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Isozyme evidence for two groups of Fusarium graminearum

Published online by Cambridge University Press:  01 July 2000

M. LÁDAY
Affiliation:
Plant Protection Institute, Hungarian Academy of Sciences, P.O. Box 102, H-1525 Budapest, Hungary
F. BAGI
Affiliation:
Faculty of Agriculture, Institute for Plant Protection, 21000 Novi Sad, Yugoslavia
A. MESTERHÁZY
Affiliation:
Cereal Research Non-Profit Company, P.O. Box 391, H-6701 Szeged, Hungary. E-mail: [email protected].
Á. SZÉCSI
Affiliation:
Plant Protection Institute, Hungarian Academy of Sciences, P.O. Box 102, H-1525 Budapest, Hungary
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Abstract

Cellulose-acetate electrophoresis (CAE) was used to investigate isozyme polymorphisms of 34 Group 1 and Group 2 isolates of Fusarium graminearum. Of the 26 enzyme systems screened, alkaline phosphatase, NADP-dependent glutamate dehydrogenase, peptidase D and phosphoglucomutase were found to be suitable for distinguishing members of Group 1 from members of Group 2. A great deal of similarity in the isozyme patterns among the isolates of a same group, independently of geographic origin, indicated that isolates within a given group are descendant from a same ancestral population. Significant differences in the isozyme patterns between the isolates of Group 1 and Group 2 indicated, however, that the two groups were of different ancestry. Group 1 isolates have since been named F. pseudograminearum. Due to the short run time, minimal need of equipment, small sample volume and staining requirements, CAE generally provides a rapid and accurate method for isozyme analysis of Fusarium species.

Type
Research Article
Copyright
© The British Mycological Society 2000

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