Given their strategic position within American society, clergy continue to remain important actors in American politics. This article examines the partisan identifications and electoral behavior of American Protestant clergy in the 2016 presidential election. Although clergy partisanship may be of interest in any election, the 2016 contest, given the milieu of political polarization and the presence of the Trump candidacy, provides an intriguing context for assessing the profession's electoral behavior, particularly among Republican clergy. Based on survey results from over 2,500 clergy drawn from ten Protestant (five mainline and five evangelical) denominations, the study finds that, during the early stages of the 2016 nomination process, only a small percentage of Republican clergy supported Trump and that, despite the high level of political polarization, a sizable segment of Republican clergy resisted partisan pressures and refused to vote for Trump in the general election. The propensity of both independent and Republican clergy to vote for the GOP nominee varied largely with the level of perceived “threats”: to the Christian heritage of the nation, from Islam, and from the process of “globalization.”