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Identification of products formed by a fructan [ratio ] fructan fructosyltransferase activity from Lolium rigidum

Published online by Cambridge University Press:  01 February 1997

J. A. St. JOHN ,
I. M. SIMS ,
G. D. BONNETT  and
R. J. SIMPSON
J. A. St. JOHN
Affiliation:
Department of Agriculture and Resource Management, The University of Melbourne, Parkville, Victoria, 3052, Australia
I. M. SIMS
Affiliation:
Department of Botany, Plant Cell Biology Research Centre, The University of Melbourne, Parkville, Victoria, 3052, Australia
G. D. BONNETT
Affiliation:
CSIRO Division of Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia
R. J. SIMPSON
Affiliation:
CSIRO Division of Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia
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Abstract

The products formed by a fructan [ratio ] fructan fructosyltransferase (FFT) activity purified from Lolium rigidum Gaudin were identified after gas chromatography-mass spectrometry of partially methylated alditol acetates, electrospray ionization-mass spectrometry and reversed-phase high-performance liquid chromatography. The FFT activity synthesized oligofructans up to degree of polymerization (DP) 6, but did not synthesize fructans of DP > 6 even when assayed with (1,1,1)-kestopentaose for up to 10 h. The FFT activity when assayed with 1-kestose or 6G-kestose synthesized fructan with fructosyl residues almost exclusively linked by β-2,1-glycosidic linkages. When assayed with 1-kestose, the FFT activity synthesized tetrasaccharides and pentasaccharides with an internal glucosyl residue. The predominant tetrasaccharide was (1&6G)-kestotetraose and the predominant pentasaccharide was (1&6G,1)-kestopentaose. By comparison, tetrasaccharides and pentasaccharides extracted from L. rigidum also contained predominantly β-2,1-glycosidic linked fructans with an internal glucosyl residue. The only exception was that one of the pentasaccharides contained β-2,1- and β-2,6-glycosidic linked fructosyl residues. This pentasaccharide was not synthesized by the FFT activity. The role of this FFT activity in formation of oligofructans in L. rigidum is discussed.

Keywords

Lolium rigidumfructan [ratio ] fructan fructosyltransferaseoligofructanfructan synthesisfructan structure
Type
Research Article
Information
The New Phytologist , Volume 135 , Issue 2 , February 1997 , pp. 249 - 257
Copyright
Trustees of the New Phytologist 1997

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