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Synthesis and characterization of citrus-derived pectin nanoparticles based on their degree of esterification

Published online by Cambridge University Press:  04 June 2020

Eden Mariam Jacob ,
Ankita Borah ,
Amandeep Jindal ,
Sindhu C. Pillai ,
Yohei Yamamoto ,
Toru Maekawa  and
Dasappan Nair Sakthi Kumar Open the ORCID record for Dasappan Nair Sakthi Kumar [Opens in a new window]
Eden Mariam Jacob
Affiliation:
Graduate School of Interdisciplinary New Science, Bio Nano Electronic Research Centre, Toyo University, Kawagoe, Saitama350-8585, Japan
Ankita Borah
Affiliation:
Graduate School of Interdisciplinary New Science, Bio Nano Electronic Research Centre, Toyo University, Kawagoe, Saitama350-8585, Japan
Amandeep Jindal
Affiliation:
Department of Materials Science, Faculty of Pure and Applied Sciences, University of Tsukuba, Tsukuba, Ibaraki305-8573, Japan
Sindhu C. Pillai
Affiliation:
Graduate School of Interdisciplinary New Science, Bio Nano Electronic Research Centre, Toyo University, Kawagoe, Saitama350-8585, Japan
Yohei Yamamoto
Affiliation:
Department of Materials Science, Faculty of Pure and Applied Sciences, University of Tsukuba, Tsukuba, Ibaraki305-8573, Japan
Toru Maekawa
Affiliation:
Graduate School of Interdisciplinary New Science, Bio Nano Electronic Research Centre, Toyo University, Kawagoe, Saitama350-8585, Japan
Dasappan Nair Sakthi Kumar*
Affiliation:
Graduate School of Interdisciplinary New Science, Bio Nano Electronic Research Centre, Toyo University, Kawagoe, Saitama350-8585, Japan
*
a)Address all correspondence to this author. e-mail: [email protected]
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Abstract

Polysaccharide-based nanoparticles such as pectin had always been of greatest interest because of its excellent solubility and mucoadhesive nature and are highly suitable for oral drug delivery for drug administration. In this study, we used commercially available pectin samples based on their degree of esterification, and nanoparticles were fabricated by the ionotropic gelation method using magnesium (Mg2+) as the divalent cross-linker. We conducted a comparative analysis on the three pectin NPs—high methoxylated pectin (HMP), low methoxylated pectin (LMP), and amidated LMP (AMP)—to examine the difference in characteristics such as shape, size, and biocompatibility. HMP and AMP were found to be similar in size (~850 nm), whereas LMP was found to be of ~700 nm. The three NPs were also tested for their biocompatibility toward THP-1 cells. All three NPs were found to have the potential as a nanocarrier of therapeutic and preventive drugs, especially through oral routes.

Keywords

pectin nanoparticlesionotropic gelationoral drug delivery
Type
Article
Information
Journal of Materials Research , Volume 35 , Issue 12 , 29 June 2020 , pp. 1514 - 1522
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Copyright © Materials Research Society 2020

