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Development and survival of Ascaris suum eggs in deep litter of pigs

Published online by Cambridge University Press:  14 July 2014

KIRAN KUMAR KATAKAM ,
STIG MILAN THAMSBORG ,
NIELS CHRISTIAN KYVSGAARD ,
ANDERS DALSGAARD  and
HELENA MEJER
KIRAN KUMAR KATAKAM
Affiliation:
Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
STIG MILAN THAMSBORG
Affiliation:
Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
NIELS CHRISTIAN KYVSGAARD
Affiliation:
Section for Veterinary Medicine, Danish Health and Medicines Authority, Copenhagen, Denmark
ANDERS DALSGAARD
Affiliation:
Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
HELENA MEJER*
Affiliation:
Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
*
*Corresponding author: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlægevej 100, DK-1870 Frederiksberg C, Denmark. E-mail: [email protected]
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Summary

Indoor transmission of Ascaris suum partly depends on the physico-chemical conditions in bedding material. Temperature, pH, aqueous ammonia, moisture, occurrence and development of A. suum eggs were therefore compared in different areas (resting, intermediate and latrine) of two deep litter pens on an organic farm in four seasons. There was some variation, but mean ammonia levels were generally very low (1·0–2·6 mm) and pH levels were moderate (8·04–8·88) in all three areas. Relatively, resting areas were characterized by overall moderate moisture (36%) and moderately high temperature (35·7 °C) levels. The area contained few eggs (50 eggs g−1 DM) of which 17% were viable, and though only 4% were larvated and 0·7% appeared infective, it was more than in the other areas. Intermediate areas had moderate moisture (43%) and high temperature (43·6 °C) levels. There were many eggs (523 eggs g−1 DM), but overall viability was very low (5%) and few eggs were larvated (0·004%) or even infective (0·002%). Latrines typically had high moisture (79%) and moderate temperature (30 °C) levels. The concentration of eggs was very high (1444 egg g−1 DM) and though 32% were viable, none had developed larval stages. The large majority of A. suum eggs appear to die and only few become infective while in the deep litter. However, a large fraction of eggs may remain viable for some time and could thus contaminate agricultural land and develop to infectivity, if the manure is not composted appropriately.

Keywords

deep litterbedding materialAscaris suum eggsdevelopmentviabilitytemperatureammoniapHmoisture
Type
Research Article
Information
Parasitology , Volume 141 , Issue 12 , October 2014 , pp. 1646 - 1656
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Copyright
Copyright © Cambridge University Press 2014 

