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Naf1p is a box H/ACA snoRNP assembly factor

Published online by Cambridge University Press:  16 January 2003

ALESSANDRO FATICA ,
MENSUR DLAKIĆ  and
DAVID TOLLERVEY
ALESSANDRO FATICA
Affiliation:
Wellcome Trust Centre for Cell Biology, Swann Building, King's Buildings, University of Edinburgh, Edinburgh EH9 3JR, UK
MENSUR DLAKIĆ
Affiliation:
Department of Biological Chemistry, The University of Michigan Medical School, Ann Arbor, MI 48109-0606, USA
DAVID TOLLERVEY
Affiliation:
Wellcome Trust Centre for Cell Biology, Swann Building, King's Buildings, University of Edinburgh, Edinburgh EH9 3JR, UK
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Abstract

Box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs) contain four essential proteins, Cbf5p, Gar1p, Nhp2p, and Nop10p, each of which, with the exception of Gar1p, is required for box H/ACA snoRNA accumulation. Database searches identified a novel essential protein, which we termed Naf1p, with a region of homology to the RNA-binding domain of Gar1p and other features in common with hnRNP-like proteins. Naf1p is localized to the nucleus and is not a stable component of the H/ACA snoRNPs, but it is required for the accumulation of all box H/ACA snoRNAs. This requirement is not at the level of snoRNA transcription initiation or termination. Naf1p shows in vitro RNA-binding activity and also binds directly to Cbf5p and Nhp2p. Naf1p was shown to bind to the CTD in vivo in a two-hybrid assay, and the phosphorylated CTD, but not the nonphosphorylated CTD, was shown to precipitate tagged Naf1p from a cell lysate. We propose that Naf1p is recruited to the CTD of RNA polymerase II and binds to nascent box H/ACA snoRNAs promoting snoRNP assembly.

Keywords

ACA snoRNPsbox HsnoRNP assembly
Type
Research Article
Information
RNA , Volume 8 , Issue 12 , December 2002 , pp. 1502 - 1514
Copyright
2002 RNA Society

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