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Analysis of high-molecular-weight glutenin subunits in five amphidiploids and their parental diploid species Aegilops umbellulata and Aegilops uniaristata

Published online by Cambridge University Press:  16 June 2014

Shoufen Dai ,
Li Zhao ,
Xiaofei Xue ,
Yanni Jia ,
Dengcai Liu ,
Zongjun Pu ,
Youliang Zheng  and
Zehong Yan
Shoufen Dai
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu611130, Sichuan, People's Republic of China
Li Zhao
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu611130, Sichuan, People's Republic of China
Xiaofei Xue
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu611130, Sichuan, People's Republic of China
Yanni Jia
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu611130, Sichuan, People's Republic of China
Dengcai Liu
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu611130, Sichuan, People's Republic of China Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining810001, People's Republic of China
Zongjun Pu
Affiliation:
Crop Research Institute, Sichuan Academy of Agricultural Science, Chengdu610066, People's Republic of China
Youliang Zheng
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu611130, Sichuan, People's Republic of China
Zehong Yan*
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu611130, Sichuan, People's Republic of China
*
*Corresponding author. E-mail: [email protected]
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Abstract

Amphidiploids serve as a bridge for transferring genes from wild species into wheat. In this study, five amphidiploids with AABBUU and AABBNN genomes were produced by spontaneous chromosome doubling of unreduced triploid F1 gametes from crosses between diploid Aegilops (A. umbellulata accessions CIae 29 and PI 226500, and A. uniaristata accession PI 554419) and tetraploid Triticum turgidum (ssp. durum cultivar Langdon and ssp. dicoccum accessions PI 94 668 and PI 349045) species. The composition of high-molecular-weight glutenin subunits (HMW-GS) in these amphidiploids and in their parental A. umbellulata and A. uniaristata species was analysed. As expected, the amphidiploids from T. turgidum ssp. dicoccum accession PI 944668 or PI 349045 and A. umbellulata accession CIae 29 or PI 226500 and A. uniaristata accession PI 554419 showed the same HMW-GS patterns as those of their Aegilops parents, because HMW-GS genes were all silenced in the T. turgidum ssp. dicoccum parents. The amphidiploids from CIae 29 and Langdon inherited all of the HMW-GS genes from their parents except for the Uy type. Using 10 and 15% sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) and 10% urea/SDS–PAGE, 11 Ux and ten Uy types in 16 combinations were observed in 48 A. umbellulata accessions, and two Nx and two Ny types in two combinations were detected in six A. uniaristata accessions. These novel HMW-GS variants may provide new genetic resources for improving the quality of wheat.

Keywords

Aegilops umbellulataAegilops uniaristataamphidiploidsHMW-GSsynthetic hexaploid speciesTriticum turgidum
Type
Short Communication
Information
Plant Genetic Resources , Volume 13 , Issue 2 , August 2015 , pp. 186 - 189
Copyright
Copyright © NIAB 2014 

