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Species delimitation in the Lepraria isidiata-L. santosii group: a population study in the Mediterranean-Macaronesian region

Published online by Cambridge University Press:  08 January 2009

Mauro Tretiach ,
Lucia Muggia  and
Laurence Baruffo
Lucia Muggia
Affiliation:
Institut für Pflanzenwissenschaften, Karl-Franzens-Universität Graz, Holteigasse 6, A-8010 Graz, Austria.
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Abstract

A thorough chemical, molecular and morphological study has been carried out on a problematic group in the genus Lepraria characterized by lobed, granular thalli with a raised rim and a preference for mosses, rocks and mineral soils in exposed, xeric habitats. The material examined was collected in seven countries of the Mediterranean-Macaronesian region, from chalk, carbonate rocks and derived soils (A-thalli), and from siliceous rocks and derived soils (B-thalli). The results of a phylogenetic analysis based on 93 ITS sequences (29 of which newly obtained) support the identification and the segregation of two taxa at species level, provisionally identified as Lepraria isidiata s. lat., (most of the A-thalli), and L. santosii s. lat. (B-thalli plus the remaining A-thalli), as the observed genetic variability is quite high. Three chemotypes were detected in L. isidiata s. lat., and seven in L. santosii s. lat. The two taxa are morphologically well characterized: in L. isidiata s. lat. the thallus is thicker and the propagules larger than in L. santosii s. lat. The first stages of thallus development from single propagules are described in both species. Some critical remarks are made about the increasing use of the species rank for taxa of Lepraria, which are morphologically and genetically scarcely characterized.

Keywords

chemotypesITSmorphologyontogenyphylogeny
Type
Research Article
Information
The Lichenologist , Volume 41 , Issue 1 , January 2009 , pp. 1 - 15
Copyright
Copyright © British Lichen Society 2009