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References

Salatin, S., Dizaj, S.M., and Khosroushahi, A.Y.: Effect of the surface modification, size, and shape on cellular uptake of nanoparticles. Cell Biol. Int. 39, 881–890 (2015).CrossRefGoogle ScholarPubMed
Nikalje, A.P.: Nanotechnology and its applications in medicine. Med. Chem. 5, 081–089 (2015).CrossRefGoogle Scholar
Peracchia, M.T., Gref, R., Minamitake, Y., Domb, A., Lotan, N., and Langer, R.: PEG-coated nanospheres from amphiphilic diblock and multiblock copolymers: Investigation of their drug encapsulation and release characteristics. J. Controlled Release 46, 223–231 (1997).CrossRefGoogle Scholar
May, C.D.: Industrial pectins: Sources, production, and applications. Carbohydr. Polym. 12, 79–99 (1990).10.1016/0144-8617(90)90105-2CrossRefGoogle Scholar
Sharma, R.K., Wooten, J.B., Baliga, V.L., and R Hajaligol, M.: Characterization of chars from biomass-derived materials: Pectin chars. Fuel 80, 1825–1836 (2001).10.1016/S0016-2361(01)00066-7CrossRefGoogle Scholar
Williats, W.G.T., Knox, P., and Mikkelsen, J.D.: Pectin: New insights into an old polymer. Trends Food Sci. Technol. 17, 97–104 (2006).10.1016/j.tifs.2005.10.008CrossRefGoogle Scholar
Valdés, A., Burgos, N., Jiménez, A., and Garrigós, M.C.: Natural pectin polysaccharides as edible coatings. Coatings 5, 865–886 (2015).10.3390/coatings5040865CrossRefGoogle Scholar
Noreen, A., Akram, J., Rasul, I., Mansha, A., Yaqoob, N., Iqbal, R., and Zia, K.M.: Pectins functionalized biomaterials; a new viable approach for biomedical applications: A review. Int. J. Biol. Macromol. 101, 254–272 (2017).10.1016/j.ijbiomac.2017.03.029CrossRefGoogle ScholarPubMed
Opanasopit, P., Apirakaramwong, A., Ngawhirunpat, T., Rojanarata, T., and Ruktanonchai, U.: Development and characterization of pectinate micro/nanoparticles for gene delivery. AAPS PharmSciTech 9, 67–74 (2008).10.1208/s12249-007-9007-7CrossRefGoogle ScholarPubMed
Jonassen, H., Treves, A., Kjøniksen, A.L., Smistad, G., and Hiorth, M.: Preparation of ionically cross-linked pectin nanoparticles in the presence of chlorides of divalent and monovalent cations. Biomacromolecules 14, 3523–3531 (2013).CrossRefGoogle ScholarPubMed
Monsoor, M.A., Kalapathy, U., and Proctor, A.: Improved method for determination of pectin degree of esterification by diffuse reflectance Fourier transform infrared spectroscopy. J. Agric. Food Chem., 49, 2756–2760 (2001).CrossRefGoogle ScholarPubMed
Kastner, H., Einhorn-Stoll, U., and Drusch, S.: Structure formation in sugar containing pectin gels-influence of gel composition and cooling rate on the gelation of non-amidated and amidated low-methoxylated pectin. Food Hydrocolloids 73, 13–20 (2017).10.1016/j.foodhyd.2017.06.023CrossRefGoogle Scholar
Mura, P., Maestrelli, F., Cirri, M., Luisa González Rodríguez, M., and Rabasco Alvarez, A.M.: Development of enteric-coated pectin-based matrix tablets for colonic delivery of theophylline. J. Drug Targeting 11, 365–371 (2003).10.1080/10611860310001639130CrossRefGoogle ScholarPubMed
Morris, G.A., Kök, S.M., Harding, S.E., and Adams, G.G.: Polysaccharide drug delivery systems based on pectin and chitosan. Biotechnol. Genet. Eng. Rev. 27, 257–284 (2010).CrossRefGoogle ScholarPubMed
Zhang, Y., Sun, T., and Jiang, C.: Biomacromolecules as carriers in drug delivery and tissue engineering. Acta Pharm. Sin. B 8, 34–50 (2018).10.1016/j.apsb.2017.11.005CrossRefGoogle ScholarPubMed
Birch, N.P. and Schiffman, J.D.: Characterization of self-assembled polyelectrolyte complex nanoparticles formed from chitosan and pectin. Langmuir 30, 3441–3447 (2014).CrossRefGoogle ScholarPubMed
Pourjavadi, A. and Barzegar, S.: Smart pectin‐based superabsorbent hydrogel as a matrix for ibuprofen as an oral non‐steroidal anti‐inflammatory drug delivery. Starch‐Stärke 61, 173–187 (2009).10.1002/star.200800032CrossRefGoogle Scholar
