In situ conservation is central to contemporary global biodiversity protection and is the predominant emphasis of international regulation and funding strategies. Ex situ approaches, in contrast, have been relegated to a subsidiary role and their direct contributions to conservation have been limited. We draw on a variety of sources to make the case for an enhanced role for ex situ conservation. We note the advances occurring within institutions specializing in ex situ conservation and stress that, although much remains to be done, many constraints are being addressed. We argue that the evidence of increasing extinction rates, exacerbated by climate change, challenges the wisdom of a heavy dependence on in situ strategies and necessitates increased development of ex situ approaches. A number of different techniques that enable species and their habitats to survive should now be explored. These could build on the experience of management systems that have already demonstrated the effective integration of in situ and ex situ techniques and hybrid approaches. For organizations specializing in ex situ conservation to become more effective, however, they will require tangible support from the institutions of global biodiversity governance. Resistance is anticipated because in situ conservation is entrenched through powerful groups and organizations that exert influence on global conservation policy and facilitate the flow of funding. The chasm that has traditionally divided in situ and ex situ approaches may diminish as approaches are combined. Moreover, the relentless loss of the ‘wild’ may soon render the in situ / ex situ distinction misleading, or even obsolete.

We explore the potential for payments for ecosystem services (PES) to reconcile conservation and development goals, using a case study of an experimental PES intervention around the Nyungwe National Park in Rwanda. The scheme involves the purchase of biodiversity conservation services from local communities in four selected locations. Although a portion of the payment is awarded at the household level, it is the collective action of the community that determines the level of the payment. Contracts are negotiated annually and include performance indicators within each participating community. We examine the ability of PES to achieve conservation and development objectives, through three sub-questions: Is the PES scheme effective? Is it legitimate and fair? Is it equitable? Our findings indicate that the relationship between these evaluation criteria is complex, with both trade-offs and synergies. In this case study the effectiveness of PES is dependent on the equitable distribution of the payment, participants’ belief and acceptance of the service being paid for, institutional histories that aid in the establishment of legitimacy and fairness, and the complementary nature of PES to more conventional enforcement methods.

Sustaining the regular flow of water from mountain forests is important for downstream stakeholders in seasonally dry tropical countries, and a watershed payment for ecosystem services (PES) scheme that links rural ecosystem service providers to urban water users through economic transfers may help to maintain water supply and forest habitat. A CARE/WWF project in the Uluguru Mountains of Tanzania has established a pilot watershed PES scheme. We trace the development of this scheme and outline its initial impacts. Memoranda of Understanding between companies in Dar es Salaam (the downstream water users) and farmers in the mountains provide the framework to deliver tangible financial benefits to local people, help change patterns of land use and potentially improve water quality. A number of lessons learned from this project are relevant for similar schemes elsewhere in the region. Overcoming the various challenges is essential for expanding the current pilot project to a scale where it delivers measurable changes in water quality for downstream users.

A key question in the literature on payments for ecosystem services (PES) is how payments incentivize conservation action and, in particular, how they interact with other motivations, including motivations for environmental stewardship. Related to this question are concerns about the temporal sustainability of PES: what happens when payments cease and whether a ‘no pay, no care’ environmental ethic is fostered. I present empirical research from a case study in western Uganda, where forest-adjacent communities are paid in exchange for planting trees on private lands, for carbon sequestration. The study demonstrates the range of values people have for trees in the landscape and the range of motivations for participating in PES schemes. However, the analysis shows that payments are clearly the main motivation for involvement, except in one area where people are more motivated by aesthetic and existence values for trees. Given the widespread importance of money in motivating involvement, I investigate the profitability of participation over time. This profitability analysis, in combination with qualitative data on perceptions of, and plans for, the future, contributes to understanding the temporal sustainability of PES. I draw on various strands of evidence to argue that the way participants prioritize payments may constitute a threat to the long-term maintenance of PES activities, particularly in situations such as in this case study, in which there is a mismatch between payments and contract length.

