Wheat is a major source of dietary fibre (DF) in the UK diet, with bread alone providing 20% of the total daily intake in adults(Reference Steer, Thane, Stephen and Jebb1). Furthermore, although white bread contains less DF (2·5–4·5%) than wholemeal (10–14%), the high consumption (about 65% of total bread products) means that it alone contributes 11% of the total intake of DF(Reference Steer, Thane, Stephen and Jebb1). The major DF components in white flour and bread are cell wall polysaccharides, principally arabinoxylan (AX) and β-glucan which account for about 70% and 20% of the total, respectively.
Analyses of 150 wheat cultivars grown on the same site showed that the total content of AX in white flour ranged from 1·35% to 2·75% dry wt, and of soluble AX from 0·30% to 1·40%(Reference Gebruers, Dornez and Bedő2). The proportion of soluble AX was generally about 25% of the total. However, the Chinese cultivar Yumai 34 was shown to contain the highest contents of both total and soluble AX, and is now being exploited by wheat breeders as a source of these traits.
We have now analysed wheat lines of wider genetic diversity, including a core set of lines from the Watkins collection of land races which were collected from 32 European, Asian and North African Countries in the 1920s and 1930s(Reference Miller, Ambrose, Reader, Caligari and Brandham3). Preliminary analyses showed that the range of content of total AX in wholemeal was wider than in the 150 cultivars, with up to 35% soluble AX (Figure 1). This high solubility may be related to differences in the fine structure of AX, particularly the ratio of xylose residues which are substituted with single and two arabinose residues: this is being compared by fingerprinting the patterns of oligosaccharides released after digestion with endoxylanase.
Fig. 1. Content of water-extractable AX (WE-AX) (expressed as % age of total AX) in wholemeal flours of Watkins lines. AX was determined as pentose units(Reference Douglas4).
The lines will be used to improve the DF content and composition of bread wheat. They will also allow us to explore the relationship between the structure and properties of AX and its contribution to human health, including viscosity and prebioitic effects in the colon.
Research at Rothamsted is funded by the Biotechnology and Biological Sciences Research Council (BBSRC) of the UK.
