Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-25T16:42:05.067Z Has data issue: false hasContentIssue false
  • English
  • Français

Quinone reductase activity is widespread in lichens

Published online by Cambridge University Press:  26 May 2021

Calvin Eddington Moyo Open the ORCID record for Calvin Eddington Moyo [Opens in a new window] ,
Farida Minibayeva Open the ORCID record for Farida Minibayeva [Opens in a new window] ,
Christiane Liers  and
Richard Peter Beckett Open the ORCID record for Richard Peter Beckett [Opens in a new window]
Calvin Eddington Moyo
Affiliation:
School of Life Sciences, University of KwaZulu-Natal, Private Bag X01, Scottsville3209, South Africa
Farida Minibayeva
Affiliation:
Kazan Institute of Biochemistry and Biophysics, Federal Research Center ‘Kazan Scientific Center of RAS’, PO Box 261 Kazan420111, Russia
Christiane Liers
Affiliation:
Unit of Environmental Biotechnology, Dresden University of Technology, International Institute Zittau, Markt 23, 02763Zittau, Germany
Richard Peter Beckett*
Affiliation:
School of Life Sciences, University of KwaZulu-Natal, Private Bag X01, Scottsville3209, South Africa Open Lab ‘Biomarker’, Kazan (Volga Region) Federal University, Kremlevskaya str. 18, Kazan420008, Russia
*
Author for correspondence: Richard Peter Beckett. Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

In our earlier work, we demonstrated that the oxidases tyrosinase (TYR), laccase (LAC), and a heme peroxidase (POX) occur widely in lichens. Here we report on the occurrence of another oxidoreductase enzyme, quinone reductase (QR) (EC 1.6.5.5). While QR has been reported to occur widely in other organisms, there is currently no information on QR activities in lichens. Here we present a survey of QR activity in 14 species of lichens. Results demonstrate that QR activity is readily detectable in all lichen species tested. However, activities vary greatly, with ‘jelly’ lichens in the genera Collema and Leptogium having the highest activities. QR, LAC and POX are all believed to have a role in extracellular hydroxyl radical production. However, in this study no correlation was found between the activities of these enzymes and the rates at which hydroxyl radicals were produced. Possible roles for QR in lichen biology are discussed.

Keywords

detoxificationextracellular redox cyclinghydroxyl radicalsurvey
Type
Standard Papers
Information
The Lichenologist , Volume 53 , Issue 3 , May 2021 , pp. 265 - 269
Check for updates
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the British Lichen Society