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References

REFERENCES

Baruffo, L., Zedda, L., Elix, J. A & Tretiach, M. (2006 a) A revision of the lichen genus Lepraria in Italy. Nova Hedwigia 83: 387–429.CrossRefGoogle Scholar
Baruffo, L., Muggia, L. & Tretiach, M. (2006 b) Posizione sistematica e distribuzione di due problematici taxa del genere Lepraria (Lecanorales, Ascomycota). Notizlario delta Società Lichenologica Italiona 19: 125.Google Scholar
Bayerová, S., Kukwa, M. & Fehrer, J. (2005) A new species of Lepraria (lichenized Ascomycetes) from Europe. Bryologist 108: 131–138.CrossRefGoogle Scholar
Casares-Porcel, M., Gonzalez-Tejero, M. R. & Bouchaalah, A. (1994) Contribución al conocimiento de la flora liquéoica gipsícola de Marruecos. Cryptogamie Bryologie-Lichénologie 15: 239–244.Google Scholar
Chaves, J. L., Lücking, R., Sipman, H. J. M., Umaña, L. & Navarro, E. (2004) A first assessment of the Ticolichen biodiversity inventory in Costa Rica: the genus Dyctionema (Polyporales: Atheliaceae). Bryologist 107: 242–249.CrossRefGoogle Scholar
Crespo, A., Argüello, A., Lumbsch, H. T., Llimona, X. & Tønsberg, T. (2006) A new species of Lepraria (Lecanorales: Stereocaulaceae) from the Canary Islands and the typification of Lepraria isidiata. Lichenologist 38: 213–221.CrossRefGoogle Scholar
Cubero, O. F., Crespo, A., Fatehi, J. & Bridge, P. D. (1999) DNA extraction and PCR amplification method suitable for fresh, herbarium stored and lichenized fungi. Plant Systematics and Evolution 217: 243–249.CrossRefGoogle Scholar
Ekman, S. & Tønsberg, T. (2002) Most species of Lepraria and Leproloma form a monophyletic group closely related to Stereocaulon. Mycological Research 106: 1262–1276.CrossRefGoogle Scholar
Gardes, M. & Bruns, T. D. (1993) ITS primers with enhanced specificity for basidiomycetes. Application for the identification of mycorrhizae and rust. Molecular Ecology 2: 113–118.CrossRefGoogle Scholar
Hall, T. A. (1999) BioEdit: a user friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acid Symposium Series 41: 95–98.Google Scholar
Holmgren, P. K., Holmgren, N. H. & Barnett, L. C. (1990) Index Herbariorum Part I: The Herbaria of the World. 8th edn. Regnum Vegetable 120. New York: New York Botanical Garden.Google Scholar
Huelsenbeck, J. P. & Ronquist, F. (2003) MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574.Google Scholar
Kukwa, M. (2002) Taxonomic notes on the lichen genera Lepraria and Leproloma. Annales Botanicae Fennici 39: 225–226.Google Scholar
Laundon, J. R. (1989) The species of Leproloma – the name for the Lepraria membranacea group. Lichenologist 21: 1–22.CrossRefGoogle Scholar
Laundon, J. R. (1992) Lepraria in the British Isles. Lichenologist 24: 315–350.CrossRefGoogle Scholar
Laundon, J. R. (2006) The subspecies of Melanelixia fuliginosa. Lichenologist 38: 277–278.CrossRefGoogle Scholar
Leuckert, C. & Kümmerling, H. (1991) Chemotaxonomische Studien in der Gattung Leproloma Nyl. ex Crombie (Lichenes). Nova Hedwigia 52: 17–32.Google Scholar
Leuckert, C., Kümmerling, H. & Wirth, V. (1995) Chemotaxonomy of Lepraria Ach. and Leproloma Nyl. ex Crombie with particular reference to Central Europe. Bibliotheca Lichenologica 58: 245–259.Google Scholar
Leuckert, C., Wirth, V., Kümmerling, H. & Heklau, M. (2004) Chemical lichen analyses XIV. Lepraria nivalis J. R. Laudon and Lepraria flavescens Cl. Roux & Tønsberg. Bibliotheca Lichenologica 88: 393–407.Google Scholar
Médail, F. & Quézel, P. (1997) Hot-spots analysis for conservation of plant biodiversity in the Mediterranean basin. Annals of the Missouri Botanical Garden 84: 112–127.CrossRefGoogle Scholar
Muggia, L., Grube, M. & Tretiach, M. (2008 a) A combined molecular and morphological approach to species delimitation in black-fruited, endolithic Caloplaca: high genetic and low morphological diversity. Mycological Research 112: 36–49.CrossRefGoogle ScholarPubMed
Muggia, L., Grube, M. & Tretiach, M. (2008 b) Genetic diversity and photobiont associations in selected taxa of the Tephromela atra group (Lecanorales, lichenized Ascomycota). Mycological Progress 7: 147–160.CrossRefGoogle Scholar
