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Culturable endophytic bacteria from Phelipanche ramosa (Orobanchaceae) seeds

Published online by Cambridge University Press:  17 November 2020

Katarzyna Durlik Open the ORCID record for Katarzyna Durlik [Opens in a new window] ,
Paulina Żarnowiec ,
Renata Piwowarczyk  and
Wiesław Kaca
Katarzyna Durlik*
Affiliation:
Department of Microbiology and Parasitology, Institute of Biology, Jan Kochanowski University, Uniwersytecka 7, 25-406Kielce, Poland
Paulina Żarnowiec
Affiliation:
Department of Microbiology and Parasitology, Institute of Biology, Jan Kochanowski University, Uniwersytecka 7, 25-406Kielce, Poland
Renata Piwowarczyk
Affiliation:
Department of Microbiology and Parasitology, Institute of Biology, Jan Kochanowski University, Uniwersytecka 7, 25-406Kielce, Poland
Wiesław Kaca
Affiliation:
Department of Microbiology and Parasitology, Institute of Biology, Jan Kochanowski University, Uniwersytecka 7, 25-406Kielce, Poland
*
Author for Correspondence: Katarzyna Durlik, E-mail: [email protected]
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Abstract

Endophytic microbiomes play a beneficial role in the development and protection of plants. However, seed-borne endophytic bacteria have not yet been fully explored. Investigation of parasitic plants, whose existence depends on yet poorly understood and complicated relationships with microorganisms and hosts, is particularly crucial. Endophytic bacteria promote seed conservation and facilitate seed germination in soil. Several root holoparasites from the Orobanchaceae family are the most aggressive broomrape species, often causing serious yield losses in important crops. Parasitic plants are characterized by the production of a large number of some of the smallest seeds in the world's flora, allowing them to stay viable in the soil for several dozen years. This study's aim was to isolate and characterize the seed endophyte and surface bacteria of the most aggressive and widespread broomrape weed, Phelipanche ramosa. We isolated two endophytic bacteria from within the seeds which are closely related to Brevibacterium frigoritolerans and Bacillus simplex described as soil bacteria, highly resistant to environmental conditions, and as plant growth-promoting rhizobacteria. Moreover, we isolated three strains from the surface of non-sterile seeds; all three isolates were related to the Bacillus cereus group.

Keywords

BacillusBrevibacterium frigoritoleransbroomrapeholoparasitesPhelipanche ramosaseed endophytes
Type
Research Paper
Information
Seed Science Research , Volume 31 , Issue 1 , March 2021 , pp. 69 - 75
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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