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A new microsporidium Percutemincola moriokae gen. nov., sp. nov. from Oscheius tipulae: A novel model of microsporidia–nematode associations

Published online by Cambridge University Press:  17 April 2018

Kenji Nishikori Open the ORCID record for Kenji Nishikori [Opens in a new window] ,
Davin H. E. Setiamarga ,
Takahiro Tanji ,
Eisuke Kuroda ,
Hirohisa Shiraishi  and
Ayako Ohashi-Kobayashi
Kenji Nishikori*
Affiliation:
Department of Immunobiology, School of Pharmacy, Iwate Medical University, Nishitokuta 2-1-1, Yahaba, Shiwa, Iwate, Japan
Davin H. E. Setiamarga
Affiliation:
Department of Applied Chemistry and Biochemistry, National Institute of Technology, Wakayama College, Noda, Noshima 77, Gobo City, Wakayama, Japan The University Museum, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, Japan
Takahiro Tanji
Affiliation:
Department of Immunobiology, School of Pharmacy, Iwate Medical University, Nishitokuta 2-1-1, Yahaba, Shiwa, Iwate, Japan
Eisuke Kuroda
Affiliation:
Department of Immunobiology, School of Pharmacy, Iwate Medical University, Nishitokuta 2-1-1, Yahaba, Shiwa, Iwate, Japan
Hirohisa Shiraishi
Affiliation:
Department of Immunobiology, School of Pharmacy, Iwate Medical University, Nishitokuta 2-1-1, Yahaba, Shiwa, Iwate, Japan
Ayako Ohashi-Kobayashi
Affiliation:
Department of Immunobiology, School of Pharmacy, Iwate Medical University, Nishitokuta 2-1-1, Yahaba, Shiwa, Iwate, Japan
*
Author for correspondence: Kenji Nishikori, E-mail: [email protected]
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Abstract

Here, we describe a new microsporidium Percutemincola moriokae gen. nov., sp. nov., which was discovered in the intestinal and hypodermal cells of a wild strain of the nematode Oscheius tipulae that inhabits in the soil of Morioka, Iwate Prefecture, Japan. The spores of Pe. moriokae had an average size of 1.0 × 3.8 µm and 1.3 × 3.2 µm in the intestine and hypodermis, respectively, and electron microscopy revealed that they exhibited distinguishing features with morphological diversity in the hypodermis. Isolated spores were able to infect a reference strain of O. tipulae (CEW1) through horizontal transmission but not the nematode Caenorhabditis elegans. Upon infection, the spores were first observed in the hypodermis and then in the intestine the following day, suggesting a unique infectious route among nematode-infective microsporidia. Molecular phylogenetic analysis grouped this new species with the recently identified nematode-infective parasites Enteropsectra and Pancytospora forming a monophyletic sister clade to Orthosomella in clade IV, which also includes human pathogens such as Enterocytozoon and Vittaforma. We believe that this newly discovered species and its host could have application as a new model in microsporidia–nematode association studies.

Keywords

Eukaryotic parasitesintracellular associationmicrosporidiosisnovel genusnovel speciesparasites of nematodes
Type
Research Article
Information
Parasitology , Volume 145 , Issue 14 , December 2018 , pp. 1853 - 1864
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Copyright
Copyright © Cambridge University Press 2018 

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Footnotes

*

Present address: Department of Infection Microbiology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.

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