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Plasticity of gp63 gene organization in Leishmania (Viannia) braziliensis and Leishmania (Viannia) peruviana

Published online by Cambridge University Press:  06 April 2009

K. Victoir ,
J. C. Dujardin ,
S. De Doncker ,
D. C. Barker ,
J. Arevalo ,
R. Hamers  and
D. Le Ray
K. Victoir
Affiliation:
Instituut voor Tropische Geneeskunde‘Prince Leopold’, 155 Nationalestraat, B-2000 Antwerpen, Belgium
J. C. Dujardin
Affiliation:
Instituut voor Tropische Geneeskunde‘Prince Leopold’, 155 Nationalestraat, B-2000 Antwerpen, Belgium
S. De Doncker
Affiliation:
Instituut voor Tropische Geneeskunde‘Prince Leopold’, 155 Nationalestraat, B-2000 Antwerpen, Belgium
D. C. Barker
Affiliation:
Cambridge University, Department of Pathology, Tennis Court Road, Cambridge CB2 1QP, UK
J. Arevalo
Affiliation:
Universidad Peruana Cayetano Heredia, AP 4314, Lima 100, Peru
R. Hamers
Affiliation:
Vrije Universiteit Brussel, Paardenstraat 65, B-1640 Sint Genesius Rode, Belgium
D. Le Ray
Affiliation:
Instituut voor Tropische Geneeskunde‘Prince Leopold’, 155 Nationalestraat, B-2000 Antwerpen, Belgium
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Summary

The genomic organization of gp63 genes in 4 and 7 isolates of Leishmania braziliensis and L. peruviana, respectively was studied by RFLP analysis with 3 restriction enzymes (Bgl I, Sal I and Apa I). Our results showed a marked polymorphism among isolates. Some characters were specific to L. braziliensis or to L. peruviana, and others specific to the respective biogeographical populations of L. peruviana. The average minimum copy number of gp63 genes was found to be higher in L. braziliensis (71) than in L. peruviana (46), suggesting that deletion of gp63 genes might be partially involved in the size decrease of the chromosome bearing gp63 genes, observed between those 2 species (from 700 to 610 kb). Our results may suggest the existence of at least 2 arrays of heterologous gp63 repeats, varying in relative copy number between L. braziliensis and L. peruviana, and among isolates of the latter species. Rearrangement of the gp63 genes was observed during long-term in vitro maintenance of a reference strain of L. braziliensis. These observations document the existence of a dynamic gp63 gene organization in Leishmania of the braziliensis complex.

Keywords

genomic organizationLeishmania braziliensisLeishmania peruvianagp63 gene
Type
Research Article
Information
Parasitology , Volume 111 , Issue 3 , September 1995 , pp. 265 - 273
Copyright
Copyright © Cambridge University Press 1995

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