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How the Morphology of Osteocytes Contributes to their Mechanotransduction near Microdamage

Published online by Cambridge University Press:  11 June 2015

Elisa Budyn ,
Morad Bensidhoum ,
Patrick Tauc ,
Eric Deprez  and
Herve Petite
Elisa Budyn
Affiliation:
Department of Mechanical engineering, LMT Laboratory CNRS UMR 8535, Ecole Normale Superieure Cachan, 61 Avenue du President Wilson, 94230 Cachan, France; University of Illinois at Chicago, 842 West Taylor Street, Chicago, IL 60607, USA.
Morad Bensidhoum
Affiliation:
Department of Biology, B2OA Laboratory UMR CNRS 7052, University Paris Diderot, Avenue de Verdun, 75010 Paris, France.
Patrick Tauc
Affiliation:
Department of Biology, LBPA Laboratory CNRS UMR 8113, Ecole Normale Superieure Cachan, 61 Avenue du President Wilson, 94230 Cachan, France.
Eric Deprez
Affiliation:
Department of Biology, LBPA Laboratory CNRS UMR 8113, Ecole Normale Superieure Cachan, 61 Avenue du President Wilson, 94230 Cachan, France.
Herve Petite
Affiliation:
Department of Biology, B2OA Laboratory UMR CNRS 7052, University Paris Diderot, Avenue de Verdun, 75010 Paris, France.
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Abstract

A dual experimental and numerical top-down approach is applied to investigate the link between osteocyte morphology and mechanical perception of their environment at the progenitor and mature stages. The numerical model is based on explicit tissue morphology discretization to identify bone diffuse damage at the cellular scale. The in vitro experimental model presents a live allograft bone system where a patient progenitor or mature osteocytes were reseeded in fresh human donor cortical bone tissues subjected to mechanical loading. The live systems behaved mechanically as fresh bone and the cells spatially reorganized in vitro as in vivo. The system under mechanical load also showed an adaptation of the calcium membrane transport rate to the expected in vivo mechanical load detected by bone cells at different stages of differentiation.

Keywords

biologicalbonefracture
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1724: Symposium H – Micro/Nano Engineering and Devices for Molecular and Cellular Manipulation, Simulation and Analysis , 2014 , mrsf14-1724-h12-24
Copyright
Copyright © Materials Research Society 2015 

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References

REFERENCES

Nather, A, David, V, Teng, J W H, Lee, C W, and Pereira, B P. effect of autologous mesenchymal stem cells on biological healing of allografts in critical-sized tibial defects simulated in adult rabbits. Annals Academy of Medicine, 39(8):599–606 (2010).Google ScholarPubMed
Jonvaux, J., Hoc, T. and Budyn, E., Analysis of micro fracture in human Haversian cortical bone under compression, Int. J. Num. Meth. Biomed. Eng., 28(9):974–998 (2012).CrossRefGoogle ScholarPubMed
Budyn, E. and Hoc, T., Analysis of micro fracture in human Haversian cortical bone under transverse tension using extended physical imaging., Int. J. Num. Meth. Eng., 82(8):940–965 (2010).CrossRefGoogle Scholar
Wang, Y, McNamara, L M, Schafler, M B, and Weinbaum, S. Strain amplification and integrin based signaling in osteocytes. J. Musculoskelet. Neuronal Interact, 8(4):332–334 (2008).Google ScholarPubMed
Kitase, Y, Van Der Plas, A, Semeins, C M, Ajubi, N E, Frangos, J A, Nijweide, P J, and Burger, E H. Mechanical induction of pge2 in osteocytes blocks glucocorticoid-induced apoptosis through both the beta-catenin and pka pathways. Journal of Bone Mineral Research, 25(12):2657–2668 (2010).CrossRefGoogle ScholarPubMed
Sun, X, McLamore, E, Kishore, V, Slipchenko, M, Porterfield, D M, and Akkus, O. Mechanical stretch induced calcium efflux from bone matrix stimulates osteoblasts. Bone, 50:581–591 (2012).CrossRefGoogle ScholarPubMed
Budyn, E, Tauc, P, Bensidhoum, M, Petite, H, and Deprez, E. Back to life: fresh osteocytes spreading their processes for optimum mechanotransduction near micro damage in dead bone. Medical Engineering Centres Annual Meeting and Bioengineering14, edited by Imperial College London, (MECbioeng14 Imperial College London, England, UK, 2014) ISBN 978-0-9930390-0-3, pp. 63.Google Scholar