Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2024-12-18T19:42:05.499Z Has data issue: false hasContentIssue false
  • English
  • Français

Comparative sequence analysis of 5·8S rRNA genes and internal transcribed spacer (ITS) regions of trichomonadid protozoa

Published online by Cambridge University Press:  01 August 1997

R. S. J. FELLEISEN
R. S. J. FELLEISEN
Affiliation:
Institute of Parasitology, University of Bern, Länggass-Strasse 122, CH-3012 Bern, Switzerland
Get access Rights & Permissions [Opens in a new window]

Abstract

The taxonomic situation in the genus Tritrichomonas is the subject of controversial discussion: potentially T. foetus and T. suis, the tritrichomonads from cattle and swine, respectively, could belong to the same species. In order to shed some light on this question, a molecular biological analysis was performed. The 5·8S rRNA gene and the flanking internal transcribed spacer regions (ITS1 and ITS2) of 12 different isolates of 3 Tritrichomonas species T. foetus, T. suis and T. mobilensis were enzymatically amplified by PCR and subcloned. Also, the corresponding regions of the trichomonads Trichomonas vaginalis, T. tenax, T. gallinae and Pentatrichomonas hominis were included in this study. Sequence analysis of cloned fragments was used to compare the parasite isolates. The genus Tritrichomonas exhibited an extremely high degree of homogeneity. All T. foetus and T. suis isolates had identical sequences, and only 1 substitution was found in the ITS2 region of T. mobilensis. In contrast, the genus Trichomonas shared more diversity. The results obtained in this study support a possible future revision of the taxonomic classification of tritrichomonads.

Keywords

TrichomonasTritrichomonasPentatrichomonas5·8S rRNA geneinternal transcribed spacerpolymerase chain reaction (PCR)
Type
Research Article
Information
Parasitology , Volume 115 , Issue 2 , August 1997 , pp. 111 - 119
Copyright
1997 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)