Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-24T13:25:06.011Z Has data issue: false hasContentIssue false
  • English
  • Français

A neutral cysteine protease of Spirometra mansoni plerocercoid invoking an IgE response

Published online by Cambridge University Press:  01 March 1997

Y. KONG ,
S.-Y. KANG ,
S. H. KIM ,
Y.-B. CHUNG  and
S.-Y. CHO
Y. KONG
Affiliation:
Biomedical Research Center, Korea Institute of Science and Technology, Seoul 136-791, Korea
S.-Y. KANG
Affiliation:
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea
S. H. KIM
Affiliation:
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea
Y.-B. CHUNG
Affiliation:
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea
S.-Y. CHO
Affiliation:
Department of Parasitology, Catholic University of Korea School of Medicine, Seoul 137-701, Korea
Get access Rights & Permissions [Opens in a new window]

Abstract

When crude extracts of Spirometra mansoni plerocercoid (sparganum) were analysed by SDS–polyacrylamide gel electrophoresis (PAGE)/immunoblot using patients' sera, IgE antibodies reacted specifically with 21, 27 and 53 kDa proteins. The 21 and 27 kDa proteins have been previously characterized as cysteine proteases. In this study, the 53 kDa protein was confirmed, by immunoprecipitation, to induce a specific IgE response. The protein was purified by affinity chromatography using an IgG1 (κ2) type mAb. The protein was partially sensitive to peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase F (endo F) digestion. It exhibited an endoproteinase activity in a thiol-dependent manner preferentially degrading benzoyloxycarboxyl-phenylalanyl-arginyl-4-methoxy-β-naphthylamide (Z-phe-arg-MNA) of a panel of substrates tested. This endoprotease activity was maximal at pH 6·5 and in 0·1 M sodium phosphate. The proteolytic activity was inhibited by 10−5ML-trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane (E-64) and 1 mM iodoacetamide (IAA), and potentiated by dithiothreitol (DTT, 5 mM).

Keywords

Spirometra mansonisparganumsparganosisallergencysteine protease
Type
Research Article
Information
Parasitology , Volume 114 , Issue 3 , March 1997 , pp. 263 - 271
Copyright
1997 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)