Since in ruminants urinary excretion of purine derivatives is related to duodenal absorption of purines (Fujihara et al.1987;Giesecke et al.1984), it may be a valuable index to identify nutritive constraints limiting rumen fermentation and microbial synthesis.
In the present experiment, the response in the urinary excretion of purine derivatives to urea supplementation of a N deficient diet was studied to test the validity of the above assumption.
Five Rasa Aragonesa ewes rumen cannulated and 38, 9±2, 52 Kg mean live weight, were offered “ad libitum” OHNa treated barley straw (50 g/Kg DM) ground and pelleted.
Five experimental treatments including four levels of urea supplementation(6.5, 13, 19.5 and 26 g/d) plus a negative control were arranged in a 5x5 latin square design,allowing 14 days of adaptation between experimental periods.The urea was given by continuous infusion to the rumen at a flow rate of 0.92 ml/mn during 18 h per day starting after feeding.