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The capacity of the fungus Duddingtonia flagrans to prevent strongyle infections in foals on pasture

Published online by Cambridge University Press:  06 April 2009

M. Larsen ,
P. Nansen ,
C. Grøndahl ,
S. M. Thamsborg ,
J. Grønvold ,
J. Wolstrup ,
S. A. Henriksen  and
J. Monrad
M. Larsen*
Affiliation:
Danish Centre for Experimental Parasitology, Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
P. Nansen
Affiliation:
Danish Centre for Experimental Parasitology, Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
C. Grøndahl
Affiliation:
Department of Anatomy and Physiology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
S. M. Thamsborg
Affiliation:
Danish Centre for Experimental Parasitology, Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark Department of Clinical Studies, Large Animal Medicine, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
J. Grønvold
Affiliation:
Danish Centre for Experimental Parasitology, Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark Section of Zoology and Molecular Biology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1958 Frederiksberg C, Denmark
J. Wolstrup
Affiliation:
Danish Centre for Experimental Parasitology, Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark Microbiology Section, Department of Ecology and Molecular Biology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1958 Frederiksberg C, Denmark
S. A. Henriksen
Affiliation:
Danish Centre for Experimental Parasitology, Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark National Veterinary Laboratory, 27 Bülowsvej, DK-1790 Copenhagen V, Denmark
J. Monrad
Affiliation:
Danish Centre for Experimental Parasitology, Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
*
*Corresponding author. Tel: + 45 35 282794. Fax: + 45 35 282774. E-mail: [email protected]
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Summary

A field trial was conducted to evaluate the potential of the nematode-destroying fungus Duddingtonia flagrans to control free-living stages of horse strongyles. In late Spring 2 groups of horses (yearlings) with mixed infections of strongyles were allowed to contaminate 2 equal-sized pastures. One of the groups (F) received a daily dose of D. flagrans mixed in a feed supplement, while the other (C) received a similar amount of supplement without fungus. During a 3-month contamination period strongyle egg counts in faeces and number of infective strongyle larvae harvested from faecal cultures were determined. Grass samples were collected fortnightly. After the contamination period the yearlings were removed and 2 groups of young tracer foals (TF and TC) grazed the fungus and control pastures respectively for 4 weeks, housed for another 15 weeks and then killed to determine their worm burdens. The number of larvae in cultures from group TF was significantly lower than that in TC and herbage infectivity was reduced to a very low level on the pasture grazed by horses fed fungi. The number of Strongylus vulgaris and Strongylus edentatus larvae was also significantly lowered in group TF. Cyathostome larvae recovered from the mucosa of the ventral and dorsal colon and from the caecum were significantly lowered in group TF foals. Also, the number of strongyles found in the gut contents of group TF foals were significantly reduced in the dorsal colon, but numbers of worms in the ventral colon and in the caecum were similar to those of the controls.

Keywords

biological controlDuddingtonia flagransstrongyleshorsesfield trial
Type
Research Article
Information
Parasitology , Volume 113 , Issue 1 , July 1996 , pp. 1 - 6
Copyright
Copyright © Cambridge University Press 1996

