Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-29T00:45:10.643Z Has data issue: false hasContentIssue false
  • English
  • Français

Preliminary biochemical characterization of ‘veil’ structure purified from Theileria sergenti-, T. buffeli- and T. orientalis-infected bovine erythrocytes

Published online by Cambridge University Press:  06 April 2009

C. Sugimoto ,
S. Kawazu ,
M. Sato ,
T. Kamio  and
K. Fujisaki
C. Sugimoto
Affiliation:
National Institute of Animal Health, Tsukuba, Ibaraki 305, Japan
S. Kawazu
Affiliation:
National Institute of Animal Health, Tsukuba, Ibaraki 305, Japan
M. Sato
Affiliation:
National Institute of Animal Health, Tsukuba, Ibaraki 305, Japan
T. Kamio
Affiliation:
National Institute of Animal Health, Tsukuba, Ibaraki 305, Japan
K. Fujisaki
Affiliation:
National Institute of Animal Health, Tsukuba, Ibaraki 305, Japan
Get access Rights & Permissions [Opens in a new window]

Summary

A structure called ‘veil’ in Theileria sergenti-, T. buffeli- or T. orientalis-infected bovine erythrocytes was purified from erythrocyte lysates by Percoll density-gradient centrifugation. On electron microscopical examination, the veils consisted of electron-dense material showing a periodicity of striations and were not surrounded by membranes. Analyses of veil proteins by one- and two-dimensional polyacrylamide gel electrophoresis revealed that the veils contain haemoglobin and several other basic proteins of molecular weight ranging from 15·5 to 46 kDa. By comparing the protein patterns of the veil with those of purified piroplasms and uninfected bovine erythrocytes, these basic proteins appeared to be of parasite origin. It would appear likely that the veils formed by intra-erythrocytic precipitation of haemoglobin and proteins excreted by the parasites. Differences in veil constituents were found between T. sergenti, T. buffeli and T. orientalis.

