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Antibacterial, antifungal and cytotoxic effects of a sea cucumber Holothuria leucospilota, from the north coast of the Persian Gulf

Published online by Cambridge University Press:  26 February 2013

Flora Mohammadizadeh ,
Maryam Ehsanpor ,
Majid Afkhami ,
Amin Mokhlesi ,
Aida Khazaali  and
Shohreh Montazeri
Flora Mohammadizadeh*
Affiliation:
Islamic Azad University, Bandar Abbas Branch, PO Box: 79159-1311, Bandar Abbas, Iran
Maryam Ehsanpor
Affiliation:
Islamic Azad University, Bandar Abbas Branch, PO Box: 79159-1311, Bandar Abbas, Iran
Majid Afkhami
Affiliation:
Islamic Azad University, Bandar Abbas Branch, PO Box: 79159-1311, Bandar Abbas, Iran
Amin Mokhlesi
Affiliation:
Young Researchers Club, Tehran Central Branch, Islamic Azad University, Tehran, Iran
Aida Khazaali
Affiliation:
Islamic Azad University, Bandar Abbas Branch, PO Box: 79159-1311, Bandar Abbas, Iran
Shohreh Montazeri
Affiliation:
Islamic Azad University, Bandar Abbas Branch, PO Box: 79159-1311, Bandar Abbas, Iran
*
Correspondence should be addressed to: F. Mohammadizadeh, Islamic Azad University, Bandar Abbas Branch, PO Box: 79159-1311, Bandar Abbas, Iran email: [email protected]
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Abstract

Bioactive compounds of gonad, respiration tree, cuvierian organ, and body wall of the sea cucumber Holothuria leucospilota collected from the north coast of the Persian Gulf were extracted using ethyl acetate, methanol and water–methanol solvents. Extracts were evaluated for their antibacterial and antifungal activities against Aspergillus niger, Candida albicans, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. The activity was determined using the disc diffusion test. Cytotoxic activities of the extracts were determined by brine shrimp lethality assay. Results demonstrated that the A. niger was shown to be the most sensitive microorganism followed by C. albicans. The inhibition zone against A. niger and C. albicans had minimum inhibitory concentrations ranging from 3 to 7 μg/ml. The highest antifungal activity was found in G (water–methanol) with an inhibition zone of 50 mm against A. niger at 18 μg/ml extract concentration continuing with CT (methanol) with 46 mm inhibition zone against A. niger at 18 μg/ml extract concentration. The highest cytotoxic effect of methanol extract was found with LC50 values of about 40.31 μg/ml in the gonad organ from H. leucospilota continuing with the respiration tree organ with LC50 values of about 72.49 μg/ml.

Keywords

antibacterialantifungalcytotoxicHolothuria leucospilotaPersian Gulf
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 93 , Issue 5 , August 2013 , pp. 1401 - 1405
Copyright
Copyright © Marine Biological Association of the United Kingdom 2013 

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