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Applicability of chromosome-specific SSR wheat markers for the introgression of Triticum urartu in durum wheat breeding programmes

Published online by Cambridge University Press:  20 April 2011

C. Rodríguez-Suárez ,
M. C. Ramírez ,
A. Martín  and
S. G. Atienza
C. Rodríguez-Suárez*
Affiliation:
Instituto de Agricultura Sostenible, IAS-CSIC, Apdo. 4084, 14080Córdoba, Spain
M. C. Ramírez
Affiliation:
Instituto de Agricultura Sostenible, IAS-CSIC, Apdo. 4084, 14080Córdoba, Spain
A. Martín
Affiliation:
Instituto de Agricultura Sostenible, IAS-CSIC, Apdo. 4084, 14080Córdoba, Spain
S. G. Atienza
Affiliation:
Instituto de Agricultura Sostenible, IAS-CSIC, Apdo. 4084, 14080Córdoba, Spain
*
*Corresponding author. E-mail: [email protected]
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Abstract

Triticum urartu, the A-genome donor of tetraploid and hexaploid wheats, is a potential source of novel alleles for crop improvement. A fertile amphiploid between T. urartu (2n = 2x = 14; AuAu) and durum wheat cv ‘Yavaros’ (Triticum turgidum ssp. durum; 2n = 4x = 28, AABB) was obtained as a first step to making the genetic variability of the wild ancestor available to durum wheat breeding. The amphiploid was backcrossed with ‘Yavaros’ and the offspring from this cross was selfed. A plant from this progeny (founder line) with 28 chromosomes and active x and y subunits of the Glu-A1 locus of T. urartu was selfed, which resulted in the obtaining of 98 pre-introgression lines (pre-ILs). In this work, a set of 78 wheat chromosome-specific microsatellite markers (simple sequence repeats, SSR), uniformly distributed over the A genome, was used for marker-assisted selection of T. urartu in a durum wheat background. A total of 57 SSRs allowed a clear discrimination between T. urartu and ‘Yavaros’. This set of markers was further used for characterizing the pre-ILs, identifying and defining the T. urartu introgressed regions. The applicability of these markers is discussed.

Keywords

introgressions linesmarker assisted selectionsimple sequence repeatsTriticum urartu
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 3 , August 2011 , pp. 439 - 444
Copyright
Copyright © NIAB 2011

