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Advances in superresolution optical fluctuation imaging (SOFI)

Published online by Cambridge University Press:  14 May 2013

Thomas Dertinger ,
Alessia Pallaoro ,
Gary Braun ,
Sonny Ly ,
Ted A. Laurence  and
Shimon Weiss
Thomas Dertinger*
Affiliation:
Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095, USA
Alessia Pallaoro
Affiliation:
Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA 93106, USA
Gary Braun
Affiliation:
Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA 93106, USA
Sonny Ly
Affiliation:
Lawrence Livermore National Laboratory, Livermore, CA 94550, USA
Ted A. Laurence
Affiliation:
Lawrence Livermore National Laboratory, Livermore, CA 94550, USA
Shimon Weiss*
Affiliation:
Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095, USA Department of Physiology University of California Los Angeles, UCLA, Los Angeles, USA California NanoSystems Institute University of California Los Angeles, UCLA, Los Angeles, USA
*
*Authors for Correspondence: Shimon Weiss, 607 Charles E. Young Drive East, Los Angeles, CA 90095-1569, USA. Email: [email protected] and Thomas Dertinger, Dresdener Str. 14, 10999 Berlin, Germany. Email: [email protected]
*Authors for Correspondence: Shimon Weiss, 607 Charles E. Young Drive East, Los Angeles, CA 90095-1569, USA. Email: [email protected] and Thomas Dertinger, Dresdener Str. 14, 10999 Berlin, Germany. Email: [email protected]
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Abstract

We review the concept of superresolution optical fluctuation imaging (SOFI), discuss its attributes and trade-offs (in comparison with other superresolution methods), and present superresolved images taken on samples stained with quantum dots, organic dyes, and plasmonic metal nanoparticles. We also discuss the prospects of SOFI for live cell superresolution imaging and for imaging with other (non-fluorescent) contrasts.

Type
Review Article
Information
Quarterly Reviews of Biophysics , Volume 46 , Issue 2 , May 2013 , pp. 210 - 221
Copyright
Copyright © Cambridge University Press 2013 

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