Payments for ecosystem services (PES) are emerging worldwide as important mechanisms to align investments in human and natural well-being. PES projects are often defined as voluntary transactions where well-defined environmental/ecosystem services (or land uses likely to secure those services) are bought by a minimum of one service buyer, from a minimum of one service provider, if and only if the service provider continuously secures service provision (conditionality). Further criteria of PES include limiting additional objectives and ensuring that payments reward behaviour that would otherwise not occur (additionality). Together these best practices for PES are increasingly accepted as the most efficient means to achieve desired outcomes and are guiding funding for PES projects. We used a series of water funds (watershed-oriented PES projects based on a trust fund model) to examine how theoretical best practices could inform and improve practice and also how theory could learn from practical efforts. We conclude that thoughtful consideration is required when evaluating the promise of a PES approach against a theoretical ideal. We found that requiring conditionality may limit the use of creative finance mechanisms such as trust funds that can provide long-term benefits for conservation and human well-being, and that requiring additionality can exclude benefits from social diffusion and result in the inefficient targeting of PES funds. Finally, public–private partnerships in water funds lead to multiple additional/side objectives but partnerships are likely to lower transaction costs and provide transparent, long-term landscape-scale watershed management.

Migratory pathways in North American prairies are critical for sustaining endemic biodiversity. Fragmentation and loss of habitat by an encroaching human footprint has extirpated and severely truncated formerly large movements by prairie wildlife populations. Greater sage-grouse Centrocercus urophasianus, a Near Threatened landscape species requiring vast tracts of intact sagebrush Artemisia spp., exhibit varied migratory strategies across their range in response to the spatial composition of available habitats. We unexpectedly documented the longest migratory event ever observed in sage-grouse (> 120 km one way) in 2007–2009 while studying demography of a population at the north-east edge of their range. Movements that encompassed 6,687 km2 included individuals using distinct spring and summer ranges and then freely intermixing on the winter range in what is probably an obligate, annual event. The fate of greater sage-grouse in Canada is in part dependent on habitat conservation in the USA because this population spans an international border. Expanding agricultural tillage and development of oil and gas fields threaten to sever connectivity for this imperilled population. Science can help delineate high priority conservation areas but the fate of landscapes ultimately depends on international partnerships implementing conservation at scales relevant to prairie wildlife.

The coastal waters of Baja California Sur, Mexico, include some of the most important foraging grounds of the East Pacific green turtle Chelonia mydas. However, they are also important fishing grounds for artisanal fleets, leading potentially to high levels of bycatch mortality. We studied the impact of a small-scale gill-net fishery at San Ignacio lagoon, north-west Mexico, an important green turtle feeding ground. We conducted mortality censuses and interviewed local fishers to estimate total bycatch mortality at the lagoon. We also used marked drifters and carcasses to estimate stranding probabilities of turtles taken as bycatch. During 2006–2009 we found 262 dead turtles; 96% of the mortality occurred in May–August corresponding to the fishing season for halibut Paralichthys californicus and guitar-fish (Rhinobatus sp.). Stranding probability estimated from drifters was 0.062 (95% confidence interval, CI, 0.035–0.094), yielding a minimum mortality of 3,516 turtles during 2006–2008 (95% CI 2,364–6,057) or 1,172 animals per year. This is probably an underestimate of real mortality as the drifters have higher stranding probabilities than carcasses and most of the nets were set in the lower lagoon where carcasses rarely strand. Interviews with local fishers yielded a similar estimate of 1,087 (95% CI 901–1,286) dead turtles per year. This study is emblematic of the impact of artisanal fleets on marine turtles caused by overlap of fishing and turtle feeding areas. In 2009 strandings declined by > 97%, resulting from a change in fishing practices because of increased vigilance by enforcement authorities, underscoring the importance of law enforcement to protect threatened species.

Population monitoring is a vital tool for conservation management and for testing hypotheses about population trends in changing environments. Darwin’s finches on Santa Cruz Island in the Galápagos archipelago have experienced habitat alteration because of human activity, introduced predators, parasites and disease. We used point counts to conduct systematic quantitative surveys of Darwin’s finches and other land birds between 1997 and 2010. The temporal analysis revealed that six of the nine species investigated declined significantly and that this decline was most pronounced at higher elevations in humid native forest and agricultural areas; the highland areas have been most affected by introduced species or direct human impact. Five of the six declining species are insectivorous, which suggests that changes in insect abundance or insect availability are a critical factor in the declines. Further study is required to test this idea. Other factors including habitat alteration and introduced parasites or pathogens may be contributing to the observed declines.

We report a range-wide status assessment of a key Neotropical ecosystem architect, the white-lipped peccary Tayassu pecari, categorized as Near Threatened on the IUCN Red List, using published information and unpublished data from 41 scientists in 15 range countries. We estimate that the white-lipped peccary has been extirpated in 21% of its historical range over the last 100 years, with reduced abundance and a low to medium probability of long-term survival in another 48% of its current range. We found major range declines in Argentina, Paraguay, southern Brazil, Colombia, Venezuela, north-east Brazil, Mexico and Costa Rica. This species is particularly at risk in more xeric ecosystems, especially the caatinga, cerrado and pampas. Hunting and habitat destruction are the most severe threats, although there are also unexplained sudden die-offs suggestive of disease. We evaluate our results in light of this species’ important interspecific interactions and its role as an ecosystem architect. One of our recommendations is that conservation efforts should focus on landscape conservation of large, continuous and ecologically intact areas containing a mosaic of different habitat types.