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References

REFERENCES

Arene, F. O. I. (1986). Ascaris suum: influence of embryonation temperature on the viability of the infective larva. Journal of Thermal Biology 11, 9–15.Google Scholar
Armstrong, D. A., Chippendale, D., Knight, A. W. and Colt, J. E. (1978). Interaction of ionized and un-ionized ammonia on short-term survival and growth of prawn larvae, Macrobrachium rosenbergh. Biological Bulletin 154, 15–31.Google Scholar
Barnard, R. J., Bier, J. W., Jackson, G. J. and McClure, F. D. (1987). Ascaris lumbricoides suum: thermal death time of unembryonated eggs. Experimental Parasitology 64, 120–122.CrossRefGoogle ScholarPubMed
Barrett, J. (1976). Studies on the induction of permeability in Ascaris lumbricoides eggs. Parasitology 73, 109–121.CrossRefGoogle ScholarPubMed
Bernal, M., Alburquerque, J. and Moral, R. (2009). Composting of animal manures and chemical criteria for compost maturity assessment. A review. Bioresource Technology 100, 5444–5453.Google Scholar
Connan, R. (1977). Ascariasis: the development of eggs of Ascaris suum under the conditions prevailing in a pig house. Veterinary Record 100, 421–422.Google Scholar
de Bertoldi, M., Vallini, G. and Pera, A. (1983). The biology of composting: a review. Waste Management and Research 1, 157–176.CrossRefGoogle Scholar
EPA. (1994). Composting yard trimmings and municipal solid waste. Office of Solid Waste and Emergency Response (EPA 530-R-94-003), Washington, DC, USA.Google Scholar
Eriksen, L., Nansen, P., Roepstorff, A., Lind, P. and Nilsson, O. (1992). Response to repeated inoculations with Ascaris suum eggs in pigs during the fattening period. Parasitology Research 78, 241–246.Google Scholar
Eriksen, L., Andreasen, P. and Ilsøe, B. (1996). Inactivation of Ascaris suum eggs during storage in lime treated sewage sludge. Water Research 30, 1026–1029.Google Scholar
Gantzer, C., Gaspard, P., Galvez, L., Huyard, A., Dumouthier, N. and Schwartzbrod, J. (2001). Monitoring of bacterial and parasitological contamination during various treatment of sludge. Water Research 35, 3763–3770.Google Scholar
Gentry, J., McGlone, J., Miller, M. and Blanton, J. (2004). Environmental effects on pig performance, meat quality, and muscle characteristics. Journal of Animal Science 82, 209–217.Google Scholar
GraphPad Prism version 6.02 for Windows. (2013). GraphPad Software, San Diego, CA, USA, www.graphpad.com.Google Scholar
Groenestein, C. and Van Faassen, H. (1996). Volatilization of ammonia, nitrous oxide and nitric oxide in deep-litter systems for fattening pigs. Journal of Agricultural Engineering Research 65, 269–274.Google Scholar
Guy, J. H. and Edwards, S. (2006). Alternative production systems. In Livestock Production and Society (eds. Geersand, R. and Madec, F.), pp. 273–286. Wageningen Academic Publishers, Wageningen, the Netherlands.Google Scholar
Hill, J. D., McGlone, J. J., Fullwood, S. D. and Miller, M. F. (1998). Environmental enrichment influences on pig behavior, performance and meat quality. Applied Animal Behaviour Science 57, 51–68.Google Scholar
Holmgren, N. and Nilsson, O. (1998). Inverkan av produktionsplanering och skabbsanering på tarmnematoder hos bis-grisar. Svenska Djurhälsovården. Slutrapport till Köttböndernas Forskningsprogram projektnr G37–G97.Google Scholar
Honeyman, M. (2005). Extensive bedded indoor and outdoor pig production systems in USA: current trends and effects on animal care and product quality. Livestock Production Science 94, 15–24.Google Scholar
Jorgensen, K. and Jensen, L. S. (2009). Chemical and biochemical variation in animal manure solids separated using different commercial separation technologies. Bioresource Technology 100, 3088–3096.CrossRefGoogle ScholarPubMed
Jungersen, G., Eriksen, L., Roepstorff, A., Lind, P., Meeusen, E. N., Rasmussen, T. and Nansen, P. (1999). Experimental Ascaris suum infection in the pig: protective memory response after three immunizations and effect of intestinal adult worm population. Parasite Immunology 21, 619–630.CrossRefGoogle ScholarPubMed
Kalyuzhnyi, S., Sklyar, V., Fedorovich, V., Kovalev, A., Nozhevnikova, A. and Klapwijk, A. (1999). The development of biological methods for utilisation and treatment of diluted manure streams. Water Science and Technology 40, 223–229.Google Scholar
Katakam, K. K., Roepstorff, A., Popovic, O., Kyvsgaard, N. C., Thamsborg, S. M. and Dalsgaard, A. (2013). Viability of Ascaris suum eggs in stored raw and separated liquid slurry. Parasitology 140, 378–384.Google Scholar
Katakam, K. K., Mejer, H., Dalsgaard, A., Kyvsgaard, N. C. and Thamsborg, S. M. (2014). Survival of Ascaris suum and Ascaridia galli eggs in liquid manure at different ammonia concentrations and temperatures. Veterinary Parasitology. doi: 10.1016/j.vetpar.2014.05.017.Google Scholar
Kirchmann, H. and Witter, E. (1989). Ammonia volatilization during aerobic and anaerobic manure decomposition. Plant and Soil 115, 35–41.Google Scholar
Krishnaswami, S. K. and Post, F. J. (1968). Effect of chlorine on Ascaris (Nematoda) eggs. Health Laboratory Science 5, 225–232.Google Scholar
Kunte, D., Yeole, T. and Ranade, D. (2004). Two-stage anaerobic digestion process for complete inactivation of enteric bacterial pathogens in human night soil. Water Science and Technology 50, 103–108.Google Scholar
