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In vitro activity of the F-6 fraction of oregano against Giardia intestinalis

Published online by Cambridge University Press:  06 February 2012

YADIRA RUFINO-GONZÁLEZ ,
MARTHA PONCE-MACOTELA ,
ANGÉLICA GONZÁLEZ-MACIEL ,
RAFAEL REYNOSO-ROBLES ,
MANUEL JIMÉNEZ-ESTRADA ,
ÁNGELES SÁNCHEZ-CONTRERAS  and
MARIO N. MARTÍNEZ-GORDILLO
YADIRA RUFINO-GONZÁLEZ
Affiliation:
Parasitología experimental del Instituto Nacional de Pediatría, Insurgentes Sur no. 3700-C, C. P. 04530, México, D. F.,México
MARTHA PONCE-MACOTELA
Affiliation:
Parasitología experimental del Instituto Nacional de Pediatría, Insurgentes Sur no. 3700-C, C. P. 04530, México, D. F.,México
ANGÉLICA GONZÁLEZ-MACIEL
Affiliation:
Microscopia electrónica del Instituto Nacional de Pediatría, Insurgentes Sur no. 3700-C, C. P. 04530, México, D. F., México
RAFAEL REYNOSO-ROBLES
Affiliation:
Microscopia electrónica del Instituto Nacional de Pediatría, Insurgentes Sur no. 3700-C, C. P. 04530, México, D. F., México
MANUEL JIMÉNEZ-ESTRADA
Affiliation:
Laboratorio de Productos Naturales, Instituto de Química de la UNAM, Circuito exterior, Ciudad Universitaria, C. P. 04510, México, D. F., México
ÁNGELES SÁNCHEZ-CONTRERAS
Affiliation:
Unidad Sureste del Centro de Investigación y Asistencia Tecnológica en Diseño del Estado de Jalisco, AC, Calle 30 No. 151, C. P. 97070, Mérida, Yucatán, México
MARIO N. MARTÍNEZ-GORDILLO*
Affiliation:
Parasitología experimental del Instituto Nacional de Pediatría, Insurgentes Sur no. 3700-C, C. P. 04530, México, D. F.,México
*
*Corresponding author: Parasitología experimental, Instituto Nacional de Pediatría, Insurgentes Sur No. 3700-C, Mexico, D. F. CP 04530, Mexico. Tel: +52 55 10840900 ext. 1454. Fax +52 55 1084 3884. E-mail [email protected]
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Summary

Giardiosis is a neglected parasitic disease that produces diarrhoea and different degrees of malabsorption in humans and animals. Its treatment is based on derivatives of 5-nitroimidazoles, benzimidazoles, nitrofuranes, acridine and nitrotiazoles. These drugs produce undesirable secondary effects, ranging from a metallic taste in the mouth to genetic damage and the selection of resistant strains; therefore, it is necessary to develop new therapeutic alternatives. We demonstrated that a 2-h treatment with 2·87 μg ml−1 of fraction 6 of Lippia graveolens (F-6) was sufficient to kill half of an experimental Giardia intestinalis (Syn. G. duodenalis, G. lamblia) population, based on the reduction of MTT-tetrazolium salt levels. F-6 breaks the nuclear envelope and injures the ventral suckling disc. The major compounds of F-6 were characterized as naringenin, thymol, pinocembrin and traces of compounds not yet identified. The results suggest that Lippia is a potential source to obtain compounds with anti-Giardia activity. This knowledge is an important starting point to develop new anti-giardial drugs. Future studies will be required to establish the efficacy of F-6 in vivo using an animal model.

Keywords

Giardia intestinalisLippia extractsalternative treatmentsnaringeninultrastructure
Type
Research Article
Information
Parasitology , Volume 139 , Issue 4 , April 2012 , pp. 434 - 440
Copyright
Copyright © Cambridge University Press 2012

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