The guanaco Lama guanicoe was the only large native herbivore widely distributed across Patagonia until the introduction of domestic sheep Ovis aries. Guanacos have declined because of competition with sheep for forage, high hunting pressure and habitat degradation. Península Valdés is a protected area where sheep ranching is the predominant activity. A ranch formerly dedicated to sheep production was converted into a private wildlife reserve, from which all the sheep were removed in 2005. We studied changes in guanaco abundance inside and outside the reserve after sheep removal, and also plant cover of various vegetation types. We found that guanaco abundance was higher inside than outside the reserve, and increased by three-fold within 3 years. Total plant cover and grass cover were higher inside than outside the reserve. Our results showed that guanacos reacted rapidly to changes in management, and suggest that even at a high density guanacos would not be as damaging to the vegetation as sheep. Although management changes resulted in significant changes in guanaco abundance locally, the size of a protected area influences the persistence of wild populations. A large herbivore such as the guanaco needs to be managed across large areas. We believe it is necessary to implement a management plan for Península Valdés that allows for the coexistence of sustainable livestock production and healthy wildlife populations.

There is evidence for the ecological extinction of the native prey of the puma Puma concolor in north-western Argentine Patagonia. In this study we examine whether this is also the case in southern Patagonia. From 2004 to 2007 we examined the puma’s diet in three protected areas and two sheep ranches in Santa Cruz province. A total of 282 puma scats were analysed. In two of the protected areas and in the ranches 60–74% of the puma’s diet was native prey. Prey species were primarily guanaco Lama guanicoe, followed by Patagonian mara Dolichotis patagonum, lesser rhea Pterocnemia pennata pennata, Patagonian pichi Zaedyus pichiy and Magellanic penguin Spheniscus magellanicus. In the third protected area the main prey was the European hare Lepus europaeus. Our results show a clear difference in the diet of the puma in southern compared to north-western Patagonia. Large native herbivores (i.e. guanaco and lesser rhea) maintain their role as the main prey species for the puma in southern Patagonia. We suggest, therefore, that native prey could be restored to those areas of Argentine Patagonia, such as the north-west, where they are currently ecologically extinct. Facilitating native species recovery and/or restoration and applying more rigorous controls to prevent the introduction of potential alien prey species of the puma both, within and outside protected areas, needs to be evaluated as a regional strategy.

The great bustard Otis tarda became extinct in the UK during the 19th century due to a combination of factors, including hunting, egg collection and changes in agriculture. In 2003 a 10-year licence was granted to begin a trial to reintroduce the species back to the UK. Here we report on the first 5 years of the trial and assess the progress made towards establishing a founder population. From April 2004 to September 2009 a total of 102 great bustard chicks were imported from Russia and 86 released on Salisbury Plain. Monitoring showed that post-release survival was 18% in the first year following release, and that mortality of released bustards was mainly attributable to predation and collisions. Estimated adult survival was 74%, although the sample size was small. All known surviving great bustards are faithful to the surroundings of the release site, returning throughout the year. A lek has been established where males have been observed displaying to females. The first nesting attempt was in 2007, and in 2009 two females aged 3 and 4 years successfully nested, fledging one chick each. Models incorporating the new demographic estimates suggest that at the end of the 10-year trial period the project can expect to have 8–26 adults as a founder population.

In recent decades there has been a marked decline in most ortolan bunting Emberiza hortulana populations in temperate Europe, with many regional populations now extinct or on the brink of extinction. In contrast, Mediterranean and, as far as we know, eastern European popula-tions seem to have remained relatively stable. The causes of decline remain unclear but include: habitat loss and degradation, and related reduction in prey availability; climate change on the breeding grounds; altered population dynamics; illegal captures during migration; and environmental change in wintering areas. We review the current knowledge of the biology of the ortolan bunting and discuss the proposed causes of decline in relation to the different population trends in temperate and Mediterranean Europe. We suggest new avenues of research to identify the factors limiting ortolan bunting populations. The main evidence-based conservation measure that is likely to enhance habitat quality is the creation of patches of bare ground to produce sparsely vegetated foraging grounds in invertebrate-rich grassy habitats close to breeding areas.