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References

Alvarez, JB, Caballero, L, Nadal, S, Ramirez, CM and Martín, A (2009) Development and gluten strength evaluation of introgression lines of Triticum urartu in durum wheat. Cereal Research Communications 37: 243–248.CrossRefGoogle Scholar
Atienza, SG, Ramirez, CM, Hernandez, P and Martin, A (2004) Chromosomal location of genes for carotenoid pigments in Hordeum chilense. Plant Breeding 123: 303–304.CrossRefGoogle Scholar
Atienza, S, Avila, CM and Martín, A (2007a) The development of a PCR-based marker for PSY1 from Hordeum chilense, a candidate gene for carotenoid content accumulation in tritordeum seeds. Australian Journal of Agriculture Research 58: 767–773.CrossRefGoogle Scholar
Atienza, SG, Ballesteros, J, Martin, A and Hornero-Mendez, D (2007b) Genetic variability of carotenoid concentration and degree of esterification among tritordeum ( × Tritordeum Ascherson et Graebner) and durum wheat accessions. Journal of Agricultural and Food Chemistry 55: 4244–4251.CrossRefGoogle ScholarPubMed
Börner, A, Schumann, E, Fürste, A, Cöster, H, Leithold, B, Röder, M and Weber, W (2002) Mapping of quantitative trait loci determining agronomic important characters in hexaploid wheat (Triticum aestivum L.). Theoretical and Applied Genetics 105: 921–936.CrossRefGoogle ScholarPubMed
Brondani, C, Rangel, PHN, Brondani, RPV and Ferreira, ME (2002) QTL mapping and introgression of yield-related traits from Oryza glumaepatula to cultivated rice (Oryza sativa) using microsatellite markers. Theoretical and Applied Genetics 104: 1192–1203.CrossRefGoogle ScholarPubMed
Carmona, S, Caballero, L, Martín, LM and Alvarez, JB (2010) Genetic diversity in khorasan and rivet wheat by assessment of morphological traits and seed storage proteins. Crop and Pasture Sciences 61: 938–944.CrossRefGoogle Scholar
Chapman, V, Miller, TE and Riley, R (1976) Equivalence of the A genome of bread wheat and that of Triticum urartu. Genetics Research 27: 69–76.CrossRefGoogle Scholar
di Pietro, JP, Caillaud, CM, Chaubet, B, Pierre, JS and Trottet, M (1998) Variation in resistance to the grain aphid, Sitobion avenae (Sternorhynca: Aphididae), among diploid wheat genotypes: multivariate analysis of agronomic data. Plant Breeding 117: 407–412.CrossRefGoogle Scholar
Dvořák, J (1976) The relationship between the genome of Triticum urartu and the A and B genomes of Triticum aestivum. Canadian Journal of Genetics and Cytology 18: 371–377.CrossRefGoogle Scholar
Eshed, Y and Zamir, D (1994) A genomic library of Lycopersicon pennellii in L. esculentum: a tool for fine mapping of genes. Euphytica 79: 175–179.CrossRefGoogle Scholar
Fetch, TG Jr, Steffenson, BJ and Nevo, E (2003) Diversity and sources of multiple disease resistance in Hordeum spontaneum. Plant Disease 87: 1439–1448.CrossRefGoogle ScholarPubMed
Huang, XQ, Coster, H, Ganal, MW and Röder, MS (2003) Advanced backcross QTL analysis for the identification of quantitative trait loci alleles from wild relatives of wheat (Triticum aestivum L.). Theoretical and Applied Genetics 106: 1379–1389.CrossRefGoogle ScholarPubMed
Huang, XQ, Kempf, H, Ganal, MW and Röder, MS (2004) Advanced backcross QTL analysis in progenies derived from a cross between a German elite winter wheat variety and a synthetic wheat (Triticum aestivum L.). Theoretical and Applied Genetics 109: 933–943.CrossRefGoogle Scholar
Lawrence, GJ and Shepherd, KW (1981) Inheritance of glutenin protein subunits of wheat. Theoretical and Applied Genetics 60: 333–337.CrossRefGoogle ScholarPubMed
Lee, M (1998) Genome projects and gene pools: new germplasm for plant breeding? Proceedings of the National Academy of Sciences of the United States of America 95: 2001–2004.CrossRefGoogle ScholarPubMed
Martín, MA, Martín, LM and Alvarez, JB (2008) Polymorphisms at the Gli-Au1 and Gli-Au2 loci in wild diploid wheat (Triticum urartu). Euphytica 163: 303–307.CrossRefGoogle Scholar
Matus, I, Corey, A, Filichkin, T, Hayes, PM, Vales, MI, Kling, J, Riera-Lizarazu, O, Sato, K, Powell, W and Waugh, R (2003) Development and characterization of recombinant chromosome substitution lines (RCSLs) using Hordeum vulgare subsp. spontaneum as a source of donor alleles in a Hordeum vulgare subsp. vulgare background. Genome 46: 1010–1023.CrossRefGoogle Scholar
Murray, YHG and Thompson, WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8: 4321–4326.CrossRefGoogle ScholarPubMed
Payne, PI, Law, CN and Mudd, EE (1980) Control by homeologous group I chromosomes of the high molecular weight subunits of glutenin, a major protein of wheat endosperm. Theoretical and Applied Genetics 58: 113–120.CrossRefGoogle Scholar
Pestsova, E, Börner, A and Röder, MS (2006) Development and QTL assessment of Triticum aestivum–Aegilops tauschii introgression lines. Theoretical and Applied Genetics 112: 634–647.CrossRefGoogle ScholarPubMed
Piergiovanni, AR (2009) Estimating gliadin and albumin variation at intra- and interaccession level in USDA oriental wheat (Triticum turgidum L. subsp. turanicum (Jakubz.) (A. Lőve & D. Lőve) collection using capillary zone electrophoresis. Cereal Chemistry 86: 37–43.CrossRefGoogle Scholar
Qiu, YC, Zhou, RH, Kong, XY, Zhang, SS and Jia, JZ (2005) Microsatellite mapping of a Triticum urartu Tum. derived powdery mildew resistance gene transferred to common wheat (Triticum aestivum L.). Theoretical and Applied Genetics 111: 1524–1531.CrossRefGoogle ScholarPubMed
Röder, MS, Korzun, V, Wendehake, K, Plaschke, J, Tixier, MH, Leroy, P and Ganal, MW (1998) A microsatellite map of wheat. Genetics 149: 2007–2023.CrossRefGoogle ScholarPubMed
Rodríguez-Suárez, C, Giménez, MJ and Atienza, SG (2010) Progress and perspectives for carotenoid accumulation in selected Triticeae species. Crop and Pasture Sciences 61: 743–751.CrossRefGoogle Scholar
Somers, DJ, Isaac, P and Edwards, K (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics 109: 1105–1114.CrossRefGoogle ScholarPubMed
Sourdille, P, Singh, S, Cadalen, T, Brown-Guedira, GL, Gay, G, Qi, L, Gill, BS, Dufour, P, Murigneux, A and Bernard, M (2004) Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.). Functional and Integrative Genomics 4: 12–25.CrossRefGoogle ScholarPubMed
Tanksley, SD and McCouch, SR (1997) Seed banks and molecular maps: unlocking genetic potential from the wild. Science 277: 1063–1068.CrossRefGoogle ScholarPubMed
van Ginkel, M and Ogbonnaya, F (2007) Novel genetic diversity from synthetic wheats in breeding cultivars for changing production conditions. Field Crops Research 104: 86–94.CrossRefGoogle Scholar
Warburton, ML, Crossa, J, Franco, J, Kazi, M, Trethowan, R, Rajaram, S, Pfeiffer, W, Zhang, P, Dreisigacker, S and van Ginkel, M (2006) Bringing wild relatives back into the family: recovering genetic diversity in CIMMYT improved wheat germplasm. Euphytica 149: 289–301.CrossRefGoogle Scholar
Young, ND and Tanksley, SD (1989) Restriction fragment length polymorphism maps and the concept of graphical genotypes. Theoretical and Applied Genetics 77: 95–101.CrossRefGoogle ScholarPubMed
Yun, SJ, Gyenis, L, Hayes, PM, Matus, I, Smith, KP, Steffenson, BJ and Muehlbauer, GJ (2005) Quantitative trait loci for multiple disease resistance in wild barley. Crop Sciences 45: 2563–2572.CrossRefGoogle Scholar
Xu, LL, Li, W, Wei, YM and Zheng, YL (2009) Genetic diversity of HMW glutenin subunits in diploid, tetraploid and hexaploid Triticum species. Genetic Resources and Crop Evolution 56: 377–391.CrossRefGoogle Scholar
Zhang, W, Lukaszewski, AJ, Kolmer, J, Soria, MA, Goyal, S and Dubcovsky, J (2005) Molecular characterization of durum and common wheat recombinant lines carrying leaf resistance (Lr19) and yellow pigment (Y) genes from Lophopyrum ponticum. Theoretical and Applied Genetics 111: 573–582.CrossRefGoogle ScholarPubMed
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