaptic circuitryMagnocellular pathwayParvocellular pathway
Type
Research Article
Information
Visual Neuroscience , Volume 25 , Issue 1 , January 2008 , pp. 67 - 76
Copyright
© 2008 Cambridge University Press

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