Subudhi, M.B., Jain, A., Jain, A., Hurkat, P., Shilpi, S., Gulbake, A., and Jain, S.: Eudragit S100 coated citrus pectin nanoparticles for colon targeting of 5-fluorouracil. Materials 8, 832–849 (2015).CrossRefGoogle ScholarPubMed
Al Alawi, A.M., Majoni, S.W., and Falhammar, H.: Magnesium and human health perspectives and research directions. Int. J. Endocrinol. 2018, 1–17 (2018).CrossRefGoogle ScholarPubMed
Gadalla, H.H., El-Gibaly, I., Soliman, G.M., Mohamed, F.A., and El-Sayed, A.M.: Amidated pectin/sodium carboxymethylcellulose microspheres as a new carrier for colonic drug targeting: Development and optimization by factorial design. Carbohydr. Polym., 153, 526–534 (2016).10.1016/j.carbpol.2016.08.018CrossRefGoogle ScholarPubMed
Vauthier, C. and Bouchemal, K.: Methods for the preparation and manufacture of polymeric nanoparticles. Pharmaceut. Res. 26, 1025–1058 (2009).CrossRefGoogle ScholarPubMed
Jones, O.G. and McClements, D.J.: Recent progress in biopolymer nanoparticle and microparticle formation by heat-treating electrostatic protein–polysaccharide complexes. Adv. Colloid Interface Sci. 167, 49–62 (2009).10.1016/j.cis.2010.10.006CrossRefGoogle Scholar
Yan, J.K., Qiu, W.Y., Wang, Y.Y., and Wu, J.Y.: Biocompatible polyelectrolyte complex nanoparticles from lactoferrin and pectin as potential vehicles for antioxidative curcumin. J. Agric. Food Chem. 65, 5720–5730 (2017).CrossRefGoogle ScholarPubMed
Chittasupho, C., Jaturanpinyo, M., and Mangmool, S.: Pectin nanoparticle enhances cytotoxicity of methotrexate against hepG2 cells. Drug Deliv. 20, 1–9 (2013).CrossRefGoogle ScholarPubMed
Andishmand, H., Tabibiazar, M., Mohammadifar, M.A., and Hamishehkar, H.: Pectin-zinc-chitosan-polyethylene glycol colloidal nano-suspension as a food grade carrier for colon targeted delivery of resveratrol. Int. J. Biol. Macromol. 97, 16–22 (2017).CrossRefGoogle ScholarPubMed
Burapapadh, K., Takeuchi, H., and Sriamornsak, P.: Development of pectin nanoparticles through mechanical homogenization for dissolution enhancement of itraconazole. Asian J. Pharm. Sci. 11, 365–375 (2016).10.1016/j.ajps.2015.07.003CrossRefGoogle Scholar
Sinitsya, A., Čopíková, J., Prutyanov, V., Skoblya, S., and Machovič, V.: Amidation of highly methoxylated citrus pectin with primary amines. Carbohydr. Polym. 42, 359–368 (2000).CrossRefGoogle Scholar
Jeevanandam, J., San Chan, Y., and K Danquah, M.: Biosynthesis and characterization of MgO nanoparticles from plant extracts via induced molecular nucleation. New J. Chem. 41, 2800–2814 (2000).CrossRefGoogle Scholar
Zhang, W. and Zhou, Z.: Citrus pectin-derived carbon microspheres with superior adsorption ability for methylene blue. Nanomaterials 7, 161 (2017).10.3390/nano7070161CrossRefGoogle ScholarPubMed
Hussien, N.A., Işıklan, N., and Türk, M.: Pectin-conjugated magnetic graphene oxide nanohybrid as a novel drug carrier for paclitaxel delivery. Artif. Cells, Nanomed., Biotechnol. 46, 264–273 (2018).10.1080/21691401.2017.1421211CrossRefGoogle ScholarPubMed
Markov, P.A., Krachkovsky, N.S., Durnev, E.A., Martinson, E.A., Litvinets, S.G., and Popov, S.V.: Mechanical properties, structure, bioadhesion, and biocompatibility of pectin hydrogels. J. Biomed. Mater. Res., Part A 105, 2572–2581 (2017).CrossRefGoogle ScholarPubMed
Li, C., Nie, H., Chen, Y., Xiang, Z.Y., and Li, J.B.: Amide pectin: A carrier material for colon‐targeted controlled drug release. J. Appl. Polym. Sci. 133 (2016).10.1002/app.43697CrossRefGoogle Scholar
Thirawong, N., Nunthanid, J., Puttipipatkhachorn, S., and Sriamornsak, P.: Mucoadhesive properties of various pectins on gastrointestinal mucosa: An in vitro evaluation using texture analyzer. Eur. J. Pharm. Biopharm. 67, 132–140 (2007).CrossRefGoogle Scholar
Oakenfull, D.G.: The chemistry of high-methoxyl pectins. In The Chemistry and Technology of Pectin, R. Walters and S. Taylor, eds. (Academic Press, New York, 1991); pp. 87–108.Google Scholar
