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Comparison of commercial DNA extraction kits and sample preparation for extraction of bacterial DNA from different fractions of whole rumen fluid

Published online by Cambridge University Press:  23 November 2017

A Dorel ,
N D Scollan ,
S A Huws ,
D R Yáñez Ruiz  and
C J Newbold
A Dorel*
Affiliation:
Institute of Grassland and Environmental Research, Aberystwyth, Ceredigion, United Kingdom
N D Scollan
Affiliation:
Institute of Grassland and Environmental Research, Aberystwyth, Ceredigion, United Kingdom
S A Huws
Affiliation:
Institute of Grassland and Environmental Research, Aberystwyth, Ceredigion, United Kingdom
D R Yáñez Ruiz
Affiliation:
Institute of Rural Science, University of Wales, Aberystwyth, Aberystwyth, Ceredigion, United Kingdom
C J Newbold
Affiliation:
Institute of Rural Science, University of Wales, Aberystwyth, Aberystwyth, Ceredigion, United Kingdom
*
Email: [email protected]
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Extract

Previous work has shown that the biohydrogenation of fatty acids is different in solid and liquid fractions prepared from rumen fluid (Dorel et al. , 2005). This study aimed to relate this observation to differences in the microbial population in the different fractions. Molecular techniques allow the rapid investigation of microbial numbers and diversity in environmental samples. However, description of the microbial diversity can be affected by the efficiency of DNA extraction from samples as it is known that it is harder to quantitatively extract DNA from some microbes than others (Clement and Kitts, 2000). The aim of this study was to investigate different protocols for preparing samples and extracting DNA from different fractions of rumen fluid, in terms of both the numbers and diversity of bacteria that could be recovered.

Type
Poster presentations
Information
Proceedings of the British Society of Animal Science , Volume 2006 , March 2006 , pp. 134
Copyright
Copyright © 2016 The American Society of International Law

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References

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