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Modified AFLP technique for fingerprinting of Anemone coronaria

Published online by Cambridge University Press:  12 February 2007

J. Fang  and
T. Paz
J. Fang*
Affiliation:
Nanjing Agricultural University, Nanjing, 210095, China
T. Paz
Affiliation:
Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet-Dagan 50 250, Israel
*
*Corresponding author: Department of Botany and Plant Sciences, University of California–Riverside, Riverside, CA 92 521, USA. E-mail: [email protected]
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Abstract

We successfully generated clear amplified fragment length polymorphism (AFLP) profiles of Anemone coronaria using only a single restriction enzyme (EcoR I). Four primer combinations generated 571 bands in 37 anemone accessions with an average 142.75 bands per primer combination. Polymorphism among the 37 samples was very high (98.15%). This high polymorphism showed that the one-enzyme AFLP method is effective for the assessment of genetic diversity as well as cultivar differentiation of anemone. We observed 26 bands which differentiated between fingerprints derived from leaf and corm DNA of eight plant individuals. However, the low percentage of such bands showed that anemone corms can also be used to evaluate anemone germplasm using AFLP markers.

Keywords

AFLPAnemone coronariafingerprintingmodificationpolymorphic bands
Type
Short communication
Information
Plant Genetic Resources , Volume 3 , Issue 1 , April 2005 , pp. 73 - 75
Copyright
Copyright © NIAB 2005

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References

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