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‘Dehydrin-like’ proteins and desiccation tolerance in seeds

Published online by Cambridge University Press:  19 September 2008

O. H. Gee ,
R. J. Probert  and
S. A. Coomber
O. H. Gee
Affiliation:
Division of Life Sciences, King's College London, Campden Hill Road, London W8 7AH, UK Jodrell Laborataory, Royal Botanic Gardens, Kew, Wakehurst Place, Ardingly, West Sussex, RH17 6TN, UK
R. J. Probert*
Affiliation:
Jodrell Laborataory, Royal Botanic Gardens, Kew, Wakehurst Place, Ardingly, West Sussex, RH17 6TN, UK
S. A. Coomber
Affiliation:
Division of Life Sciences, King's College London, Campden Hill Road, London W8 7AH, UK
*
* Correspondence
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Abstract

The relationship between tolerance of seeds to extreme desiccation and the presence of ‘dehydrinlike’ proteins was investigated in groups of related taxa from the unrelated plant families Aceraceae and Gramineae. Dehydrin-like proteins were identified by Western blot analysis using an antibody raised against a synthetic oligopeptide representing the 23-amino acid consensus sequence common to all group 2 late-embryogenesis-abundant (LEA) proteins.

Evidence is presented that seeds of Acer pseudoplatanus and A. saccharinum are desiccation intolerant (recalcitrant) whereas seeds of A. platanoides and A. rubrum are desiccation tolerant (orthodox). Despite these differences, dehydrinlike proteins at 60 and 20 kDa were detected in all four species.

Dehydrins at 20 kDa were also detected in seed samples of two aquatic grasses, Porteresia coarctata and Oryza sativa from the tribe Oryzeae, despite seeds of the former rapidly losing viability on drying, whereas O. sativa is one of the best-known examples of desiccation-tolerant seeds. In O. sativa, there was a correlation between contents of dehydrins detected and the proportion of individuals capable of withstanding extreme drying. However, the possibility of a causal link between these parameters is equivocal. Dehydrin-like proteins were also detected in desiccation-sensitive seeds of Zizania palustris, Z. latifolia and Z. texana and desiccation-intolerant seeds of Spartina anglica, all from the Gramineae.

The presence of group 2 LEAs is clearly not diagnostic of desiccation tolerance in seeds. However, a more direct correlation with the expression of other groups of LEAs cannot be discounted.

Keywords

dehydrinsLEAsdesiccation toleranceseedsAceraceaeGramineae
Type
Short Communication
Information
Seed Science Research , Volume 4 , Issue 2 , June 1994 , pp. 135 - 141
Copyright
Copyright © Cambridge University Press 1994

