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Expression of substance P, vasoactive intestinal peptide and heat shock protein 70 in nasal mucosal smears of patients with allergic rhinitis: investigation using a liquid-based method

Published online by Cambridge University Press:  19 February 2008

T Chalastras ,
P Nicolopoulou-Stamati ,
E Patsouris ,
A Eleftheriadou ,
D Kandiloros ,
I Yiotakis ,
M Gonidi  and
P Athanassiadou
T Chalastras*
Affiliation:
Department of Pathology Medical School, University of Athens, Athens, Greece
P Nicolopoulou-Stamati
Affiliation:
Department of Pathology Medical School, University of Athens, Athens, Greece
E Patsouris
Affiliation:
Department of Pathology Medical School, University of Athens, Athens, Greece
A Eleftheriadou
Affiliation:
Department of Otolaryngology, Medical School, University of Athens, Hippokration Hospital, Athens, Greece
D Kandiloros
Affiliation:
Department of Otolaryngology, Medical School, University of Athens, Hippokration Hospital, Athens, Greece
I Yiotakis
Affiliation:
Department of Otolaryngology, Medical School, University of Athens, Hippokration Hospital, Athens, Greece
M Gonidi
Affiliation:
Department of Pathology Medical School, University of Athens, Athens, Greece
P Athanassiadou
Affiliation:
Department of Pathology Medical School, University of Athens, Athens, Greece
*
Address for correspondence: Dr Thomas Chalastras, Tenarou 35, Glyfada-Athens, GreecePC 16562. Fax: +302107223033 E-mail: [email protected]
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Abstract

Objective:

The aim of this study was to investigate expression of the neuropeptides substance P, vasoactive intestinal peptide and heat shock protein 70 in the nasal mucosa cells of patients with seasonal allergic rhinitis, in order to obtain more information on the pathophysiological and immunological role of these markers in allergic rhinitis.

Material and methods:

Nasal epithelium specimens obtained from 42 patients with allergic rhinitis were studied, using Shandon's Papspin liquid-based cytology method. Smears were immunostained with antibodies against substance P, vasoactive intestinal peptide and heat shock protein 70, and the results were correlated with the clinical features of seasonal allergic rhinitis.

Results:

A positive reaction for substance P, vasoactive intestinal peptide and heat shock protein 70 was observed in 73.8, 66.7 and 69.0 per cent of the allergic rhinitis mucosal smears, respectively. The Pearson chi-square test showed that 40.5 per cent of the immunostained smears had a positive reaction for one or two of the markers studied (i.e. substance P, vasoactive intestinal peptide or heat shock protein 70), and that 47.6 per cent of the smears had a positive reaction for all the markers (p < 0.0001).

Conclusions:

We found a high level of expression of substance P and vasoactive intestinal peptide in the nasal mucosa smears of patients suffering from allergic rhinitis. This indicates a role for these neuropeptides in the neuroregulation of immunity and hypersensivity in this disease. Furthermore, expression of heat shock protein 70 may contribute to the development of allergic rhinitis.

Keywords

Seasonal Allergic RhinitisNeuropeptidesVIP ReceptorsSubstance P
Type
Main Article
Information
The Journal of Laryngology & Otology , Volume 122 , Issue 7 , July 2008 , pp. 700 - 706
Copyright
Copyright © JLO (1984) Limited 2008

