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Image analysis for measuring lichen colonization on and within stonework

Published online by Cambridge University Press:  26 May 2009

Claudia GAZZANO ,
Sergio E. FAVERO-LONGO ,
Enrica MATTEUCCI  and
Rosanna PIERVITTORI
Claudia GAZZANO
Affiliation:
Department of Plant Biology and Centre of Excellence for Plant and Microbial Biosensing (CEBIOVEM), University of Torino, Viale Mattioli 25, 10125 Torino, Italy.
Sergio E. FAVERO-LONGO
Affiliation:
Department of Plant Biology and Centre of Excellence for Plant and Microbial Biosensing (CEBIOVEM), University of Torino, Viale Mattioli 25, 10125 Torino, Italy.
Enrica MATTEUCCI
Affiliation:
Department of Plant Biology and Centre of Excellence for Plant and Microbial Biosensing (CEBIOVEM), University of Torino, Viale Mattioli 25, 10125 Torino, Italy.
Rosanna PIERVITTORI*
Affiliation:
Department of Plant Biology and Centre of Excellence for Plant and Microbial Biosensing (CEBIOVEM), University of Torino, Viale Mattioli 25, 10125 Torino, Italy.
*
Email: [email protected]
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Abstract

The suitability of image analysis by colour-based pixel classification to quantify lichen colonization on the surface of and within marble, travertine and mortar stonework has been investigated. High resolution images of lichenized stonework surfaces were acquired at different field sites using a scanner, thus avoiding invasive surveys, and the percentage cover of lichen species was subsequently measured in the laboratory using dedicated software. Furthermore, microphotographs of polished cross-sections of lichenized marble, travertine and mortar, stained using the periodic acid-Schiff (PAS) method to visualize hyphae, were produced by the same software to quantify hyphal spread within the substratum, a parameter which can be used more successfully than the commonly used depth of hyphal penetration to quantify how much the lichen has affected the conservation of a stone substratum. Significant statistical differences in hue, saturation and intensity (HSI) of the lichen thalli and PAS-stained hyphae, with respect to the lithic substrata, allowed the software to discriminate and quantify the lichen species cover on, and hyphal spread within, the three investigated lithotypes. Since such a quantitative approach highlights the volume of influence of lichens on stonework, where bioweathering processes are likely to develop, it could be used to support decisions on the preservation of our stone cultural heritage.

Keywords

biodeteriorationcolour model HSIhyphal penetration componentlichen cover
Type
Research Article
Information
The Lichenologist , Volume 41 , Issue 3 , May 2009 , pp. 299 - 313
Copyright
Copyright © British Lichen Society 2009

