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Immuno-enzymatic evaluation of the recombinant TSSA-II protein of Trypanosoma cruzi in dogs and human sera: a tool for epidemiological studies

Published online by Cambridge University Press:  26 April 2011

R. O. CIMINO ,
M. MONJE RUMI ,
P. RAGONE ,
J. LAUTHIER ,
A. ALBERTI D'AMATO ,
I. R. LÓPEZ QUIROGA ,
J. F. GIL ,
S. P. CAJAL ,
N. ACOSTA  and
M. JUÁREZ
...Show all authors
R. O. CIMINO*
Affiliation:
Cátedra de Química Biológica, Facultad de Ciencias Naturales. Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina Instituto de Investigaciones de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, Alvarado 751 Orán-Salta (4530), Argentina
M. MONJE RUMI
Affiliation:
Unidad de Epidemiología Molecular-Instituto de Patología Experimental, Facultad de Ciencias de la Salud. Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
P. RAGONE
Affiliation:
Unidad de Epidemiología Molecular-Instituto de Patología Experimental, Facultad de Ciencias de la Salud. Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
J. LAUTHIER
Affiliation:
Unidad de Epidemiología Molecular-Instituto de Patología Experimental, Facultad de Ciencias de la Salud. Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
A. ALBERTI D'AMATO
Affiliation:
Unidad de Epidemiología Molecular-Instituto de Patología Experimental, Facultad de Ciencias de la Salud. Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
I. R. LÓPEZ QUIROGA
Affiliation:
Cátedra de Química Biológica, Facultad de Ciencias Naturales. Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
J. F. GIL
Affiliation:
Cátedra de Química Biológica, Facultad de Ciencias Naturales. Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina Instituto de Investigaciones de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, Alvarado 751 Orán-Salta (4530), Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina
S. P. CAJAL
Affiliation:
Instituto de Investigaciones de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, Alvarado 751 Orán-Salta (4530), Argentina
N. ACOSTA
Affiliation:
Instituto de Investigaciones de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, Alvarado 751 Orán-Salta (4530), Argentina
M. JUÁREZ
Affiliation:
Instituto de Investigaciones de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, Alvarado 751 Orán-Salta (4530), Argentina
A. KROLEWIECKI
Affiliation:
Instituto de Investigaciones de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, Alvarado 751 Orán-Salta (4530), Argentina
V. ORELLANA
Affiliation:
Cátedra de Química Biológica, Facultad de Ciencias Naturales. Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
R. ZACCA
Affiliation:
Cátedra de Microbiología, Facultad de Ciencias de la Salud de la Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina
I. MARCIPAR
Affiliation:
Laboratorio de Tecnología Inmunológica, Universidad Nacional del Litoral, Santa Fe, Ciudad Universitaria, Paraje El pozo, CC 242 (3000) Santa Fe, Argentina
P. DIOSQUE
Affiliation:
Unidad de Epidemiología Molecular-Instituto de Patología Experimental, Facultad de Ciencias de la Salud. Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina
J. R. NASSER
Affiliation:
Cátedra de Química Biológica, Facultad de Ciencias Naturales. Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina Instituto de Investigaciones de Enfermedades Tropicales, Sede Regional Orán, Universidad Nacional de Salta, Alvarado 751 Orán-Salta (4530), Argentina
*
*Corresponding author: Cátedra de Química Biológica, Facultad de Ciencias Naturales. Universidad Nacional de Salta, Av. Bolivia 5150, Salta (4400), Argentina. E-mail: [email protected]
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Summary

The rTSSA-II (recombinant Trypomastigote Small Surface II) antigen was evaluated by ELISA to detect anti-Trypanosoma cruzi antibodies in sera from naturally infected dogs and humans. For this evaluation ELISA-rTSSA-II was standardized and groups were classified according to the results obtained through xenodiagnosis, ELISA and PCR. Sensitivity (Se), Specificity (Sp), Kappa index (KI) and area under curve (AUC) were determined. The Se was determined by using 14 sera from dogs infected with T. cruzi VI (TcVI) whereas Sp was determined by using 95 non-chagasic sera by xenodiagnosis, ELISA-Homogenate and PCR. The performance of ELISA-rTSSA-II in dog sera was high (AUC=0·93 and KI=0·91). The Se was 92·85% (1 false negative) and Sp was 100%. Two sera from dogs infected with TcI and 1 with TcIII were negative. For patients infected with T. cruzi, reactivity was 87·8% (36/41), there was only 1 indeterminate, and Sp was 100%. Fifty-four sera from non-chagasic and 68 sera from patients with cutaneous leishmaniasis did not react with rTSS-II. ELISA-rTSSA-II showed a high performance when studying sera from naturally infected dogs and it also presented 100% Sp. This assay could be an important tool to carry out sero-epidemiological surveys on the prevalence of T. cruzi circulating lineages in the region.

Keywords

Trypanosoma cruziChagas' diseaselineageELISATSSA-IIdiagnosisArgentina
Type
Research Article
Information
Parasitology , Volume 138 , Issue 8 , July 2011 , pp. 995 - 1002
Copyright
Copyright © Cambridge University Press 2011

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