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Structural Characteristics and Diffusion Coefficient of Alginate Hydrogels Used for Cell Based Drug Delivery

Published online by Cambridge University Press:  22 May 2018

Avid Najdahmadi ,
Jonathan RT Lakey  and
Elliot Botvinick
Avid Najdahmadi*
Affiliation:
Department of Chemical Engineering and Materials Science, University of California at Irvine, Irvine, USA.
Jonathan RT Lakey
Affiliation:
Department of Surgery, University of California at Irvine, Irvine, USA
Elliot Botvinick
Affiliation:
Department of Chemical Engineering and Materials Science, University of California at Irvine, Irvine, USA. Department of Biomedical Engineering, University of California at Irvine, Irvine, USA. Department of Surgery, University of California at Irvine, Irvine, USA
*
*(Email: [email protected])
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Abstract

Alginate is a hydrogel polymer commonly used in multiple drug delivery and cellular tissue transplantation applications. Tunability, gel formation, and stabilization properties of this biopolymer contributes to a better controlled and prolonged release of encapsulated drugs as well as the ability to provide immunoisolation to transplanted cells. One commonly used application of this biopolymer is pancreatic islet transplantation, as a promising approach of providing insulin to type 1 diabetics. The encapsulant alginate provides passage to nutrients, glucose and oxygen and allows the insulin to diffuse while blocking immunoglobulins. In this study, a hydrogel encapsulator is designed and used to fabricate spherical alginate microcapsules. These capsules are then incubated in either a calcium chloride solution typical used to polymerize alginate or a physiological media formulated to mimic in vivo conditions. The diffusion of different molecular weight particles tagged with spectrally distinct fluorescent molecules into the microspheres is observed using confocal laser microscopy. We characterize changes in diffusional characteristics of these molecules within alginate spheres as a function of incubation duration. We estimate diffusion coefficients (D) from fluorescence image series and observe a notable increase in capsule permeability once incubated in physiological media. Our strategy can serve as quantitative method to analyze structural changes in hydrogels.

Keywords

biomaterialbiomedicalpolymer
Type
Articles
Information
MRS Advances , Volume 3 , Issue 40: Soft Materials and Biomaterials , 2018 , pp. 2399 - 2408
Copyright
Copyright © Materials Research Society 2018 

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