Hostname: page-component-586b7cd67f-g8jcs Total loading time: 0 Render date: 2024-11-30T16:08:32.509Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of anti-thymocyte serum on the eosinophil and lysophospholipase responses in mice infected with Trichinella spiralis

Published online by Cambridge University Press:  06 April 2009

K. Adewusi  and
A. J. Goven
K. Adewusi
Affiliation:
Department of Biological Sciences, North Texas State University, Denton, Texas 76203
A. J. Goven
Affiliation:
Department of Biological Sciences, North Texas State University, Denton, Texas 76203
Get access Rights & Permissions [Opens in a new window]

Summary

Anti-thymocyte serum (ATS) was administered to CD-1 mice infected with 100 Trichinella spiralis larvae and its effects on intestinal lysophospholipase (EC 3.1.1.5) activity, peripheral blood lymphocytes and bone marrow, peripheral blood and intestinal eosinophilia were assayed in the same experimental animal. The ATS caused a significant suppression of both the tissue lysophospholipase response and eosinophilia, in all three compartments, when compared to the values found in infected mice that were either treated with normal rabbit serum (NRS) or untreated. The suppressed eosinophil response and reduced lysophospholipase activity demonstrated a close temporal relation throughout the experiment. These findings support the hypothesis that helminth parasite-induced eosinophilia is the cause of increased lysophospholipase activity present in parasitized tissue and that the responses are thymus cell dependent.

Type
Research Article
Information
Parasitology , Volume 94 , Issue 1 , February 1987 , pp. 115 - 122
Copyright
Copyright © Cambridge University Press 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ackerman, S. J., Durack, D. T. & Gleich, G. J. (1982). Eosinophil effector mechanisms in health and disease. In Advances in Host Defense Mechanisms, vol. 1 (ed. Gallin, J. I. and Fauci, A. S.), pp. 269–93. New York: Raven Press.Google Scholar
Castro, G. A. & Fairbairn, D. (1969). Carbohydrates and lipids in Trichinella spiralis and their utilization in vitro. Journal of Parasitology 55, 51–8.CrossRefGoogle ScholarPubMed
Colley, D. G. (1980). Lymphocyte products. In The Eosinophil in Health and Disease (ed. Mahmoud, A. A. F., Austin, K. F. and Simon, A. S.), pp. 293–309. New York: Grune & Stratten.Google Scholar
Davis, R. C., Cooperband, S. R. & Mannick, J. A. (1970). Effective and ineffective antilymphocyte sera correlation of in vitro test with potency of early antisera (34584). Proceedings of the Society for Experimental Biology and Medicine 133, 874–7.Google Scholar
Dessein, A. J. & David, J. R. (1982). The eosinophil in parasitic diseases. In Advances in Host Defense Mechanisms, vol. 1 (ed. Gallin, J. I. and Fauci, A. S.), pp. 243–68. New York: Raven Press.Google Scholar
Goulson, H. T., Ottolenghi, A. & Larsh, J. E. jr. (1981). Phospholipase B in nonsensitized and sensitized rats after challenge with Strongyloides ratti. American Journal of Tropical Medicine and Hygiene 30, 350–7.Google Scholar
Goven, A. J. (1979 a). The phospholipase B content of the intestines of rats infected with varied larvae doses of Nippostrongylus brasiliensis. International Journal for Parasitology 9, 193–8.Google Scholar
Goven, A. J. (1979 b). The phospholipase B content of the intestines of sensitized rats challenged with varied doses of Nippostrongylus brasiliensis. International Journal for Parasitology 9, 345–9.Google Scholar
Goven, A. J. (1983). Effect of anti-eosinophil serum on phospholipase B activity in mice infected with Trichinella spiralis. Journal of Parasitology 69, 88–90.Google Scholar
Goven, A. J. & Moore, G. W. (1980). Phospholipase B activity in congenitally athymic (nude) mice infected with Trichinella spiralis. Zeitschrift für Parasitenkunde 61, 265–9.CrossRefGoogle Scholar
Kazura, J. W. & Aikawa, M. (1980). Host defense mechanisms against Trichinella spiralis infection in the mouse: Eosinophil-mediated destruction of newborn larvae in vitro. Journal of Immunology 124, 355–61.CrossRefGoogle ScholarPubMed
Kirk, C. J., Peel, R. N., James, K. R. & Kershaw, Y. (1975). Basic Medical Laboratory Technology. New York: John Wiley and Sons.Google Scholar
Larsh, J. E. Jr & Kent, D. E. (1949). The effects of alcohol on natural and acquired immunity of mice to infection with Trichinella spiralis. Journal of Parasitology 35, 45–53.Google Scholar
Larsh, J. E. Jr, Ottolenghi, A. & Weatherly, N. F. (1974). Trichinella spiralis: Phospholipase in challenged mice and rats. Experimental Parasitology 36, 299–306.CrossRefGoogle ScholarPubMed
Larsh, J. E. Jr, Ottolenghi, A. & Weatherly, N. F. (1975). Trichinella spiralis: Phospholipase in sensitized mice after challenge. Experimental Parasitology 37, 233–8.Google Scholar
Laubach, H. R. (1982). Intestinal lysophospholipase and eosinophil responses to Macracanthorhynchus hirudinaceus (Acanthocephala). Journal of Parasitology 68, 962–3.Google Scholar
Ogilvie, B. M., Askenase, P. W. & Rose, M. E. (1980). Basophils and eosinophils in three strains of rats and in athymic (nude) rats following infection with the nematode Nippostrongylus brasiliensis or Trichinella spiralis. Immunology 39, 385–9.Google ScholarPubMed
Ottolenghi, A. (1973). High phospholipase content of intestines of mice infected with Hymenolepis nana. Lipids 8, 426–8.CrossRefGoogle ScholarPubMed
Ottolenghi, A., Kocan, A. A., Weatherly, N. F. & Larsh, J. E. jr. (1975). Nippostrongylus brasiliensis: Phospholipase in nonsensitized and sensitized rats after challenge. Experimental Parasitology 38, 96–104.CrossRefGoogle ScholarPubMed
Ottolenghi, A., Kocan, A. A., Weatherly, N. F. & Larsh, J. E. jr. (1977). Angiostrongylus cantonensis: Phospholipase in nonsensitized and sensitized rats after challenge. Infection and Immunity 15, 13–18.Google Scholar
Ottolenghi, A., Pickett, J. P. & Green, W. B. (1967). Histochemical demonstration of phospholipase B (lysolecithinase) activity in rat tissues. Journal of Histochemistry and Cytochemistry 14, 907–14.Google Scholar
Ruitenburg, E. J., Elgersma, A., Kruizinga, W. & Leenstra, F. (1977). Trichinella spiralis infection in congenitally athymic (nude) mice. Parasitology, serological and haematological studies with observations in intestinal pathology. Immunology 33, 581–7.Google Scholar
Weatherly, N. F. (1970). Increased survival of Swiss mice given sublethal infections of Trichinella spiralis. Journal of Parasitology 56, 748–53.CrossRefGoogle ScholarPubMed
Weller, P. J. & Goetzl, E. J. (1979). The regulatory and effector roles of eosinophils. In Advances in Immunology, vol. 27, (ed. Kunkel, H. G. and Dixon, F. J.), pp. 339–71. New York: Academic Press.Google Scholar
Wilkes, S. D. & Goven, A. J. (1984). Tissue eosinophil numbers and phospholipase B activity in mice infected with Trichinella spiralis. International Journal for Parasitology 14, 479–82.Google Scholar