Hostname: page-component-cd9895bd7-jkksz Total loading time: 0 Render date: 2024-12-18T15:24:29.089Z Has data issue: false hasContentIssue false
  • English
  • Français

[3H]nicotine binding sites are associated with mammalian optic nerve terminals

Published online by Cambridge University Press:  02 June 2009

Glen T. Prusky  and
Max S. Cynader
Glen T. Prusky
Affiliation:
Departments of Psychology, Dalhousie University, Halifax, Nova Scotia
Max S. Cynader
Affiliation:
Departments of Psychology, Dalhousie University, Halifax, Nova Scotia Physiology and Biophysics, Dalhousie University, Halifax, Nova Scotia
Get access Rights & Permissions [Opens in a new window]

Abstract

The autoradiographic distribution of [3H]nicotine binding sites was examined in the superior colliculus in normal rats and cats, and in animals in which one or both eyes were removed. [3H]Nicotine binding sites in normal animals were densely concentrated in the superficial layers of the colliculus corresponding to the zone of termination of optic nerve fibers. Following bilateral enucleation, [3H]nicotine binding in the superficial collicular layers was drastically reduced. Unilateral enucleation markedly reduced [3H]nicotine binding sites in the colliculus contralateral to the removed eye, with little effect on the ipsilateral colliculus. These results provide further evidence that nicotinic acetylcholine receptors have a presynaptic location on optic tract terminals and may therefore modulate retinotectal transmission in both the rat and cat visual system.

Keywords

Nicotinic receptorAcetylcholineReceptor autoradiographySuperior colliculusRetinal afferentsVision
Type
Short Communications
Information
Visual Neuroscience , Volume 1 , Issue 2 , March 1988 , pp. 245 - 248
Copyright
Copyright © Cambridge University Press 1988

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Boulter, J., Evans, K., Goldman, D., Martin, G., Treco, D., Heinemann, S. & Patrick, J. (1986). Isolation of cDNA clone coding for a possible neuronal nicotinic acetylcholine receptor alpha-Subunit. Nature 319, 368–374.CrossRefGoogle Scholar
Chiappinelli, V.A. (1983). Kappa-bungarotoxin: a probe for the neuronal nicotinic receptor in the avian cilliary ganglion. Brain Research 277, 9–21.CrossRefGoogle Scholar
Clarke, P.B.S., Pert, C.B. & Pert, A. (1984). Autoradiographic distribution of nicotine receptors in the rat brain. Brain Research 323, 390–395.CrossRefGoogle ScholarPubMed
Clarke, P.B.S., Schwartz, R.D., Paul, S.M., Pert, C.B. & Pert, A. (1985). Nicotinic binding in rat brain: autoradiographic comparison of [3H]acetylcholine, [3H]nicotine, and [125I]-alpha-bungaro-toxin. Journal of Neuroscience 5, 1307–1315.CrossRefGoogle Scholar
Egan, T.M. & North, R.A. (1986). Actions of acetylcholine and nicotine on rat locus coeruleus neurons in vitro. Neuroscience 19, 565–571.CrossRefGoogle ScholarPubMed
Francis, A. & Schechter, N. (1980). Putative cholinergic receptor activity in the deafferented rat superior colliculus. Brain Research 183, 224–228.CrossRefGoogle ScholarPubMed
Geary, W.A. & Wooten, G.F. (1985). Regional tritium quenching in quantitative autoradiography of the central nervous system. Brain Research 336, 334–336.CrossRefGoogle ScholarPubMed
Goodman, R.R., Kuhar, M.J., Hester, L. & Snyder, S.H. (1983). Adenosine receptors: autoradiographic evidence for their location on axon terminals of excitatory neurons. Science 220, 967–968.CrossRefGoogle ScholarPubMed
Graybeil, A.M. (1975). Anatomical organization of retinotectal afferents in the cat: an autoradiographic study. Brain Research 96, 1–23.CrossRefGoogle Scholar
Halvorsen, S.W. & Berg, D.K. (1986). Identification of a nicotinic acetylcholine receptor on neurons using an alpha-neurotoxin that blocks receptor function. Neuroscience 6, 3405–3412.CrossRefGoogle ScholarPubMed
Hayhow, W.R., Sefton, A. & Webb, C. (1962). Primary optic centers of the rat in relation to the terminal distribution of the crossed and uncrossed optic nerve terminals. Journal of Comparative Neurology 118, 295–321.CrossRefGoogle Scholar
Henley, J.M., Lindstrom, J.M. & Oswald, R.E. (1986). Acetylcholine receptor synthesis in retina and transport to optic tectum in goldfish. Science 232, 1627–1629.CrossRefGoogle ScholarPubMed
London, E.D., Waller, S.B. & Wamsley, J.K. (1985). Autoradiographic localization of [3H]nicotine binding sites in the rat brain. Neuroscience Letters 53, 179–184.CrossRefGoogle ScholarPubMed
Lund, R.D. (1969). Synaptic patterns of the superficial layers of the superior colliculus of the rat. Journal of Comparative Neurology 135, 179–208.CrossRefGoogle ScholarPubMed
Masland, R.H. (1980). Acetylcholine in the retina. Neurochemistry 1, 501–518.CrossRefGoogle Scholar
Nelson, S.R., Doull, J., Tockman, B.A., Cristiano, P.J. & Samson, F.E. (1978). Regional brain metabolism changes induced by acetylcholinesterase inhibitors. Brain Research 157, 186–190.CrossRefGoogle ScholarPubMed
Noell, W.K. & Lasansky, A. (1959). Effects of electrophoretically applied drugs and electrical currents on the ganglion cell of the retina. Federation Proceedings 18, 115.Google Scholar
Pazdernik, T.L., Cross, R.S., Mewes, K., Samson, F. & Nelson, S.R. (1982). Superior colliculus activation by retinal nicotinic ganglion cells: a 2-deoxyglucose study. Brain Research 243, 197–200.CrossRefGoogle Scholar
Prusky, G.T., Shaw, C. & Cynader, M.S. (in press). The distribution and ontogenesis of [3H]nicotine binding sites in cat visual cortex. Developmental Brain Research.Google Scholar
Schwartz, R.D. (1986). Autoradiographic distribution of high-affinity muscarinic and nicotinic cholinergic receptors labeled with [3H]acetylcholine in rat brain. Life Science 38, 2111–2119.CrossRefGoogle ScholarPubMed
Segu, L., Abdelkefi, J., Dusticier, G. & Lanoir, J. (1986). High-affinity serotonin binding sites: evidence for their location on retinal afferents in the rat superior colliculus. Brain Research 384, 205–217.CrossRefGoogle ScholarPubMed
Swanson, L.W., Simmons, D.M., Whiting, P.J. & Lindstrom, J. (1987). Immunohistochemical localization of neuronal nicotinic receptors in the rodent central nervous system. Journal of Neuroscience 7, 3334–3342.CrossRefGoogle ScholarPubMed
Whiting, P.J., Schoepfer, R., Swanson, L.W., Simmons, D.M. & Lindstrom, J.M. (1987). Functional acetylcholine receptor in PC12 cells reacts with a monoclonal antibody to brain nicotinic receptors. Nature 327, 515–518.CrossRefGoogle ScholarPubMed