Larsen, M. N. and Roepstorff, A. (1999). Seasonal variation in development and survival of Ascaris suum and Trichuris suis eggs on pastures. Parasitology 119, 209–220.Google Scholar
Martini, M., Poglayen, G. and Mancini, B. (1988). Epidemiological investigations of Ascariasis in pigs. Estratto de Selezione Veterinaria 29, 981–986.Google Scholar
Mejer, H. and Roepstorff, A. (2006). Ascaris suum infections in pigs born and raised on contaminated paddocks. Parasitology 133, 305–312.Google Scholar
Menzies, F., Goodall, E. and Taylor, S. (1994). The epidemiology of Ascaris sum infections in pigs in Northern Ireland, 1969–1991. British Veterinary Journal 150, 165–172.Google Scholar
Morrison, R. S., Johnston, L. J. and Hilbrands, A. M. (2007). The behaviour, welfare, growth performance and meat quality of pigs housed in a deep-litter, large group housing system compared to a conventional confinement system. Applied Animal Behaviour Science 103, 12–24.Google Scholar
Mosher, D. and Anderson, R. (1977). Composting sewage sludge by high-rate suction aeration techniques. US Environmental Protection Agency, Interim Report No. SW-614d.Google Scholar
Nilsson, O. (1982). Ascariasis in the pig. An epizootiological and clinical study. Acta Veterinaria Scandinavica. Supplementum 79, 1–108.Google Scholar
Nordin, A., Nyberg, K. and Vinnerås, B. (2009). Inactivation of Ascaris eggs in source-separated urine and feces by ammonia at ambient temperatures. Applied and Environmental Microbiology 75, 662–667.Google Scholar
O'Donnell, C. J., Meyer, K. B., Jones, J. V., Benton, T., Kaneshiro, E. S., Nichols, J. S. and Schaefer, F. W. 3rd, (1984). Survival of parasite eggs upon storage in sludge. Applied and Environmental Microbiology 48, 618–625.Google Scholar
Oksanen, A., Eriksen, L., Roepstorff, A., Ilsoe, B., Nansen, P. and Lind, P. (1990). Embryonation and infectivity of Ascaris suum eggs. A comparison of eggs collected from worm uteri with eggs isolated from pig faeces. Acta Veterinaria Scandinavica 31, 393–398.Google Scholar
Pecson, B. M., Barrios, J. A., Jiménez, B. E. and Nelson, K. L. (2007). The effects of temperature, pH, and ammonia concentration on the inactivation of Ascaris eggs in sewage sludge. Water Research 41, 2893–2902.Google Scholar
Petit, J. and van der Werf, H. M. G. (2003). Perception of the environmental impacts of current and alternative modes of pig production by stakeholder groups. Journal of Environmental Management 68, 377–386.Google Scholar
Petric, I., Šestan, A. and Šestan, I. (2009). Influence of initial moisture content on the composting of poultry manure with wheat straw. Biosystems Engineering 104, 125–134.Google Scholar
Philippe, F., Cabaraux, J. and Nicks, B. (2011). Ammonia emissions from pig houses: influencing factors and mitigation techniques. Agriculture, Ecosystems and Environment 141, 245–260.Google Scholar
Regan, R., Jeris, J. S., Basser, R., McCann, K. and Hudek, J. (1973). Cellulose Degradation in Composting. EPA-R3-73-029, PB 215 722, p. 142. US EPA, Washington, DC, USA.Google Scholar
Roepstorff, A. (1997). Helminth surveillance as a prerequisite for anthelmintic treatment in intensive sow herds. Veterinary Parasitology 73, 139–151.CrossRefGoogle ScholarPubMed
Roepstorff, A. and Murrell, K. (1997). Transmission dynamics of helminth parasites of pigs on continuous pasture: Ascaris suum and Trichuris suis. International Journal for Parasitology 27, 563–572.Google Scholar
Roepstorff, A. and Nansen, P. (1998). Epidemiology, Diagnosis and Control of Helminth Parasites of Swine. FAO Animal Health Manual No 3. FAO, Rome, Italy.Google Scholar
Roepstorff, A., Jørgensen, R., Nansen, P., Henriksen, S., Skovgaard, J., Pedersen, J. and Andreasen, M. (1992). Parasites in organic pigs. Rapport over Projekt finansieret af Jordbrugsdirektoratet under Landbrugsministeriet. National Committee for Pig Production, Danish Bacon and Meat Council, Copenhagen, 36.Google Scholar
Roepstorff, A., Eriksen, L., Slotved, H. and Nansen, P. (1997). Experimental Ascaris suum infection in the pig: worm population kinetics following single inoculations with three doses of infective eggs. Parasitology 115, 443–452.Google Scholar
Roepstorff, A., Nilsson, O., Oksanen, A., Gjerde, B., Richter, S., Örtenberg, E., Christensson, D., Martinsson, K., Bartlett, P. and Nansen, P. (1998). Intestinal parasites in swine in the Nordic countries: prevalence and geographical distribution. Veterinary Parasitology 76, 305–319.Google Scholar
R Development Core Team. (2010). R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria. Retrieved from http://R-project.org.Google Scholar
Sanguinetti, G., Tortul, C., Garcia, M., Ferrer, V., Montangero, A. and Strauss, M. (2005). Investigating helminth eggs and Salmonella sp. in stabilization ponds treating septage. Water Science and Technology 51, 239–247.Google Scholar
SAS Institute Inc. (2008). SAS Version 9.2 for Windows. SAS Institute Inc., Cary, NC, USA.Google Scholar
Seamster, A. P. (1950). Developmental studies concerning the eggs of Ascaris lumbricoides var. suum. American Midland Naturalist 43, 450–470.Google Scholar
Sommer, S. G. and Moller, H. (2000). Emission of greenhouse gases during composting of deep litter from pig production-effect of straw content. Journal of Agricultural Science 134, 327–335.Google Scholar
Stevenson, P. (1979). The influence of environmental temperature on the rate of development of Ascaris suum eggs in Great Britain. Research in Veterinary Science 27, 193–196.Google Scholar