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Novel 3D-printed methacrylated chitosan-laponite nanosilicate composite scaffolds enhance cell growth and biomineral formation in MC3T3 pre-osteoblasts

Published online by Cambridge University Press:  23 August 2018

Tugba Cebe ,
Neelam Ahuja Open the ORCID record for Neelam Ahuja [Opens in a new window] ,
Felipe Monte ,
Kamal Awad ,
Kimaya Vyavhare ,
Pranesh Aswath ,
Jian Huang ,
Marco Brotto  and
Venu Varanasi
Tugba Cebe
Affiliation:
Department of Materials Science and Engineering, University of Texas at Arlington, Arlington, Texas 76019, USA
Neelam Ahuja
Affiliation:
Department of Graduate Nursing, College of Nursing and Health Innovation, University of Texas at Arlington, Arlington, Texas 76019, USA
Felipe Monte
Affiliation:
Department of Graduate Nursing, College of Nursing and Health Innovation, University of Texas at Arlington, Arlington, Texas 76019, USA
Kamal Awad
Affiliation:
Department of Materials Science and Engineering, University of Texas at Arlington, Arlington, Texas 76019, USA; and Department of Refractories and Ceramics, National Research Centre, Giza 12622, Egypt
Kimaya Vyavhare
Affiliation:
Department of Materials Science and Engineering, University of Texas at Arlington, Arlington, Texas 76019, USA
Pranesh Aswath
Affiliation:
Department of Materials Science and Engineering, University of Texas at Arlington, Arlington, Texas 76019, USA
Jian Huang
Affiliation:
Department of Graduate Nursing, College of Nursing and Health Innovation, University of Texas at Arlington, Arlington, Texas 76019, USA
Marco Brotto
Affiliation:
Department of Graduate Nursing, College of Nursing and Health Innovation, University of Texas at Arlington, Arlington, Texas 76019, USA
Venu Varanasi*
Affiliation:
Department of Materials Science and Engineering, University of Texas at Arlington, Arlington, Texas 76019, USA; and Department of Graduate Nursing, College of Nursing and Health Innovation, University of Texas at Arlington, Arlington, Texas 76019, USA
*
a)Address all correspondence to this author. e-mail: [email protected]
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Abstract

This study compared the effect of gelatin- and chitosan-based scaffolds on osteoblast biomineralization. These scaffolds have been modified using methacrylate and laponite nanosilicates to improve their mechanical strength and support osteoblast function. Scaffold materials were prepared to have the same compressive strength (14–15 MPa) such that differences in cell response would be isolated to differences in biopolymer chemistry. The materials were tested for rheological properties to optimize the bio-ink for successful 3D printing using a robocast-assisted deposition system. Osteoblasts were cultured on the surface of 3D-printed methacrylated chitosan-laponite (MAC-Lp), methacrylated gelatin-laponite (MAG-Lp), MAC, and MAG scaffolds. MAC-Lp scaffolds showed increased cell viability, cell growth, and biomineral formation as compared to MAG-Lp scaffolds. FTIR results showed the presence of higher biomineral phosphate and extracellular matrix (ECM) collagen-like amide formation on MAC-Lp scaffolds as compared to MAG-Lp scaffolds. MAC-Lp scaffolds showed increased density of ECM-like tissue from SEM analysis, stained mineral nodules from Alizarin staining, and the existence of Ca–P species evident by X-ray absorbance near edge structure analysis. In conclusion, MAC-Lp scaffolds enhanced osteoblast growth and biomineral formation as compared to MAG-Lp scaffolds.

Keywords

biomaterialbone
Type
Article
Information
Journal of Materials Research , Volume 35 , Issue 1: Focus Section: Advances in Battery Technology: Material Innovations in Design and Fabrication , 14 January 2020 , pp. 58 - 75
Copyright
Copyright © Materials Research Society 2018 

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Since the original publication of this article, the Journal of Materials Research has moved to a new design layout. This article has been republished in the new design, and no changes have been made to the article content.

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