Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-12-01T00:51:21.871Z Has data issue: false hasContentIssue false
  • English
  • Français

The tegument of Schistosoma mansoni: a histochemical investigation

Published online by Cambridge University Press:  06 April 2009

P. R. Wheater  and
R. A. Wilson
P. R. Wheater
Affiliation:
Department of Biology, University of York, Heslington, York
R. A. Wilson
Affiliation:
Department of Biology, University of York, Heslington, York
Get access Rights & Permissions [Opens in a new window]

Summary

The main components of the schistosome tegument were found to be neutral glycoprotein and phospholipid; a small quantity of glycolipid was observed in the male dorsal tegument. The tegument can be differentiated from other schistosome tissues on the basis of enzyme content; three hydrolytic enzymes were shown to be specifically localized in the tegument: alkaline phosphatase, adenosine triphosphatase and indoxyl esterase. It is suggested that these enzymes could be used as intrinsic markers for tegument structures. The subtegumental cells appear to be the major sites of biosynthetic activity since they contain large amounts of RNA and mitochondrial enzymes.

Type
Research Article
Information
Parasitology , Volume 72 , Issue 1 , February 1976 , pp. 99 - 109
Copyright
Copyright © Cambridge University Press 1976

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bancroft, J. D. (1967). An Introduction to Histochemical Technique. London: Butterworth.Google Scholar
Bogitsh, B. J. & Krupa, P. (1971). Schistosoma mansoni and Haematoloechus medioplexus: nucleoside-diphosphatase localisation in tegument. Experimental Parasitology 30, 418–25.Google Scholar
Clegg, J. (1972). Functional aspects of parasite surfaces, British Society for Parasitology Symposium, no. 10 (ed. Taylor, A. E. R. and Muller, B.), pp. 23–40. London: Blackwell.Google Scholar
Dusanic, D. G. (1959). Histochemical observations of alkaline phosphatase in Schistosoma mansoni. Journal of Infectious Diseases 105, 1–8.CrossRefGoogle ScholarPubMed
Fripp, P. J. (1966). The histochemical localisation of β-glucuronidase in schistosomes. Experimental Parasitology 19, 254–63.CrossRefGoogle ScholarPubMed
Fripp, P. J. (1967 a). The histochemical localisation of esterase activity in schistosomes. Experimental Parasitology 21, 380–90.CrossRefGoogle ScholarPubMed
Fripp, P. J. (1967 b). The histochemical localisation of leucine aminopeptidase in Schistosoma rodhaini. Comparative Biochemistry and Physiology 20, 307–9.CrossRefGoogle Scholar
Halton, D. W. (1967). Studies on phosphatase activity in Trematoda. Journal of Parasitology 53, 46–54.CrossRefGoogle ScholarPubMed
Hockley, D. J. & McLaren, D. J. (1973). Schistosoma mansoni: changes in the outer membrane of the tegument during development from cercaria to adult worm. International Journal for Parasitology 3, 13–25.CrossRefGoogle ScholarPubMed
Kusel, J. (1972). Protein composition and protein synthesis in surface membranes of Schistosoma mansoni. Parasitology 65, 55–9.CrossRefGoogle ScholarPubMed
Lee, D. L. (1966). The structure and composition of the helminth cuticle. Advances in Parasitology 4, 187–254.CrossRefGoogle ScholarPubMed
Lewert, R. M. & Dusanic, D. G. (1961). Effects of a symmetrical diamino-dibenzyl-alkene on alkaline phosphatase of Schistosoma mansoni. Journal of Infectious Diseases 109, 85–9.CrossRefGoogle ScholarPubMed
Lodja, Z. (1974). Cytochemistry of enterocytes and of other cells in the mucous membrane of the small intestine. In Biomembranes, Vol. 4A. Intestinal Absorption (ed. Smyth, D. H.), pp. 43–122. London: Plenum Press.Google Scholar
McManus, J. F. A. & Mowry, R. W. (1960). Staining Methods. London: Harper and Row.Google Scholar
Meyer, F., Meyer, H. & Beuding, E. (1970). Lipid metabolism in the parasitic and free-living flatworms, Schistosoma mansoni and Dugesia dorotocephala. Biochimica et Biophysica Acta-210, 257–66.CrossRefGoogle ScholarPubMed
Morris, G. P. & Threadgold, L. T. (1968). Ultrastructure of the tegument of Schistosoma mansoni. Journal of Parasitology 54, 15–27.Google ScholarPubMed
Nimmo-Smith, R. H. & Standen, O. D. (1963). Phosphomonoesterases of Schistosoma mansoni. Experimental Parasitology 13, 305–22.CrossRefGoogle ScholarPubMed
Padykula, H. A. & Herman, E. (1955). Specificity of histochemical method for adenosine triphosphatase. Journal of Histochemistry and Cytochemistry 3, 170–95.CrossRefGoogle ScholarPubMed
Pearse, A. G. E. (1968). Histochemistry, Theoretical and Applied, 2nd ed. London: Churchill.Google Scholar
Robinson, D. L. H. (1961). Phosphatase in Schistosoma mansoni. Nature, London 191, 473–4.CrossRefGoogle ScholarPubMed
Smith, J. H., Reynolds, E. S. & von Lichtenberg, F. (1969). The integument of Schistosoma mansoni. American Journal of Tropical Medicine and Hygine 18, 28–49.CrossRefGoogle ScholarPubMed
Wilson, R. A. & Barnes, P. E. (1974 a). The tegument of Schistosoma mansoni: observations on the formation, structure and composition of cytoplasmic inclusions in relation to tegument function. Parasitology 68, 239–58.CrossRefGoogle ScholarPubMed
Wilson, R. A. & Barnes, P. E. (1974 b). An in vitro investigation of dynamic processes occurring in the schistosome tegument using compounds known to disrupt secretory processes. Parasitology 68, 259–70.CrossRefGoogle Scholar