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Immune response to Trichinella epitopes: the antiphosphorylcholine plaque-forming cell response during the biological cycle

Published online by Cambridge University Press:  06 April 2009

F. M. Ubeira ,
J. Leiro ,
M. T. Santamarina ,
T. G. Villa  and
M. L. Sanmartín-Durán
F. M. Ubeira*
Affiliation:
Departamento de Microbiologia y Parasitología, Universidad de Santiago de Compostela, Spain
J. Leiro
Affiliation:
Departamento de Microbiologia y Parasitología, Universidad de Santiago de Compostela, Spain
M. T. Santamarina
Affiliation:
Departamento de Microbiologia y Parasitología, Universidad de Santiago de Compostela, Spain
T. G. Villa
Affiliation:
Departamento de Microbiologia y Parasitología, Universidad de Santiago de Compostela, Spain
M. L. Sanmartín-Durán
Affiliation:
Departamento de Microbiologia y Parasitología, Universidad de Santiago de Compostela, Spain
*
*Reprint requests: Dr F. M. Ubeira, Cátedra de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, Spain.
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Extract

Phosphorylcholine (PC), an immunodominant component of the cell wall of certain bacteria, fungi and nematodes, is known to induce low anti-PC antibody levels during natural infection by Trichinella spiralis. This article reports a study in which spleen cells from BCF1 mice infected with Trichinella sp. larvae were found to produce large numbers of direct haemolytic plaques in response to PC conjugated to sheep red blood cells (SRBC) after muscle-encysted larvae had been killed by treatment with mebendazole. Inhibition of the response by PC-chloride, immunodiffusion and immunoelectrophoretic studies with the anti-PC IgA (TEPC-15) and anti-idiotype T15 serum assays showed the plaque-forming cell (PFC) response to be specific for PC. The absence of haemolytic plaques when unconjugated SRBC or TNP-SRBC were used as indicator cells ruled out involvement of a polyclonal response. Greatest anti-PC PFC response was found to be associated with a microsomal fraction designated FCpl, a particulate fraction behaving as a thymus-dependent antigen. The FCpl fractions from all four strains of Trichinella employed induced anti-PC PFC responses when injected into mice. These results suggest that FCpl is a suitable antigen for use in detailed studies of immune responses to Trichinella and related parasites.

Type
Research Article
Information
Parasitology , Volume 94 , Issue 3 , June 1987 , pp. 543 - 553
Copyright
Copyright © Cambridge University Press 1987

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