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Plasmodium falciparum growth inhibition by human platelets in vitro

Published online by Cambridge University Press:  06 April 2009

F. Peyron ,
B. Polack ,
D. Lamotte ,
L. Kolodie  and
P. Ambroise-Thomas
F. Peyron
Affiliation:
Laboratoire de Parasitologie (CNRS URA 1344), Faculté de Méedecine de Grenoble, 38700 La Tranche, France
B. Polack
Affiliation:
Laboratoire d'Hématologie, Centre Hospitaller Universitaire de Grenoble, BP 217X, 38043 Grenoble CEDEX, France
D. Lamotte
Affiliation:
Laboratoire d'Hématologie, Centre Hospitaller Universitaire de Grenoble, BP 217X, 38043 Grenoble CEDEX, France
L. Kolodie
Affiliation:
Laboratoire d'Hématologie, Centre Hospitaller Universitaire de Grenoble, BP 217X, 38043 Grenoble CEDEX, France
P. Ambroise-Thomas
Affiliation:
Laboratoire de Parasitologie (CNRS URA 1344), Faculté de Méedecine de Grenoble, 38700 La Tranche, France
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Summary

Platelets take an active part in immunological processes as well as in haemostasis, especially in the host-parasite relationship. Our aim is to assess the growth of Plasmodium falciparum, cultured in human erythrocytes in the presence of fresh washed human platelets, since thrombocytopaenia is frequently observed during malarial infections. Our results show that platelets induce a dose-related growth inhibition of P. falciparum. Both proliferation and maturation of intraerythrocytic stages of the parasite are inhibited. This growth inhibition is triggered by the parasite itself as neither specific antibodies nor any other components are needed to activate platelets. Activated platelets are directly toxic since complement is not involved. Furthermore, inhibition is not mediated by erythrocyte lysis or by toxic oxygen metabolites. Platelets induce an inhibition of P.falciparum growth, at least in vitro, although the importance of their role played in vivo in malarial immunity has yet to be evaluated.

Keywords

Plasmodium falciparumgrowth inhibition, platelets.platelets
Type
Research Article
Information
Parasitology , Volume 99 , Issue 3 , December 1989 , pp. 317 - 322
Copyright
Copyright © Cambridge University Press 1989