Keywords

Theileriapiroplasmveilerythrocyte
Type
Research Article
Information
Parasitology , Volume 104 , Issue 2 , April 1992 , pp. 207 - 213
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Anderson, N. G. & Anderson, N. L. (1978 a). Analytical techniques for cell fractions. XXI. Two-dimensional analysis of serum and tissue proteins: multiple isoelectric focusing. Analytical Biochemistry 85, 331–40.CrossRefGoogle ScholarPubMed
Anderson, N. L. & Anderson, N. G. (1978 b). Analytical techniques for cell fractions. XXI. Two-dimensional analysis of serum and tissue proteins: multiple gradient slab gel electrophoresis. Analytical Biochemistry 85, 341–54.CrossRefGoogle Scholar
Asao, T., Kinoshita, Y., Kozaki, S., Uemura, T. & Sakaguchi, G. (1984). Purification and some properties of Aeromonas hydrophila hemolysin. Infection and Immunity 46, 122–7.CrossRefGoogle ScholarPubMed
Edwards, J. J., Anderson, N. G., Nance, S. L. & Anderson, N. L. (1979). Red cell proteins. I. Two-dimensional mapping of human erythrocyte lysate proteins. Blood 53, 1121–32.CrossRefGoogle ScholarPubMed
Fawcett, D. W., Conrad, P. A., Grootenhuis, J. G. & Morzaria, S. P. (1987). Ultrastructure of the intraerythrocytic stage of Theileria species from cattle and waterbuck. Tissue and Cell 19, 643–55.CrossRefGoogle ScholarPubMed
Fujisaki, K., Ito, Y., Kamio, T. & Kitaoka, S. (1985). The presence of Theileria sergenti in Haemaphysalis longicornis overwintering in pasture in Japan. Annals of Tropical Medicine and Parasitology 79, 519–34.CrossRefGoogle ScholarPubMed
Fujisaki, K., Kamio, T. & Kawazu, S. (1991). Theileria sergenti cannot be regarded as the same species as T. buffeli and T. orientalis because of its transmissibility only by Kaiseriana ticks. In Modern Acarology (ed. Dusbabek, F.). Prague: Publishing House Academia. (In the Press.)Google Scholar
Kawai, S., Takahashi, K., Kawamoto, S., Nagahara, A., Sonoda, M., Kurosawa, T. & Onuma, M. (1989). Bar-structure in bovine erythrocytes infected with Theileria sergenti. Japanese Journal of Veterinary Science 51, 1219–25.Google ScholarPubMed
Kawazu, S., Sugimoto, C., Kamio, T. & Fujisaki, K. (1991). Antigenic differences between Japanese Theileria sergenti and other Theileria species of cattle from Australia (T. buffeli) and Britain (T. orientalis). Parasitology Research (in the Press).Google Scholar
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–5.CrossRefGoogle ScholarPubMed
Low, P. S., Zinke, K. & Drenckhahn, D. (1985). The role of hemoglobin denaturation and band 3 clustering in red cell aging. Science 227, 531–3.CrossRefGoogle Scholar
Morzaria, S. P., Barnett, S. F. & Brocklesby, D. W. (1974). Isolation of Theileria mutans from cattle in Essex. Veterinary Record 94, 256.CrossRefGoogle ScholarPubMed
Schlüter, K. & Drenckhahn, D. (1986). Co-clustering of denatured hemoglobin with band 3: its role in binding of autoantibodies against band 3 to abnormal and aged erythrocytes. Proceedings of the National Academy of Sciences, USA 83, 6137–41.CrossRefGoogle ScholarPubMed
Schofield, L. (1991). On the function of repetitive domains in the protein antigens of Plasmodium and other eukaryotic parasites. Parasitology Today 7, 99–105.CrossRefGoogle ScholarPubMed
Slater, F. G., Swiggard, W. J., Orton, B. R., Flitter, W. D., Goldberg, D. E., Cerami, A. & Henderson, G. B. (1991). An iron-carboxylate bond links the heme units of malaria pigment. Proceedings of National Academy of Sciences, USA 88, 325–9.CrossRefGoogle ScholarPubMed
Stewart, N. P., De Vos, A. J., McGregor, W. & Shiels, I. (1988). Observations on the development of tick-transmitted Theileria buffeli (syn T. orientalis?) in cattle. Research in Veterinary Science 44, 338–42.CrossRefGoogle Scholar
Stewart, N. P., De Vos, A. J., Shields, I. & McGregor, W. (1987). The experimental transmission of Theileria buffeli of cattle in Australia by Haemaphysalis humerosa. Australian Veterinary Journal 64, 81–3.CrossRefGoogle ScholarPubMed
Sugimoto, C., Kawazu, S., Kamio, T. & Fujisaki, K. (1991 a). Protein analysis of Theileria sergenti/buffeli/orientalis piroplasms by two-dimensional polyacrylamide gel electrophoresis. Parasitology 102, 341–6.CrossRefGoogle ScholarPubMed
Sugimoto, C., Sato, M., Kawazu, S., Kamio, T. & Fujisaki, K. (1991 b). Purification of merozoites of Theileria sergenti from infected bovine erythrocytes. Parasitology Research 77, 129–31.CrossRefGoogle ScholarPubMed
Takao, T., Sonoda, M., Takahashi, K. & Kurosawa, T. (1983). Studies of the rod-bodies in the erythrocytes of cows affected with Theileria sergenti. Journal of the College of Dairying 10, 183–96. (In Japanese with English summary.)Google Scholar
Uilenberg, G. (1981). Theilerial species of domestic livestock. In Advances in the Control of Theileriosis (ed. Irvin, A. D., Cunningham, M. P. & Young, A. S.), pp. 4–37. The Hague: Martinus Nijhoff.CrossRefGoogle Scholar
Uilenberg, G. (1964). Haemotoxenus veliferus n.g., n.sp., parasite incertae sedis du sang de bovins à Madagascar. Revue d'elevage et de Médicine Vétérinaire des pays Tropicaux 17, 655–62.Google Scholar
Uilenberg, G. & Andreasen, M. P. (1974). Haematoxenus separatus sp.n. (Sporozoa, Theileriidae), a new blood parasite of domestic sheep in Tanzania. Revue d'elevage et de Médicine Vétérinaire des pays Tropicaux 27, 459–65.Google Scholar
Uilenberg, G., Períe, N. M., Spanjer, A. A. M. & Franssen, F. F. J. (1985). Theileria orientalis, a cosmopolitan blood parasite of cattle: demonstration of the schizont stage. Research in Veterinary Science 38, 352–7.CrossRefGoogle ScholarPubMed
Van Vorstenbosch, C. A. A. H., Uilenberg, G. & Van Dijik, J. E. (1978). Erythrocytic forms of Theileria velifera. Research in Veterinary Science 24, 214–21.CrossRefGoogle ScholarPubMed
Winterbourn, C. C. & Carrell, R. W. (1974). Studies of hemoglobin denaturation and Heinz body formation in the unstable hemoglobins. Journal of Clinical Investigations 54, 678–89.CrossRefGoogle ScholarPubMed
Yamada, K. A. & Sherman, I. W. (1979). Plasmodium iophurae: Composition and properties of hemozoin, the malarial pigment. Experimental Parasitology 48, 61–74.CrossRefGoogle Scholar
Young, A. S., Grootenhuis, J. G., Smith, K., Flowers, M. J., Dolan, T. T. & Brocklesby, D. W. (1978). Structure associated with Theileria parasites in eland erythrocytes. Annals of Tropical Medicine and Parasitology 72, 443–54.CrossRefGoogle ScholarPubMed