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Initiation of chemical studies on the immunoreactive glycolipids of adult Ascaris suum

Published online by Cambridge University Press:  06 April 2009

R. D. Dennis ,
S. Baumeister ,
C. Smuda ,
C. Lochnit ,
T. Waider  and
E. Geyer
R. D. Dennis
Affiliation:
Allgemeine und Medizinische Parasitologie, FB Biologic, Philipps-Universität Marburg, Karl-von-Frisch-Straβe, D-35033 Marburg, Germany
S. Baumeister
Affiliation:
Allgemeine und Medizinische Parasitologie, FB Biologic, Philipps-Universität Marburg, Karl-von-Frisch-Straβe, D-35033 Marburg, Germany
C. Smuda
Affiliation:
Allgemeine und Medizinische Parasitologie, FB Biologic, Philipps-Universität Marburg, Karl-von-Frisch-Straβe, D-35033 Marburg, Germany
C. Lochnit
Affiliation:
Biochemisches Institut am Klinikum der Universität Gieβen, Friedrichstraβe 24, D–35385 Gieβen, Germany
T. Waider
Affiliation:
Allgemeine und Medizinische Parasitologie, FB Biologic, Philipps-Universität Marburg, Karl-von-Frisch-Straβe, D-35033 Marburg, Germany
E. Geyer
Affiliation:
Allgemeine und Medizinische Parasitologie, FB Biologic, Philipps-Universität Marburg, Karl-von-Frisch-Straβe, D-35033 Marburg, Germany
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Summary

There is a general lack of basic information concerning one class of glycoconjugate, the glycolipids, from parasitic nematodes. As the prototype, the neutral glycolipid fraction derived from adult males of Ascaris suum was investigated as to its chromatographic, differential chemical staining, antigenic and chemical properties. The thin-layer chromato-graphy-resolved neutral fraction glycolipids could be classified into components of fast and slow migrating band groups. Immunoreactivity was restricted to the latter as detected by IgG and IgM anti-neutral fraction glycolipid antibody levels in serial infection sera of mice. Similarities of chromatography, antigenicity and serological cross-reactivity have been extended to the neutral glycolipid fractions of other parasitic nematodes: Litomosoides carinii and Nippostrongylus brasiliensis. Chemical, differential chemical staining and enzymatic analyses identified the Ascaris suum antigenic, slow migrating band group of components as amphoteric glycosphingolipids, and not the originally hypothesized glyco-glycerolipids or glycosylphosphatidylinositols, that contained typical neutral monosaccharide constituents and a zwitter-ionic phosphodiester linkage, most probably phosphocholine. Glycosphingolipid-immunoreactivity is eliminated on cleavage of the zwitterionic phosphodiester linkage by hydrofluoric acid treatment.

Keywords

nematodeglycolipidsglycosphingolipidsantigenic behaviourchemical properties
Type
Research Article
Information
Parasitology , Volume 110 , Issue 5 , June 1995 , pp. 611 - 623
Copyright
Copyright © Cambridge University Press 1995

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