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The mode of action of some benzimidazole drugs on Trichinella spiralis

Published online by Cambridge University Press:  06 April 2009

A. Criado Fornelio ,
F. Rodriguez Caabeiro  and
A. Jimenez Gonzalez
A. Criado Fornelio
Affiliation:
Department of Parasitology, Faculty of Pharmacy, University of Alcalá de Henares, Spain
F. Rodriguez Caabeiro
Affiliation:
Department of Parasitology, Faculty of Pharmacy, University of Alcalá de Henares, Spain
A. Jimenez Gonzalez
Affiliation:
Department of Parasitology, Faculty of Pharmacy, University of Alcalá de Henares, Spain
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Summary

In an attempt to find possible targets for benzimidazole action in muscle-stage larvae of Trichinella spiralis, the effects of mebendazole and thiabendazole were tested in vivo by oral treatment of infested mice and in vitro by including these anthelmintics in an adequate maintenance medium containing decapsulated larvae. The effects of the anthelmintics on succinate dehydrogenase and fumarate reductase activities, measured in the mitochondrial fraction obtained from the in vivo- or in vitro-treated larvae showed that only thiabendazole causes significant inhibition of fumarate reductase activity. On the other hand, measurements of free glucose, glycogen reserves and soluble protein in the treated larvae indicate that in vivo, mebendazole and thiabendazole clearly diminish free glucose levels, although in vitro only mebendazole produces the same diminution. Both the glycogen and protein contents of the larvae remained unchanged after treatment in vivo or in vitro. The importance of these findings with regard to a possible site of action for mebendazole and thiabendazole is discussed.

Type
Research Article
Information
Parasitology , Volume 95 , Issue 1 , August 1987 , pp. 61 - 70
Copyright
Copyright © Cambridge University Press 1987

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