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Usefulness of gel filtration fraction as potential biomarker for neurocysticercosis in serum: towards a new diagnostic tool

Published online by Cambridge University Press:  17 October 2016

D. S. NUNES ,
H. T. GONZAGA ,
V. S. RIBEIRO ,
J. P. CUNHA-JÚNIOR  and
J. M. COSTA-CRUZ
D. S. NUNES
Affiliation:
Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil
H. T. GONZAGA
Affiliation:
Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil
V. S. RIBEIRO
Affiliation:
Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil Unidade Acadêmica Especial de Biotecnologia, Área de Ciências Biológicas, Universidade Federal de Goiás, Regional Catalão, Av. Dr. Lamartine Pinto Avelar 1120, Bloco M, 75704-020, Catalão, GO, Brazil
J. P. CUNHA-JÚNIOR
Affiliation:
Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil
J. M. COSTA-CRUZ*
Affiliation:
Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, 38400-902, Uberlândia, MG, Brazil
*
*Corresponding author: Laboratório de Diagnóstico de Parasitoses, Departamento de Imunologia, Microbiologia e Parasitologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG, Brasil. Avenida Pará 1720, 38400-902, Uberlândia, MG, Brazil. E-mail: [email protected]
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Summary

There is an increasing interest in improving neurocysticercosis (NCC) diagnosis through the search of new and alternative antigenic sources, as those obtained from heterologous antigens. The aim of this study was to obtain potential biomarkers for NCC diagnosis after gel filtration chromatography [gel filtration fraction (GFF)] from the total saline extract (SE) from Taenia saginata metacestodes, followed by protein identification and application in immunodiagnostic. SE and GFF proteic profiles were characterized in gel electrophoresis, and diagnostic performance was verified by testing 160 serum samples through enzyme-linked immunosorbent assay and immunoblotting. Sensitivity (Se), specificity (Sp) and other diagnostic parameters were calculated. Polypeptides of interest in the diagnosis of human NCC present at GFF were analysed by mass spectrometry (MS) and B-cell epitopes were predicted. GFF had the best diagnostic parameters: Se 93·3%; Sp 93%; AUC 0·990; LR+ = 13·42 and LR− = 0·07, and proved to be useful reacting with serum samples in immunoblotting. Proteic profile ranged from 64 to 68 kDa and enolase and calcium binding protein calreticulin precursor were identified after MS. The enolase and calcium-binding protein calreticulin precursor showed 18 and 10 predicted B-cell epitopes, respectively. In conclusion we identified important markers in the GFF with high efficiency to diagnose NCC.

Keywords

gel filtration fractionimmunodiagnosisneurocysticercosisproteomicTaenia saginata
Type
Research Article
Information
Parasitology , Volume 144 , Issue 4 , April 2017 , pp. 426 - 435
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Copyright
Copyright © Cambridge University Press 2016 

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