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References

Aldridge, C.D. (1991) Physiological studies on desiccation intolerance in propagules of aquatic grasses. PhD thesis, Reading University, UK.Google Scholar
Aldridge, C.D. and Probert, R.J. (1993) Seed development, the accumulation of abscisic acid and desiccation tolerance in the aquatic grasses Porteresia coarctata (Roxb.) Tateoka and Oryza sativa L. Seed Science Research 3, 97–103.CrossRefGoogle Scholar
Baker, J.C., Steele, C. and Dure, L. III. (1988) Sequence and characterisation of 6 LEA proteins and their genes from cotton. Plant Molecular Biology 12, 475–486.Google Scholar
Blackman, S.A., Wettlaufer, S.H., Obendorf, R.L. and Leopold, A. C. (1991) Maturation proteins associated with desiccation tolerance in soybean. Plant Physiology 96, 868–874.CrossRefGoogle ScholarPubMed
Bostok, R.M. and Quatrano, R.S. (1992) Regulation of Em gene expression in rice. Plant Physiology 98, 1356–1363.CrossRefGoogle Scholar
Bradford, K.J. and Chandler, P.M. (1992) Expression of ‘dehydrin-like’ proteins in embryos of Zizania palustris and Oryza sativa during dehydration. Plant Physiology 99, 488–494.CrossRefGoogle ScholarPubMed
Bradford, M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principal of protein dye binding. Analytical Biochemistry 72, 248–254.CrossRefGoogle Scholar
Close, T.J., Kortt, A.A. and Chandler, P.M. (1989) A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn. Plant Molecular Biology 11, 277–291.Google Scholar
Dickie, J.B., Ellis, R.H., Kraak, H.L., Ryder, K. and Tompsett, P.B. (1990) Temperature and seed storage longevity. Annals of Botany 65, 197–204.CrossRefGoogle Scholar
Dickie, J.B., May, K., Morris, S.V.A. and Titley, S.E. (1991) The effects of desiccation on seed survival in Acer platanoides L. and Acer pseudoplatanus L. Seed Science Research 1, 149–162.CrossRefGoogle Scholar
Dure, L. III, Crouch, M., Harada, J., Ho, T.H.D., Mundy, J., Quatrano, R.S., Thomas, T. and Sung, Z.R. (1989) Common amino acid sequence domains among LEA proteins of higher plants. Plant Molecular Biology 12, 475–486.CrossRefGoogle ScholarPubMed
Ellis, R.H. and Roberts, E.H. (1980) Improved equations for the prediction of seed longevity. Annals of Botany 45, 13–30.CrossRefGoogle Scholar
Ellis, R.H., Hong, T.D., Roberts, E.H. and Tao, K.L. (1990) Low moisture content limits to relations between seed longevity and moisture. Annals of Botany 65, 493–504.CrossRefGoogle Scholar
Galau, G.A., Hughes, D.W. and Dure, L. III. (1986) Abscisic acid and water-stress induce the expression of a novel rice gene. EMBO Journal 7, 2279–2286.Google Scholar
Galau, G.A., Bijaisoradat, N. and Hughes, D.W. (1987) Accumulation kinetics of cotton late embryogenesis abundant mRNAs and storage protein mRNAs: coordinate regulation during embryogenesis and the role of abscisic acid. Developmental Biology 123, 198–212.CrossRefGoogle ScholarPubMed
Hong, T.D. and Ellis, R.H. (1992) Development of desiccation tolerance in Norway maple (Acer platanoides L.) seeds during maturation drying. Seed Science Research 2, 169–172.CrossRefGoogle Scholar
Hughes, D.W. and Galau, G.A. (1991) Developmental and environmental induction of Lea and LeaA mRNAs and the post-abscission program during embryo culture. Plant Cell 3, 605–618.Google Scholar
International Seed Testing Association (1985) International rules for seed testing. Seed Science and Technology 13, 299–355.Google Scholar
Kermode, A.R. (1990) Regulatory mechanisms involved in the transition from seed development to germination. Critical Reviews in Plant Science 9, 155–195.CrossRefGoogle Scholar
King, M.W. and Roberts, E.H. (1980) A strategy for future research into the storage of recalcitrant seeds. pp 90–110 in Chin, H.F. and Roberts, E.H. (Eds) Recalcitrant crop seeds. Kuala Lumpur, Tropical Press Sdn. Bhd.Google Scholar
Kovach, D.A. and Bradford, K.J. (1991) Imbibitional damage and desiccation tolerance of wild rice (Zizania palustris) seeds. Journal of Experimental Botany 43, 747–757.CrossRefGoogle Scholar
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.CrossRefGoogle ScholarPubMed
Mundy, J. and Chua, N.-H. (1988) Abscisic acid and water-stress induce the expression of a novel rice gene. EMBO Journal 7, 2279–2286.CrossRefGoogle ScholarPubMed
Probert, R.J. and Longley, P.L. (1989) Recalcitrant seed storage physiology in three aquatic grasses (Zizania palustris, Spartina anglica and Porteresia coarctata). Annals of Botany 63, 53–63.CrossRefGoogle Scholar
Ried, J.L. and Walker-Simmons, M.K. (1993) Group 3 late embryogenesis abundant proteins in desiccationtolerant seedlings of wheat (Triticum aestivum L.). Plant Physiology 102, 125–131.CrossRefGoogle ScholarPubMed
Roberts, E.H. (1960) The viability of cereal seed in relation to temperature and moisture content. Annals of Botany 24, 12–31.CrossRefGoogle Scholar
Roberts, E.H. (1961) The viability of rice seed in relation to temperature, moisture content and gaseous environment. Annals of Botany 25, 381–390.CrossRefGoogle Scholar
Roberts, E.H. (1973) Predicting the storage life of seeds. Seed Science and Technology 1, 499–514.Google Scholar
Roberts, E.H., King, M.W. and Ellis, R.H. (1984) Recalcitrant seeds: their recognition and storage, pp 38–52 in Holden, J.H.W. and Williams, J.T. (Eds) Crop genetic resources: conservation and evaluation. George Allen & Unwin, London.Google Scholar
Robertson, M. and Chandler, P.M. (1992) Pea dehydrins: identification characterisation and expression. Plant Molecular Biology 19, 1031–1044.CrossRefGoogle Scholar
Skriver, K. and Mundy, J. (1990) Gene expression in response to abscisic acid and osmotic stress. Plant Cell 2, 503–512.Google ScholarPubMed
Tompsett, P.B. (1984) Desiccation studies in relation to the storage of Araucaria seed. Annals of Applied Biology 105, 581–586.CrossRefGoogle Scholar