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Repetitive DNA and hybridization patterns demonstrate extensive variability between the sibling species Globodera rostochiensis and G. pallida

Published online by Cambridge University Press:  06 April 2009

A. J. De Jong ,
J. Bakker ,
M. Roos  and
F. J. Gommers
A. J. De Jong
Affiliation:
Department of Nematology, Agricultural University, Binnenhaven 10, 6709 PD Wageningen, The Netherlands
J. Bakker
Affiliation:
Department of Nematology, Agricultural University, Binnenhaven 10, 6709 PD Wageningen, The Netherlands
M. Roos
Affiliation:
Division of Parasitology, University of Utrecht, Yalelaan 1, P.O. Box 80.171, 3508 TD Utrecht, The Netherlands
F. J. Gommers
Affiliation:
Department of Nematology, Agricultural University, Binnenhaven 10, 6709 PD Wageningen, The Netherlands
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Summary

A method is described for the isolation of high molecular weight DNA from females of potato cyst nematodes. Restriction enzyme analyses of repetitive DNA revealed 30 bands specific for Globodera rostochiensis and 8 bands specific for G. pallida. None of the repetitive DNA bands resolved was common to both species. Hybridization patterns, obtained with a DrosophilaβJ-tubulin probe, revealed 4 common bands, 3 bands specific for G. rostochiensis and 12 bands specific for G. pallida.

Keywords

Globodera roslochiensisGlobodera pallidaDNAhybridizationpatterns.
Type
Research Article
Information
Parasitology , Volume 99 , Issue 1 , August 1989 , pp. 133 - 138
Copyright
Copyright © Cambridge University Press 1989

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