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References

REFERENCES

Bird, J. & Herd, R. P. (1995). In vitro assessment of two species of nematophagous fungi (Arthrobotrys oligospora and Arthrobotrys flagrans) to control the development of infective cyathostome larvae from naturally infected horses. Veterinary Parasitology 56 181–7.CrossRefGoogle ScholarPubMed
Charles, T. P., Rodrigues, M. L. A. & Santos, C. P. (1995). Reducao do número de larvas de Cyathostominae em fezes de eqüinos tratadas com conídios de Arthrobotrys oligospora. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia 47, 87–9.Google Scholar
Eysker, M., Jansen, J., Kooyman, F. N. J., Mirck, M. H. & Wensing, T. H. (1986). Comparison of two control systems for cyathostome infections in the horse and further aspects of the epidemiology of these infections. Veterinary Parasitology 22, 105–12.CrossRefGoogle ScholarPubMed
Grønvold, J., Wolstrup, J., Larsen, M., Henriksen, S. A. & Nansen, P. (1993 a). Biological control of Ostertagia ostertagi by feeding selected nematodetrapping fungi to calves. Journal of Helminthology 67, 31–6.CrossRefGoogle ScholarPubMed
Grønvold, J., Wolstrup, J., Nansen, P., Henriksen, Sv.Aa., Larsen, M. & Bresciani, J. (1993 b). Biological control of nematode parasites in cattle with nematodetrapping fungi – a survey of Danish studies. Veterinary Parasitology 48, 311–25.CrossRefGoogle ScholarPubMed
Herd, R. P. (1993). Control strategies for ruminant and equine parasites to counter resistance, encystment, and ecotoxicity in the USA. Veterinary Parasitology 48, 327–36.CrossRefGoogle ScholarPubMed
Klei, T. R., Chapman, M. R., French, D. D. & Taylor, H. W. (1993). Evaluation of ivermectin at an elevated dose against encysted equine cyathostome larvae. Veterinary Parasitology 47, 99–106.CrossRefGoogle ScholarPubMed
Larsen, M., Wolstrup, J., Henriksen, S. A., Dackman, C., Grønvold, J. & Nansen, P. (1991). In vitro stress selection of nematophagous fungi for biocontrol of parasitic nematodes in ruminants. Journal of Helminthology 65, 193–200.CrossRefGoogle ScholarPubMed
Larsen, M., Nansen, P., Wolstrup, J., Grønvold, J., Henriksen, S. A. & Zorn, A. (1995 a). Biological control of trichostrongyles in calves by the fungus Duddingtonia flagrans fed to animals under natural grazing conditions, Veterinary Parasitology 60, 321–30.CrossRefGoogle ScholarPubMed
Larsen, M., Nansen, P., Henriksen, S. A., Wolstrup, J., Grønvold, j., Zorn, A. & Wedø, E. (1995 b). Predacious activity of the nematode-trapping fungus Duddingtonia flagrans against cyathostome larvae in faeces after passage through the gastrointestinal tract of horses. Veterinary Parasitology 60, 315–20.CrossRefGoogle ScholarPubMed
Love, S. & Duncan, J. L. (1992). The development of naturally acquired cyathostome infection in ponies. Veterinary Parasitology 44, 127–42.CrossRefGoogle ScholarPubMed
Nansen, P., Grønvold, J., Henriksen, S. A. & Wolstrup, J. (1988). Interactions between the predacious fungus Arthrobotrys oligospora and third-stage larvae of a series of animal parasitic nematodes. Veterinary Parasitology 26, 329–37.CrossRefGoogle ScholarPubMed
Nansen, P., Larsen, M., Grønvold, J., Wolstrup, J., Zorn, A. & Henriksen, S.Aa. (1995). Prevention of clinical trichostrongylidosis in calves by strategic feeding with the predacious fungus Duddingtonia flagrans. Parasitology Research 81, 371–4.CrossRefGoogle ScholarPubMed
Reinemeyer, C. R. & Herd, R. P. (1986). Anatomic distribution of encysted cyathostome larvae in the horse. American Journal of Veterinary Research 47, 510–13.Google ScholarPubMed
Slotved, H.-C., Barnes, E. H., Bjørn, H., Christensen, C. M., Eriksen, L., Roepstorff, A. & Nansen, P. (1996). Recovery of Oesophagostomum dentatum from pigs by isolation of parasites migrating from large intestinal contents embedded in agar-gel. Veterinary Parasitology (in the press).CrossRefGoogle ScholarPubMed
Statistical Analysis Systems (1990). SAS/STAT Users Guide, Version 6, 4th Edition, SAS Institute Inc.. Cary, NC, USA.Google Scholar
Wolstrup, J., Grønvold, J., Henriksen, Sv.Aa., Nansen, P., Larsen, M., Bøgh, H. O. & Ilsøe, B. (1994). An attempt to implement the nematode-trapping fungus Duddingtonia flagrans in biological control of free-living stages of trichostrongyles in first year grazing calves. Journal of Helminthology 68, 175–80.CrossRefGoogle Scholar