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Isolation of Theileria parasites from African buffalo (Syncerus caffer) and characterization with anti-schizont monoclonal antibodies

Published online by Cambridge University Press:  06 April 2009

P. A. Conrad ,
D. A. Stagg ,
J. G. Grootenhuis ,
A. D. Irvin ,
J. Newson ,
R. E. G. Njamunggeh ,
P. B. Rossiter  and
A. S. Young
P. A. Conrad
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
D. A. Stagg
Affiliation:
Veterinary Research Department, Kenya Agricultural Research Institute (KARI), P.O. Box 32, Kikuyu, Kenya
J. G. Grootenhuis
Affiliation:
Wildlife Diseases Research Project, Veterinary Research Laboratory, P.O. Kabete, Kenya
A. D. Irvin
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
J. Newson
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
R. E. G. Njamunggeh
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
P. B. Rossiter
Affiliation:
Veterinary Research Department, Kenya Agricultural Research Institute (KARI), P.O. Box 32, Kikuyu, Kenya
A. S. Young
Affiliation:
Veterinary Research Department, Kenya Agricultural Research Institute (KARI), P.O. Box 32, Kikuyu, Kenya
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Extract

Antigenic differences between intra-lymphocytic theilerial parasites isolated from the blood of 18 African buffalo and grown in vitro were assessed with anti-schizont monoclonal antibodies (mAbs). There was marked antigenic diversity both between isolates from different buffalo and between isolates taken at different times from the same buffalo. Many of the isolates from both wild and captive buffalo appeared to consist of mixed parasite populations. Some isolates were found by limiting dilution cloning and mAb testing to contain at least 3 or 4 distinct populations of Theileria. Once cloned, Theileria-infected lymphoblastoid cell lines retained their mAb profiles during prolonged in vitro cultivation and, when recloned, the subclones had the same mAb profile as their parent clone. The implications of these results for further studies on buffalo-derived theilerial parasites are discussed.

Type
Research Article
Information
Parasitology , Volume 94 , Issue 3 , June 1987 , pp. 413 - 423
Copyright
Copyright © Cambridge University Press 1987

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