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Akileswaran, L, Brock, BJ, Cereghino, JL and Gold, MH (1999) 1,4-benzoquinone reductase from Phanerochaete chrysosporium: cDNA cloning and regulation of expression. Applied and Environmental Microbiology 65, 415–421.CrossRefGoogle ScholarPubMed
Andlar, M, Rezic, T, Mardetko, N, Kracher, D, Ludwig, R and Santek, B (2018) Lignocellulose degradation: an overview of fungi and fungal enzymes involved in lignocellulose degradation. Engineering in Life Sciences 18, 768–778.CrossRefGoogle ScholarPubMed
Arantes, V and Goodell, B (2014) Current understanding of brown-rot fungal biodegradation mechanisms: a review. In Schultz, T (ed.), Deterioration and Protection of Sustainable Biomaterials. Washington, DC: American Chemical Society, pp. 3–21.CrossRefGoogle Scholar
Beckett, RP (1995) Some aspects of the water relations of lichens from habitats of contrasting water status studied using thermocouple psychrometry. Annals of Botany 76, 211–217.CrossRefGoogle Scholar
Beckett, RP, Zavarzina, AG and Liers, C (2013) Oxidoreductases and cellulases in lichens: possible role in lichen biology and soil organic matter turnover. Fungal Biology 117, 431–438.CrossRefGoogle ScholarPubMed
Beckett, RP, Minibayeva, FV, Vinogradova, A and Liers, C (2014) Hydration in the dark can induce laccase and peroxidase activity in Peltigeralean and non-Peltigeralean lichens. Lichenologist 46, 589–593.CrossRefGoogle Scholar
Beckett, RP, Ntombela, N, Scott, E, Gurjanov, OP, Minibayeva, FV and Liers, C (2015) Role of laccases and peroxidases in saprotrophic activities in the lichen Usnea undulata. Fungal Ecology 14, 71–78.CrossRefGoogle Scholar
Bissaro, B, Varnai, A, Rohr, AK and Eijsink, VGH (2018) Oxidoreductases and reactive oxygen species in conversion of lignocellulosic biomass. Microbiology and Molecular Biology Reviews 82, e00029.CrossRefGoogle ScholarPubMed
Bongard, RD, Krenz, GS, Gastonguay, AJ, Williams, CL, Lindemer, BJ and Merker, MP (2011) Characterization of the threshold for NAD(P)H: quinone oxidoreductase activity in intact sulforaphane-treated pulmonary arterial endothelial cells. Free Radical Biology and Medicine 50, 953–962.CrossRefGoogle ScholarPubMed
Brock, BJ and Gold, MH (1996) 1,4-benzoquinone reductase from the basidiomycete Phanerochaete chrysosporium: spectral and kinetic analysis. Archives of Biochemistry and Biophysics 331, 31–40.CrossRefGoogle ScholarPubMed
Brock, BJ, Rieble, S and Gold, MH (1995) Purification and characterization of a 1, 4-benzoquinone reductase from the basidiomycete Phanerochaete chrysosporium. Applied and Environmental Microbiology 61, 3076–3081.CrossRefGoogle ScholarPubMed
Cohen, R, Suzuki, MR and Hammel, KE (2004) Differential stress-induced regulation of two quinone reductases in the brown rot basidiomycete Gloeophyllum trabeum. Applied and Environmental Microbiology 70, 324–331.CrossRefGoogle ScholarPubMed
Devasagayam, TPA, Boloor, KK and Ramasarma, T (2003) Methods for estimating lipid peroxidation: an analysis of merits and demerits. Indian Journal of Biochemistry and Biophysics 40, 300–308.Google ScholarPubMed
Eggert, C, Temp, U and Eriksson, KE (1996) The ligninolytic system of the white rot fungus Pycnoporus cinnabarinus: purification and characterization of the laccase. Applied and Environmental Microbiology 62, 1151–1158.CrossRefGoogle ScholarPubMed
Espagne, E, Lespinet, O, Malagnac, F, Da Silva, C, Jaillon, O, Porcel, BM, Couloux, A, Aury, J-M, Ségurens, B, Poulain, J, et al. (2008) The genome sequence of the model ascomycete fungus Podospora anserina. Genome Biology 9, 1–22.CrossRefGoogle ScholarPubMed
Farrar, JF (1976) Ecological physiology of the lichen Hypogymnia physodes I. Some effects of constant water saturation. New Phytologist 77, 93–103.CrossRefGoogle Scholar
Gómez-Toribio, V, García-Martín, AB, Martínez, MJ, Martínez, ÁT and Guillén, F (2009 a) Enhancing the production of hydroxyl radicals by Pleurotus eryngii via quinone redox cycling for pollutant removal. Applied and Environmental Microbiology 75, 3954–3962.CrossRefGoogle ScholarPubMed
Gómez-Toribio, V, García-Martín, AB, Martínez, MJ, Martínez, ÁT and Guillén, F (2009 b) Induction of extracellular hydroxyl radical production by white-rot fungi through quinone redox cycling. Applied and Environmental Microbiology 75, 3944–3953.CrossRefGoogle ScholarPubMed
Leisner, JMR, Green, TGA and Lange, OL (1997) Photobiont activity of a temperate crustose lichen: long-term chlorophyll fluorescence and CO2 exchange measurements in the field. Symbiosis 23, 165–182.Google Scholar
Liers, C, Ullrich, R, Hofrichter, M, Minibayeva, FV and Beckett, RP (2011) A heme peroxidase of the ascomyceteous lichen Leptogium saturninum oxidizes high-redox potential substrates. Fungal Genetics and Biology 48, 1139–1145.CrossRefGoogle ScholarPubMed
Martinez, D, Challacombe, J, Morgenstern, I, Hibbett, D, Schmoll, M, Kubicek, CP, Ferreira, P, Ruiz-Duenas, FJ, Martinez, AT, Kersten, P, et al. (2009) Genome, transcriptome, and secretome analysis of wood decay fungus Postia placenta supports unique mechanisms of lignocellulose conversion. Proceedings of the National Academy of Sciences of the United States of America 106, 1954–1959.CrossRefGoogle ScholarPubMed
Moyo, CE, Beckett, RP, Trifonova, TV and Minibayeva, FV (2017) Extracellular redox cycling and hydroxyl radical production occurs widely in lichenized Ascomycetes. Fungal Biology 121, 582–588.CrossRefGoogle ScholarPubMed
Rao, PV, Krishna, CM and Zigler, JS (1992) Identification and characterization of the enzymatic activity of zeta-crystallin from guinea pig lens. A novel NADPH: quinone oxidoreductase. Journal of Biological Chemistry 267, 96–102.CrossRefGoogle Scholar