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References

Auriault, C., Pancré, V., Joseph, M., Damonneville, M., Falcoff, E. & Capron, A. (1987). The effector function of platelets is induced and regulated by T lymphocytes Annales de l'lnstitut Pasteur: Immunologie 138, 585–97.Google ScholarPubMed
Bout, D., Joseph, M., Pontet, M., Vorng, H., Deslee, D. & Capron, A. (1986). Rat resistance to schistosomiasis: platelet-mediated cytotoxicity induced by C-reactive protein. Science 231, 153–6.CrossRefGoogle ScholarPubMed
Butcher, G. A. & Clancy, R. L. (1984). Non-specific immunity to Plasmodium falciparum: in vitro studies. Transactions of the Royal Society of Tropical Medicine and Hygiene 78, 806–11.CrossRefGoogle ScholarPubMed
Cazenave, J. P., Hemmendiger, S., Beretz, A., Sutterbay, A. & Launay, J. (1983). L'agrégation plaquettaire: outil d'investigation clinique et d'étude pharmacologique: Méthodologie, Annales de Biologie Clinique 41, 167–79.Google Scholar
Celada, A., Cruchaud, A. & Perrin, L. H. (1982). Opsonic activity of human immune serum on in vitro phagocytosis of Plasmodium falciparum infected red blood cells by monocytes. Clinical and Experimental Immunology 47, 635–;44.Google ScholarPubMed
Cesbron, J. Y., Capron, A., Vargaftig, B. B., Lagarde, M., Pincemail, J., Braquet, P., Taelman, H. & Joseph, M. (1987). Platelets mediate the action of diethylcarbamazine on microfilariae. Nature, London 325, 533–6.CrossRefGoogle ScholarPubMed
Cook, G. C. (1988). Prevention and treatment of malaria. Lancet 1, 32–6.CrossRefGoogle ScholarPubMed
Crosby, W. H. & Furth, F. W. (1956). A modification of the benzidine method for measurement of haemoglobin in plasma and urine. Blood 11, 380–;3.CrossRefGoogle ScholarPubMed
Damonneville, M., Wietzerbin, J., Pancré, V., Joseph, M., Delanoye, A., Capron, A. & Auriault, C. (1988). Recombinant tumour necrosis factor mediates platelet cytotoxicity to Schistosoma mansoni larvae. Journal of Immunology 140, 3962–5.CrossRefGoogle ScholarPubMed
Essien, E. M. & Ebhota, M. I. (1981). Platelet hypersensitivity in acute malaria (Plasmodium falciparum) infection in man. Thrombosis and Haemostasis 46, 547–9.Google ScholarPubMed
Fajardo, L. F. & Tallent, C. (1973). Malarial parasites within human platelets. Journal of the American Medical Association 229, 1205–7.CrossRefGoogle Scholar
Freeman, A. L. & Holder, A. A. (1983). Surface antigens of malaria merozoites: a high molecular weight precursor is processed to 83 000 mol wt form expressed on the surface of Plasmodium falciparum merozoites. Journal of Experimental Medicine 158, 1647–53.CrossRefGoogle ScholarPubMed
Gentilini, M. & Duflo, B. (1982). Medecine Tropicale, Paludisme, (ed. Gentilini, M. & Duflo, B.), Paris: Flammarion. pp. 83–109.Google Scholar
Inyang, A. L., Sodeinde, O., Okpako, D. T. & Essien, E. M. (1987). Platelet reaction after interaction with cultured P. falciparum infected erythrocytes. British Journal of Haematology 66, 375–8.CrossRefGoogle Scholar
Jessen, T. E., Barkholt, V. & Welinder, K. G. (1983). A simple alternative pathway for haemolytic assay of human complement component C3 using methylamine-treated plasma. Journal of Immunological Methods 60, 89–100.CrossRefGoogle ScholarPubMed
Joseph, M. C., Auriault, C., Capron, A., Vorng, H. & Viens, P. (1983). A new function for platelets: IgEdependent killing of schistosomes. Nature, London 303, 810–12.CrossRefGoogle ScholarPubMed
Kharazmi, A., Jepsen, S. & Andersen, B. J. (1987). Generation of reactive oxygen radicals by human phagocytic cells activated by Plasmodium falciparum. Scandinavian Journal of Immunology 25, 335–41.CrossRefGoogle ScholarPubMed
Kueh, Y. K. & Yeo, K. L. (1982). Haematological alterations in acute malaria. Scandinavian Journal of Haematology 29, 147–52.CrossRefGoogle ScholarPubMed
Lambros, C. & Vanderberg, J. P. (1979). Synchronisation of Plasmodium falciparum erythrocytic stages in culture. Journal of Parasitology 65, 418–20.CrossRefGoogle ScholarPubMed
Mohanty, D., Marwaha, N., Ghosh, K., Sharma, S., Garewal, G., Shah, S., Devi, S. & Das, S. K. C. (1988). Functional and ultrastructural changes of platelets in malarial infection. Transactions of the Royal Society of Tropical Medicine and Hygiene 82, 369–75.CrossRefGoogle ScholarPubMed
Needleman, P., Turk, J., Jakschik, B. A., Morrison, A. R. & Lefkowith, J. B. (1986). Arachidonic acid metabolism. Annual Review of Biochemistry 55, 69–102.CrossRefGoogle ScholarPubMed
Nnalue, N. A. & Friedman, M. j. (1988) Evidence for a neutrophil-mediated protective response in malaria. Parasite Immunology 10, 47–58.CrossRefGoogle ScholarPubMed
Ockenhouse, C. F., Schulman, S. & Shear, H. L. (1984). Induction of crisis forms in the human malaria parasites Plasmodium falciparum by γ-interferonactivated, monocyte-derived macrophages. Journal of Immunology 133, 1601–8.CrossRefGoogle ScholarPubMed
Page, C. P. (1988). The involvement of platelets in nonthrombotic processes. Trends in Pharmacological Science 91, 66–71.CrossRefGoogle Scholar
Pancré, V., Joseph, M., Mazingue, C., Wietzerbin, J., Capron, A. & Auriault, C. (1987). Induction of platelet cytotoxic function by lymphokines; role of interferon-γ. Journal of Immunology 138, 4490–5.CrossRefGoogle ScholarPubMed
Slezak, s., Symer, D. E. & Shin, H. S. (1987). Plateletmediated cytotoxicity. Role of antibody and C3, and localisation of the cytotoxic system in membranes. Journal of Experimental Medicine 166, 489–505.CrossRefGoogle ScholarPubMed
Soper, W. D., Bartlett, S. P. & Winn, H. J. (1982). Lysis of antibody-coated cells by platelets. Journal of Experimental Medicine 156, 1210–21.CrossRefGoogle ScholarPubMed
Trager, W. & Jensen, J. B. (1976). Human malaria parasites in continuous culture. Science 193, 673–5.CrossRefGoogle ScholarPubMed
Viens, P., Dubois, R. & Kongshavn, P. A. L. (1983). Platelet activity in immune lysis of Trypanosoma musculi. International Journal for Parasitology 13, 527–30.CrossRefGoogle ScholarPubMed