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Carbohydrate content of the endolymphatic sac. A histochemical and lectin-labelling study in the mongolian gerbil

Published online by Cambridge University Press:  29 June 2007

M. Barbara
M. Barbara*
Affiliation:
Department of Otolaryngology, University ‘La Sapienza’, Rome, Italy Department of Otolaryngology, Karolinska Hospital and Karolinska Institute, Stockholm, Sweden
*
M. Barbara, Department Otolaryngology, University ‘La Sapienza’, 00185 Rome, Italy
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Abstract

A secretory activity has recently been attributed to the endolymphatic sac (ES), as a possible way to contribute to the fluid balance of the entire endolymphatic compartment. Previous histochemical studies have indicated the existence of carbohydrate complexes in the secretory product, both neutral and acidic in nature.

A thorough analysis of these compounds in the gerbilline ES was carried out using both transmission electron microscopic (TEM) histochemistry, using dialyzed iron and periodic acid—chromic—silver staining techniques, as well as immunoflourescence with fluorescein-labelled lectins (FITC-lectins).

N-acetylglucosamine was found to be one of the major carbodhydrate components both of the epithelial layer of the ES and of the luminal precipitate. Proofs for a local secretory activity and its intracellular pathway are presented, suggesting to be involved in the regulation of pressure and volume of inner ear fluids.

Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 103 , Issue 2 , February 1989 , pp. 137 - 142
Copyright
Copyright © JLO (1984) Limited 1989

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