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FITC Embedded ZnO/Silica Nanocomposites as probe for detection of L-lactate: Point-of-Care diagnosis

Published online by Cambridge University Press:  12 March 2019

S. S. Joglekar Open the ORCID record for S. S. Joglekar [Opens in a new window] ,
P. V. Pimpliskar ,
V. V. Sirdeshmukh ,
P. S. Alegaonkar  and
A. A. Kale Open the ORCID record for A. A. Kale [Opens in a new window]
S. S. Joglekar
Affiliation:
Department of Applied Physics, Defence Institute of Advanced technology, Pune-411025, India.
P. V. Pimpliskar
Affiliation:
Department of Applied Physics, Defence Institute of Advanced technology, Pune-411025, India.
V. V. Sirdeshmukh
Affiliation:
Applied Science Department, College of Engineering, Pune, Pune-411005, India.
P. S. Alegaonkar
Affiliation:
Department of Applied Physics, Defence Institute of Advanced technology, Pune-411025, India.
A. A. Kale*
Affiliation:
Applied Science Department, College of Engineering, Pune, Pune-411005, India.
*
*(Email: [email protected])
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Abstract:

A novel Fluorescence Resonance Energy Transfer (FRET) based ‘Turn-ON’ biosensor has been developed using fluorescent ZnO/APTMS-FITC (ZFA) nanoflakes as sensing probe. In this biosensor, Lactate Dehydrogenase (LDH) is used for the detection of L-lactate, a diagnostic marker for abnormal physiological conditions like muscular dystrophy, myocardial infraction, abnormal tissue formation and tissue damage. Lactate Dehydrogeanse (LDH) catalyses the conversion of L-Lactate to L-Pyruvate, in presence of β-NAD reducing to β-NADH. We tried to explore this mechanism with FRET based system for highly sensitive detection of L-Lactate. The fluorescence of these nanoflakes can be reversibly quenched in the presence of β-NAD.

Keywords

biomedicalcompositeluminescencenanoscaleoptical
Type
Articles
Information
MRS Advances , Volume 4 , Issue 46-47: Soft Materials and Biomaterials , 2019 , pp. 2533 - 2540
Copyright
Copyright © Materials Research Society 2019 

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References

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