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The activation of cystacanths of Polymorphus minutus (Acanthocephala) in vitro

Published online by Cambridge University Press:  06 April 2009

Ann M. Lackie
Ann M. Lackie
Affiliation:
Molteno Institute, University of Cambridge, Downing Street, Cambridge CB2 3EE
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Extract

The factors responsible for stimulating activation of cystacanths of Polymorphus minutus in vitro have been investigated. Under the conditions used, the optimum temperature for activation was found to be 42–44 °C, at a pH of 7·0. A marked enhancement of activation rate was observed after preincubation in a 1 mM solution of sodium taurocholate and in solutions of sodium cholate, sodium glycocholate or sodium deoxycholate. This apparent effect of bile salts has been compared with that of other detergents, and a significant enhancement of activation rate above control was observed with saponin and sodium fusidate. Trypsin did not affect the rate of activation, and some inhibition occurred with lipase. The osmotic pressure of the surrounding medium has an effect on activation, the rate of which varies inversely with osmolarity. The conditions for activation in vitro are compared with those of the physicochemical environment in vivo.

Type
Research Article
Information
Parasitology , Volume 68 , Issue 2 , April 1974 , pp. 135 - 146
Copyright
Copyright © Cambridge University Press 1974

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