Nimis, P. L., Poelt, J. & Tretiach, M. (1996) Lichens from the Gypsum Park of the northern Apennines (N Italy). Cryptogamie Bryologie – Lichénologie 17: 23–38.Google Scholar
Orange, A. (1999) Lepraria Ach. and Leproloma Nyl. ex Crombie. In Lichen Atlas of the British Isles. Fascicle 4. Cavernularia, Degelia, Lepraria, Leproloma, Moelleropsis, Pannaria, Parmeliella (Seaward, M. R. D., ed.). London: British Lichen Society.Google Scholar
Orange, A. (2001) Lepraria atlantica, a new species from the British Isles. Lichenologist 33: 461–465.CrossRefGoogle Scholar
Orange, A., James, P. W. & White, F. J. (2001 a) Microchemical Methods for the Identification of Lichens. London: British Lichen Society.Google Scholar
Orange, A., Wolseley, P., Karunaratne, V. & Bombuwala, K. (2001 b) Two leprarioid lichens new to Sri Lanka. Bibliotheca Lichenologica 78: 327–333.Google Scholar
Page, R. D. M. (1996) TREEVIEW: An application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12: 357–358.Google ScholarPubMed
Poelt, J., Leuckert, C. & Roux, C. (1995) Die Arten der Lecanora dispersa-Gruppe (Lichenes, Lecanoraceae) auf kalkreichen Gesteinen im Bereich der Ostalpen – Eine Vorstudie. Bibliotheca Lichenologica 58: 289–333.Google Scholar
Posada, D. & Crandall, K. A. (1998) Modeltest – testing the model of DNA substitution. Bioinformatics 14: 817–818.CrossRefGoogle ScholarPubMed
Reid, W. V. (1998) Biodiversity hotspots. Trends in Ecology and Evolution 13: 275–280.CrossRefGoogle ScholarPubMed
Rodriguez, F., Oliver, J. L., Marin, A. & Medina, J. R. (1990) The general stochastic model of nucleotide substitution. Journal of Theoretical Biology 142: 485–501.CrossRefGoogle ScholarPubMed
Ronquist, F., Huelsenbeck, J. P. & Van der Mark, P. (2005) MrBayes 3.1 Manual.Google Scholar
Roux, C. (2003) Validigo de la taksonoj priskribitaj de J. Asta, G. Clauzade kaj C. Roux inter 1973 kaj 1977. Bulletin de la Société linnéenne de Provence 54: 119–123.Google Scholar
Saag, L. & Saag, A. (1999) The genus Lepraria (Lichenes imperfecti) in Estonia. Folia Cryptogamica Estonica 34: 55–63.Google Scholar
Saag, L., Saag, A. & Randlane, T. (2008) World survey of the genus Lepraria (Stereocaulaceae, lichenized Ascomycota). Lichenologist, in press.Google Scholar
Salvadori, O. & Tretiach, M. (2002) Thallus-substratum relationships of silicicolous lichens occurring on carbonatic rocks of the Mediterranean region. Bibliotheca Lichenologica 82: 57–64.Google Scholar
Sipman, H. J. M. (2004) Survey of Lepraria species with lobed thallus margins in the tropics. Herzogia 17: 23–35.Google Scholar
Slavíková-Bayerová, S. & Fehrer, J. (2007) New species of the Lepraria neglecta group (Stereocaulaceae, Ascomycota) from Europe. Lichenologist 39: 319–327.CrossRefGoogle Scholar
Slavíková-Bayerová, S. & Orange, A. (2006) Three new species of Lepraria (Ascomycota, Stereocaulaceae) containing fatty acids and atranorin. Lichenologist 38: 503–513.CrossRefGoogle Scholar
Timdal, E. (1992) A monograph of the genus Toninia (Lecideaceae, Ascomycetes). Opera Botanica 110: 1–137.Google Scholar
Tønsberg, T. (2004) Lepraria. In Lichen Flora of the Greater Sonoran Desert Region, Vol. 2 (Nash, T. H. III, Ryan, B. D., Diederich, P., Gries, C. & Bungartz, F., eds.): 322–329. Tempe (AZ): Arizona State University, Lichens Unlimited.Google Scholar
Vězda, A. (1973) Lichenes Selecti Exsiccati, Fasc. 47 (No. 1151–1175). Pruhonice prope Pragam: Institutum Botanicum Academiae Scientiarum Čechoslovacae.Google Scholar
White, F. J. & James, P. W. (1985) A new guide to microchemical techniques for the identification of lichen substances. British Lichen Society Bulletin 57 (supplement): 1–41.Google Scholar
White, T. J., Burns, T. D., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal DNA genes for phylogenies. In PCR Protocols, a Guide to Methods and Applications (Innis, M. A., Gelfand, D. H., Snisky, J. J. & T. J., White, eds): 315–322. San Diego: Academic Press.Google Scholar
Wirth, V., Düll, R., Llimona, X., Ros, R. M. & Werner, O. (2004) Guía de Campo de los Líquenes, Musgos y Hepáticas. Barcelona: Omega.Google Scholar