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References

Castilho, A, Miller, TE and Heslop-Harrison, JS (1996) Physical mapping of translocation breakpoints in a set of wheat-Aegilops umbellulata recombinant lines using in situ hybridization. Theoretical and Applied Genetics 93: 816–825.CrossRefGoogle Scholar
Castilho, A, Miller, TE and Heslop-Harrison, JS (1997) Analysis of a set of homoeologous group 1 wheat-Aegilops umbellulata recombinant chromosome lines using genetic markers. Theoretical and Applied Genetics 94: 293–297.CrossRefGoogle Scholar
De Bustos, A and Jouve, N (2006) Characterization and phylogenetic analysis of the genes coding for high molecular weight glutenin subunits in three diploid species of Aegilops . International Journal of Plant Science 167: 359–366.CrossRefGoogle Scholar
Hu, XK, Dai, SF, Pu, ZE, Liu, DC, Pu, ZJ, Jiang, JQ, Wei, YM, Wu, BH, Lan, XJ, Zheng, YL and Yan, ZH (2013) Quality of synthetic hexaploid wheat containing null alleles at Glu-A1 and Glu-B1 loci. Journal of Genetics 92: 241–245.CrossRefGoogle ScholarPubMed
Jiang, JM, Friebe, B and Gill, BS (1994) Recent advances in alien gene transfer in wheat. Euphytica 73: 199–212.CrossRefGoogle Scholar
Kilian, B, Mammen, K, Millet, E, Sharma, R, Graner, A, Salamini, F, Hammer, K and Özkan, H (2011) Aegilops . In: Kole, C (ed.) Wild Crop Relatives: Genomic and Breeding Resources: Cereals. Berlin, Heidelberg: Springer-Verlag, pp. 1–76.Google Scholar
Liu, Z, Yan, Z, Wan, Y, Liu, K, Zheng, Y and Wang, D (2003) Analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops species. Theoretical and Applied Genetics 106: 1368–1378.CrossRefGoogle ScholarPubMed
Mujeeb-Kazi, A, Rosas, V and Roldan, S (1996) Conservation of the genetic variation of Triticum tauschii (Coss.) Schmalh. (Aegilops squarrosa auct. non L.) in synthetic hexaploid wheats (Triticum turgidum L. s.lat. × Triticum tauschii; 2n = 6x = 42, AABBDD) and its potential utilization for wheat improvement. Genetic Resources and Crop Evolution 43: 129–134.CrossRefGoogle Scholar
Payne, PI, Law, CN and Mudd, EE (1980) Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theoretical and Applied Genetics 58: 113–120.CrossRefGoogle Scholar
Payne, PI, Holt, LM and Law, CN (1981) Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin: part 1: allelic variation in subunits amongst varieties of wheat (Triticum aestivum). Theoretical and Applied Genetics 60: 229–236.CrossRefGoogle ScholarPubMed
Payne, PI, Holt, LM, Jackson, EA and Law, CN (1984) Wheat storage protein: their genetics and their potential for manipulation by plant breeding. Philosophical Transactions of the Royal Society London B 304: 359–371.Google Scholar
Peña, RJ, Zarco-Hernandez, J and Mujeeb-Kazi, A (1995) Glutenin subunit compositions and bread-making quality characteristics of synthetic hexaploid wheats derived from Triticum turgidum× Triticum tauschii (coss.) Schmal Crosses. Journal of Cereal Science 21: 15–23.CrossRefGoogle Scholar
Pflüger, LA, D'Ovidio, R, Margiotta, B, Peña, R, Mujeeb-Kazi, A and Lafiandra, D (2001) Characterisation of high- and low-molecular weight glutenin subunits associated to the D genome of Aegilops tauschii in a collection of synthetic hexaploid wheats. Theoretical and Applied Genetics 103: 1293–1301.CrossRefGoogle Scholar
Rasheed, A, Safdar, T, Gul-Kazi, A, Mahmood, T, Akram, Z and Mujeeb-Kazi, A (2012) Characterization of HMW-GS and evaluation of their diversity in morphologically elite synthetic hexaploid wheats. Breeding Science 62: 365–370.CrossRefGoogle ScholarPubMed
Rodríguez-Quijano, M, Nieto-Taladriz, MT and Carrillo, JM (2001) Polymorphism of high molecular weight glutenin subunits in three species of Aegilops . Genetic Resources and Crop Evolution 48: 599–607.CrossRefGoogle Scholar
Sharma, HC and Gill, BS (1983) Current status of wide hybridization in wheat. Euphytica 32: 17–31.CrossRefGoogle Scholar
Xu, SS, Khan, K, Klindworth, DL and Nygard, G (2010) Evaluation and characterization of high-molecular weight 1D glutenin subunits from Aegilops tauschii in synthetic hexaploid wheats. Journal of Cereal Science 52: 333–336.CrossRefGoogle Scholar
Yan, ZH, Wan, YF, Liu, KF, Zheng, YL and Wang, DW (2002) Identification of a novel HMW glutenin subunit and comparison of its amino acid sequence with those of homologous subunits. Chinese Science Bulletin 47: 220–225.CrossRefGoogle Scholar
Yan, Y, Hsam, SLK, Yu, JZ, Jiang, Y and Zeller, FJ (2003) Allelic variation of the HMW glutenin subunits in Aegilops tauschii accessions detected by sodium dodecyl sulphate (SDS-PAGE), acid polyacrylamide gel (A-PAGE) and capillary electrophoresis. Euphytica 130: 377–385.CrossRefGoogle Scholar
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