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Protease activities and elongation growth of excised cotton seed axes during the first 24 hours of imbibition

Published online by Cambridge University Press:  19 September 2008

Eugene L. Vigil  and
Tung K. Fang
Eugene L. Vigil*
Affiliation:
Climate Stress Laboratory, USDA/ARS, B-046A, BARC-West, Beltsville, MD 20705USA
Tung K. Fang
Affiliation:
Climate Stress Laboratory, USDA/ARS, B-046A, BARC-West, Beltsville, MD 20705USA
*
*Correspondence
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Abstract

Excised axes from cotton (Gossypium hirsutum L. cv. M-8, a double haploid) were imbibed in vitro for 3,6,9,12,18 and 24 h. Data for length, fresh and dry weight and percentage water showed that excised axes undergo a triphasic pattern of growth: an initial burst, a short lag phase and then protracted rapid growth. Analysis of protein content with SDS-PAGE, and of activities of amino-, carboxy- and endopeptidases provided data indicating a direct correlation between major axis elongation growth between 12 and 24 h and enzymatic breakdown of storage proteins (especially those of 48 kDa) by carboxy- and endopeptidase. Data for axes imbibed at 30°C and 0–5°C for 12, 18 and 24 h indicated that a reduction in length and percentage moisture in the cold occurred in parallel with reductions in carboxy- and endopeptidase activities but not in ami-nopeptidase activity. The tentative conclusion is that carboxy- and endopeptidase are probably synthesized de novo in the axis during imbibition and are important in initial breakdown of storage proteins for axial elongation growth.

Keywords

Cottonaxesstorage proteinpeptidasesin vitro germinationSDS-PAGE
Type
Physiology and Biochemistry
Information
Seed Science Research , Volume 5 , Issue 4 , December 1995 , pp. 201 - 207
Copyright
Copyright © Cambridge University Press 1995

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