Sriamornsak, P.: Chemistry of pectin and its pharmaceutical uses: A review. Silpakorn Univ. Int. J. 3, 206–228 (2003).Google Scholar
Topuz, F., Henke, A., Richtering, W., and Groll, J.: Magnesium ions and alginate do form hydrogels: A rheological study. Soft Matter 8, 4877 (2012).CrossRefGoogle Scholar
Powell, D.A., Morris, E.R., Gidley, M.J., and Rees, D.A.: Conformations and interactions of pectins: II. Influence of residue sequence on chain association in calcium pectate gels. J. Mol. Biol. 155, 517–531 (1982).10.1016/0022-2836(82)90485-5CrossRefGoogle Scholar
Endress, H.U. and Mattes, F.: Pectin. In Fiber Ingredients S.S. Chou and P. Samuel, eds. (CRC Press, Boca Ranton, Florida, 2009); pp. 149–186.Google Scholar
Thakur, B.R., Singh, R.K., Handa, A.K., and Rao, M.A.: Chemistry and uses of pectin: A review. Crit. Rev. Food Sci. Nutr. 37, 47–73 (1997).10.1080/10408399709527767CrossRefGoogle ScholarPubMed
Wehr, J.B., Menzies, N.W., and Blamey, F.P.C.: Alkali hydroxide-induced gelation of pectin. Food Hydrocolloids 18, 375–378 (2004).CrossRefGoogle Scholar
Yoo, S.H., Lee, B.H., Savary, B.J., Lee, S., Lee, H.G., and Hotchkiss, A.T.: Characteristics of enzymatically-deesterified pectin gels produced in the presence of monovalent ionic salts. Food Hydrocolloids 23, 1926–1929 (2009).CrossRefGoogle Scholar
Reena, K., Prabakaran, M., Leeba, B., Gajendiran, M., and Antony, S.A.: Green synthesis of pectin-gold-PLA-PEG-PLA nanoconjugates: In vitro cytotoxicity and anti-inflammatory activity. J. Nanosci. Nanotechnol. 17, 4549–4557 (2017).CrossRefGoogle Scholar
Lin, L., Xu, W., Liang, H., He, L., Liu, S., Li, Y., and Chen, Y.: Construction of pH-sensitive lysozyme/pectin nanogel for tumor methotrexate delivery. Colloids Surf. B Biointerfaces 126, 459–466 (2015).CrossRefGoogle ScholarPubMed
Gnanasambandam, R. and Proctor, A.: Determination of pectin degree of esterification by diffuse reflectance Fourier transform infrared spectroscopy. Food Chem. 68, 327–332 (2000).CrossRefGoogle Scholar
Jaiswal, S.S.S., Duffy, B., and Jaiswal, A.K.: Nanostructured materials for food applications: Spectroscopy, microscopy and physical properties. Bioengineering 6, 26 (2019).Google Scholar
Dobrovolsky, V.D., Khyzhun, O.Y., Sinelnichenko, A.K., Ershova, O.G., and Solonin, Y.M.: XPS study of influence of exposure to air on thermal stability and kinetics of hydrogen decomposition of MgH2 films obtained by direct hydrogenation from gaseous phase of metallic Mg. J. Electron Spectrosc. Relat. Phenom. 215, 28–35 (2017).10.1016/j.elspec.2017.01.001CrossRefGoogle Scholar
Pistone, S., Goycoolea, F.M., Young, A., Smistad, G., and Hiorth, M.: Formulation of polysaccharide-based nanoparticles for local administration into the oral cavity. Eur. J. Pharm. Sci. 96, 381–389 (2017).CrossRefGoogle ScholarPubMed
Aldo, P.B., Craveiro, V., Guller, S., and Mor, G.: Effect of culture conditions on the phenotype of THP‐1 monocyte cell line. Am. J. Reprod. Immunol. 70, 80–86 (2013).CrossRefGoogle ScholarPubMed
Borker, S. and Pokharkar, V.: Engineering of pectin-capped gold nanoparticles for delivery of doxorubicin to hepatocarcinoma cells: An insight into mechanism of cellular uptake. Artif. Cells, Nanomed., Biotechnol. 46, 826–835 (2018).10.1080/21691401.2018.1470525CrossRefGoogle ScholarPubMed
Cui, S., Yao, B., Gao, M., Sun, X., Gou, D., Hu, J., and Liu, Y.: Effects of pectin structure and crosslinking method on the properties of crosslinked pectin nanofibers. Carbohydr. Polym. 157, 766–774 (2017).CrossRefGoogle ScholarPubMed
Akram, M.W., Fakhar-e-Alam, M., Atif, M., Butt, A.R., Asghar, A., Jamil, Y., and Wang, Z.M.: In vitro evaluation of the toxic effects of MgO nanostructure in Hela cell line. Sci. Rep. 8, 4576 (2018).CrossRefGoogle ScholarPubMed
Sriamornsak, P.: Application of pectin in oral drug delivery. Expet Opin. Drug Deliv. 8, 1009–1023 (2011).10.1517/17425247.2011.584867CrossRefGoogle ScholarPubMed