Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-27T20:48:36.012Z Has data issue: false hasContentIssue false
  • English
  • Français

Towards a novel marker of insulin resistance in obesity: S100A4 in girls along the puberty. The longitudinal study “PUBMEP”

Published online by Cambridge University Press:  10 June 2020

Augusto Anguita-Ruiz ,
Andrea Méndez-Gutierrez ,
Azahara I. Ruperez ,
Rosaura Leis ,
Gloria Bueno ,
Mercedes Gil-Campos ,
Angel Gil  and
Concepción M. Aguilera
Augusto Anguita-Ruiz
Affiliation:
Department of Biochemistry and Molecular Biology II, Institute of Nutrition and Food Technology “José Mataix”, Center of Biomedical Research, University of Granada, Granada, Spain CIBEROBN (Physiopathology of Obesity and Nutrition Network CB12/03/30038), Instituto de Salud Carlos III (ISCIII), Madrid, Spain
Andrea Méndez-Gutierrez
Affiliation:
Department of Biochemistry and Molecular Biology II, Institute of Nutrition and Food Technology “José Mataix”, Center of Biomedical Research, University of Granada, Granada, Spain
Azahara I. Ruperez
Affiliation:
CIBEROBN (Physiopathology of Obesity and Nutrition Network CB12/03/30038), Instituto de Salud Carlos III (ISCIII), Madrid, Spain Growth, Exercise, NUtrition and Development (GENUD) Research Group, University of Zaragoza, Zaragoza, Spain
Rosaura Leis
Affiliation:
CIBEROBN (Physiopathology of Obesity and Nutrition Network CB12/03/30038), Instituto de Salud Carlos III (ISCIII), Madrid, Spain Unit of Investigation in Nutrition, Growth and Human Development of Galicia, Pediatric Department (University of Santiago de Compsotela, Santiago de Compostela, Spain
Gloria Bueno
Affiliation:
CIBEROBN (Physiopathology of Obesity and Nutrition Network CB12/03/30038), Instituto de Salud Carlos III (ISCIII), Madrid, Spain Growth, Exercise, NUtrition and Development (GENUD) Research Group, University of Zaragoza, Zaragoza, Spain
Mercedes Gil-Campos
Affiliation:
CIBEROBN (Physiopathology of Obesity and Nutrition Network CB12/03/30038), Instituto de Salud Carlos III (ISCIII), Madrid, Spain Department of Paediatrics, Reina Sofia University Hospital, Institute Maimónides of Biomedicine Investigation of Córdoba (IMIBIC), University of Córdoba, Córdoba, Spain
Angel Gil
Affiliation:
Department of Biochemistry and Molecular Biology II, Institute of Nutrition and Food Technology “José Mataix”, Center of Biomedical Research, University of Granada, Granada, Spain CIBEROBN (Physiopathology of Obesity and Nutrition Network CB12/03/30038), Instituto de Salud Carlos III (ISCIII), Madrid, Spain
Concepción M. Aguilera
Affiliation:
Department of Biochemistry and Molecular Biology II, Institute of Nutrition and Food Technology “José Mataix”, Center of Biomedical Research, University of Granada, Granada, Spain CIBEROBN (Physiopathology of Obesity and Nutrition Network CB12/03/30038), Instituto de Salud Carlos III (ISCIII), Madrid, Spain
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.
Introduction:

Insulin resistance (IR) is the major driver for the development of obesity-associated metabolic and cardiovascular complications. It is well known that IR increase physiologically during puberty; hence, pubertal maturation might favour this metabolic risk in obese children. Recently, a study carried out in adult women with obesity has identified a new adipokine, known as S100A4, strongly associated with IR and inflammation in adipose tissue. On the contrary, little is known about the implication of S100A4 in the development of such metabolic disturbances during the onset and course of pubertal development.

Materials and methods:

A longitudinal study was conducted on 53 Spanish girls distributed in six experimental conditions according to their obesity and IR status (before (T0) and after (T1) the onset of puberty). Anthropometric and biochemical parameters were evaluated in all samples and time points. Classification of pubertal stage was made according to the Tanner scale. S100A4 protein levels were quantified by ELISA CSB-EL02032HU in plasma samples (Cusabio Biotech, Wuhan, China). The statistical analysis of the results was carried out with the “nlme” package in R v3.4.4, using a mixed-effects linear model with random intercept and slope.

Results:

At a significance level of alpha = 0.05, a linear mixed-effects model reported a significant association (P = 0.03) between the interaction term “time*experimental group” and S100A4 levels. Post-hoc pairwise comparisons between experimental groups revealed a strong association between a worsening/improvement of the IR status and the increase/decrease of S100A4 levels (yielding significant results for 5 of the 15 comparisons (P = 0.008, P = 0.04, P = 0.02, P = 0.04 and P = 0.02)). Furthermore, a multiple linear regression model reported a positive correlation between the increase in S100A4 levels and the increase in HOMA values during the course of puberty (B = 6.03, SE = 2.66 and P = 0.028).

Discussion:

The S100A4 protein is strongly associated with the development of IR in girls with childhood obesity and this association is accentuated during pubertal development. Increase in S100A4 levels could be one of the molecular mechanisms by which pubertal maturation favour an increased metabolic risk in children with obesity.

Type
Abstract
Information
Proceedings of the Nutrition Society , Volume 79 , Issue OCE2: 13th European Nutrition Conference, FENS 2019, 15–18 October 2019, Malnutrition in an Obese World: European Perspectives , 2020 , E646
Check for updates
Copyright
Copyright © The Authors 2020