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References

1 Parikh, A, Scadding, G. Fortnightly review: seasonal allergic rhinitis. BMJ 1997;314:1392–5CrossRefGoogle Scholar
2 Quraishi, S, Davies, M, Craig, T. Inflammatory responses in allergic rhinitis: traditional approaches and novel treatment strategies. J AM Osteopath Assos 2004;104(Suppl 5):S7–15Google ScholarPubMed
3 White, M. Mediators of inflammation and the inflammatory process. J Allergy Clin Immunol 1999;103 Supplement March 1999:S378–81CrossRefGoogle ScholarPubMed
4 Naclerio, R. Clinical manifestations of the release of histamine and other inflammatory mediators. J Allergy Clin Immunol 1999;103 Supplement March 1999:S382–5CrossRefGoogle ScholarPubMed
5 American Academy of Allergy, Asthma and Immunology. The Allergy Report. Overview of Allergic Diseases: Diagnosis, Management and Barriers to Care. Milwaukee: American Academy of Allergy, Asthma, and Immunology, 2004;1:1–97Google Scholar
6 Varga, E, Jacobson, M, Masuyama, K, Rak, S, Till, S, Darby, Y et al. Inflammatory cell populations and cytokine mRNA expression in the nasal mucosa in aspirin-sensitive rhinitis. Eur Resp J 1999;14:610–15Google Scholar
7 Pipkorn, U. Mediators and nasal allergy. Clin Exp Allergy 1989;19:585–9CrossRefGoogle ScholarPubMed
8 Heppt, W, Thai Dihn, Q, Cryer, A, Zweng, M, Noga, O, Peiser, C et al. Phenotypic alteration of neuropeptide-containing nerve fibres in seasonal intermittent allergic rhinitis. Clin Exp Allergy 2004;34:1105–10CrossRefGoogle ScholarPubMed
9 Fang, S, Shen, C. Neuropeptide inervation and neuroendocrine cells in allergic rhinitis and chronic hypertrophic rhinitis. Clin Exp Allergy 1997;28:228–32CrossRefGoogle Scholar
10 Fischer, A, Wussow, A, Cryer, A, Schmeck, B, Noga, O, Zweng, M et al. Neuronal plasticity in persistent perennial allergic rhinitis. J Occup Env Med 2005;47:20–5Google Scholar
11 Baraniuk, J, Lundgren, J, Okayama, M, Mullolo, J, Merida, M, Shelhamer, J et al. Vasoactive intestinal peptide in human nasal mucosa. J Clin Invest 1990;86:825–31CrossRefGoogle ScholarPubMed
12 Larson, O, Duner-Engstrom, M, Lundberg, J, Freholm, B, Anggard, A. Effects of VIP, PHM and substance P on blood vessels and secretory elements of the human submandibular gland. Regul Pept 1986;13:319–26Google Scholar
13 Malm, L, Sundler, F, Uddman, R. Effects of vasoactive intestinal peptide (VIP) on resistance and capacitance vessels in nasal mucosa. Acta Otolaryngol (Stock) 1980;90:304–8CrossRefGoogle ScholarPubMed
14 Fajac, I, Frossard, N. Neuropeptides of the nasal innervation and allergic rhinitis [in French]. Rev Mal Resp 1994;11:357–67Google Scholar
15 Myers, A, Kajekar, R, Undem, B. Allergic inflammation-induced neuropeptide production in rapidly adapting afferent nerves in guinea pig airways. Am J Physiol 2002;282:L755–81Google ScholarPubMed
16 Jin, G, Nakayama, H, Shmyhlo, M, Inoue, S, Kondo, M, Ikezawa, Z et al. High positive frequency of antibodies to metallothionein and heat shock protein 70 in sera of patients with metal allergy. Clin Exp Immunol 2003;131:275–9Google Scholar
17 Aron, Y, Busson, M, Polla, B, Dusser, D, Lockhart, A, Swierczewski, E et al. Analysis of Hsp 70 gene polymorphism in allergic asthma. Allergy 1999;54:165–70Google Scholar
18 Liu, L, Xiao, C, Zhang, M, Cheng, L, Wang, E, Wu, T. Expression of Hsp in peripheral lymphocyte of the patients with allergic rhinitis. J Huazhong Univ Sci Technolog Med Sci 2003;23:310–12Google Scholar
19 Cordell, J, Falini, B, Erber, W, Ghosh, A, Abdulaziz, Z, MacDonald, S. Immuno-enzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase APAAP complexes. J Histochem Cytochem 1984;32:219–29CrossRefGoogle Scholar
20 Mosimman, B, White, M, Hohman, R. Substance P, calcitonin gene-related peptide, and vasoactive intestinal peptide increase in nasal secretions after allergen challenge in atopic patients. J Allergy Clin Immunol 1993;92:95–104CrossRefGoogle Scholar
21 Rayan, D. Neuropeptides. West J Med 1987;146:81–2Google Scholar
22 Lambrecht, B. Immunologists getting nervous: neuropeptides, dentritic cells and T cell activation. Respir Res 2001;2:133–8Google Scholar
23 Kaltreider, H, Ichikawa, S, Byrd, P, Ingram, D, Kishiyama, J, Sreedharan, S et al. Upregulation of neuropeptides and neuropeptide receptors in a murine model of immune inflammation in lung parenchyma. Am J Respir Cell Mol Biol 1997;16:133–44Google Scholar
24 Reubi, J, Horisberger, U, Kappeler, A, Laissue, J. Localization of receptors for vasoactive intestinal peptide, somatostatin and substance P in distinct compartments of human lymphoid organs. Blood 1998;92:191–7CrossRefGoogle ScholarPubMed
25 Lundberg, J, Anggard, A, Fahrenbrug, J. Co-release of VIP and acetylcholine in relation to blood flow in salivary secretion in cat submandibular gland. Acta Physiol Scand 1982;115:525–8Google Scholar
26 Baraniuk, J, Kaliner, M. Neuropeptides and nasal secretion. Am J Physiol 1991;261:223–35Google ScholarPubMed
27 Payan, D, Goetzl, E. Modulation of lymphocyte function by sensory neuropeptides. J Immunol 1985;135:783–6Google Scholar
28 Kurian, S, Blank, A, Shepard, M. Vasoactive intestinal polypeptide (VIP) in vasomotor rhinitis. Clin Biochem 1983;11:425–6Google Scholar
29 Kaufmann, S. Heat shock proteins and the immune response. Immunol Today 1990;11:129–36Google Scholar
30 Schlesinger, M. Heat shock proteins. J Biol Chem 1990;59:591–601Google Scholar
31 Kindas-Mugge, I, Steiner, G, Smolen, J. Similar frequency of autoantibodies against 70-kD class heat shock proteins in healthy subjects and systemic lupus erythematosus patients. Clin Exp Immunol 1993;92:46–50CrossRefGoogle ScholarPubMed
32 Weynand, B, Berliene, M, Haumont, E, Massart, F, Pourvoyeur, A, Bernard, P et al. A new, liquid-based cytology technique. Acta Cytol 2003;47:149–53CrossRefGoogle ScholarPubMed