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References

Adamo, P. & Violante, P. (2000) Weathering of rocks and neogenesis of minerals associated with lichen activity. Applied Clay Science 16: 229–256.Google Scholar
Ascaso, C., García del Cura, M. A. & de los Ríos, A. (2004) Microbial biofilms on carbonate rocks from a quarry and monuments in Novelda (Alicante, Spain). In Biodeterioration of Stone Surfaces (St. Clair, L. L. & Seaward, M. R. D., eds.): 79–98. Dordrecht: Kluwer Academic Publishers.Google Scholar
Banfield, J. F., Barker, W. W., Welch, S. A. & Taunton, A. (1999) Biological impact on mineral dissolution: Application of the lichen model to understanding mineral weathering in the rhizosphere. Proceedings of the National Academy of Sciences 96: 3404–3411.Google Scholar
Becerra, L., Soares, R. V., Bruno, L. S., Siqueira, C.C., Oppenheim, F. G., Offner, G. D. & Troxler, R. F. (2003) Patterns of secretion of mucins and non-mucin glycoproteins in human submandibular/sublingual secretion. Archives of Oral Biology 48: 147–154.CrossRefGoogle ScholarPubMed
Bjelland, T. & Ekman, S. (2005) Fungal diversity in rock beneath a crustose lichen as revealed by molecular markers. Microbial Ecology 49: 598–603.CrossRefGoogle ScholarPubMed
Chen, J., Blume, H. P. & Beyer, L. (2000) Weathering of rocks induced by lichen colonization – a review. Catena 39: 121–146.CrossRefGoogle Scholar
de los Ríos, A. & Ascaso, C. (2005) Contributions of in situ microscopy to the current understanding of stone biodeterioration. International Microbiology 8: 181–188.Google Scholar
Dietz, H. & Steinlein, T. (1996) Determination of plant species cover by means of image analysis. Journal of Vegetation Science 7: 131–136.CrossRefGoogle Scholar
Favero-Longo, S. E., Castelli, D., Salvadori, O., Belluso, E. & Piervittori, R. (2005) Pedogenetic action of the lichens Lecidea atrobrunnea, Rhizocarpon geographicum gr. and Sporastatia testudinea on serpentinized ultramafic rocks in an alpine environment. International Biodeterioration and Biodegradation 56: 17–27, 250–251.CrossRefGoogle Scholar
McCarthy, D. P. & Zaniewski, K. (2001) Digital analysis of lichen cover: a technique for use in lichenometry and lichenology. Arctic, Antarctic, and Alpine Research 31: 107–113.CrossRefGoogle Scholar
Nimis, P. L. (1991) Developments in lichen community studies. Lichenologist 23: 215–225.Google Scholar
Nimis, P. L., Monte, M. & Tretiach, M. (1987) Flora e vegetazione lichenica di aree archeologiche del Lazio. Studia Geobotanica 7: 3–161.Google Scholar
Nimis, P. L., Seaward, M. R. D., Ariño, X. & Barreno, E. (1998) Lichen-induced chromatic changes on monuments: a case-study on the Roman amphitheater of Italica (S. Spain). Plant Biosystems 132: 53–61.CrossRefGoogle Scholar
Nimis, P. L. & Martellos, S. (2008) ITALIC – The Information System on Italian Lichens. Version 4.0. University of Trieste, Dept. of Biology, IN4.0/1 (http://dbiodbs.univ.trieste.it/).Google Scholar
Piervittori, R., Salvadori, O. & Laccisaglia, A. (1994) Literature on lichens and biodeterioration of stonework. I. Lichenologist 26: 171–192.Google Scholar
Piervittori, R., Salvadori, O. & Laccisaglia, A. (1996) Literature on lichens and biodeterioration of stonework. II. Lichenologist 28: 471–483.Google Scholar
Piervittori, R., Salvadori, O. & Isocrono, D. (1998) Literature on lichens and biodeterioration of stonework. III. Lichenologist 30: 263–277.CrossRefGoogle Scholar
Piervittori, R., Salvadori, O., Isocrono, D. (2004 a) Literature on lichens and biodeterioration of stonework. IV. Lichenologist 36: 145–157.Google Scholar
Piervittori, R., Salvadori, O. & Seaward, M. R. D. (2004 b) Lichens and Monuments: an analytical bibliography. In Biodeterioration of Stone Surfaces (St. Clair, L. L. & Seaward, M. R. D., eds.): 241–282. Dordrecht: Kluwer Academic Publishers.Google Scholar
Pinna, D., Salvadori, O. & Tretiach, M. (1998) An anatomical investigation of calcicolous endolithic lichens from the Trieste karst (NE Italy). Plant Biosystems 132: 183–195.CrossRefGoogle Scholar
Purvis, O. W., Erotokritou, L., Wolseley, P. A., Williamson, B. & Read, H. (2002) A photographic quadrat recording method employing image analysis of lichens as indicator of environmental change. In Monitoring with Lichens – Monitoring Lichens (Nimis, P. L., Scheidegger, C. & Wolseley, P. A., eds.): 337–341. Dordrecht: Kluwer Academic Publishers.CrossRefGoogle Scholar
Roux, C. (1990) Étude écologique et phytosociologique des peuplements lichéniques saxicoles-calcicoles du Sud-Est de la France. Bibliotheca Lichenologica 15: 1–557.Google Scholar
Seaward, M. R. D. (2004) Lichens as subversive agents of biodeterioration. In Biodeterioration of Stone Surfaces (St. Clair, L. L. & Seaward, M. R. D., eds.): 9–18. Dordrecht: Kluwer Academic Publishers.CrossRefGoogle Scholar
Seefeldt, S. S. & Booth, D. T. (2006) Measuring plant cover in sagebrush steppe rangelands: a comparison of methods. Environmental Management 37: 703–711.CrossRefGoogle ScholarPubMed
St. Clair, L. L. & Seaward, M. R. D. (2004) Biodeterioration of rock substrata by lichens: progress and problems. In Biodeterioration of Stone Surfaces (St. Clair, L. L. & Seaward, M. R. D., eds.): 1–8. Dordrecht: Kluwer Academic Publishers.Google Scholar
Vicente-García, V., Ríos-Leal, E., Calderón-Domínguez, G., Cañizares-Villanueva, R. O., Olvera-Ramírez, R. (2004) Detection, isolation, and characterization of exopolysaccharide produced by a strain of Phormidium 94a isolated from an arid zone of Mexico. Biotechnology and Bioengineering 85: 306–310.Google Scholar
Vielhauer, V., Berning, E., Eis, V., Kretzler, M., Segerer, S., Strutz, F., Horuk, R., Gröne, H. J., Schlöndorff, D. & Anders, H. J. (2004) CCR1 blockade reduces interstitial inflammation and fibrosis in mice with glomerulosclerosis and nephrotic syndrome. Kidney International 66: 2264–2278.Google Scholar
Whitlach, R. B. & Johnson, R. G. (1974) Methods for staining organic matter in marine sediments. Journal of Sedimentary Petrology 44: 1310–1312.Google Scholar