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Nuclear DNA content using computerized image cytometry of squamous cell carcinomas of the head and neck

Published online by Cambridge University Press:  29 June 2007

S. K. Sarker ,
K. Ghufoor ,
K. S. Patel ,
N. S. Tolley  and
D. V. Coleman
S. K. Sarker*
Affiliation:
Cytopathology, Cytogenetics, Units, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, University of London, London, UK. Academic Surgical, Units, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, University of London, London, UK Head and Neck, Units, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, University of London, London, UK.
K. Ghufoor
Affiliation:
Head and Neck, Units, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, University of London, London, UK.
K. S. Patel
Affiliation:
Head and Neck, Units, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, University of London, London, UK.
N. S. Tolley
Affiliation:
Head and Neck, Units, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, University of London, London, UK.
D. V. Coleman
Affiliation:
Cytopathology, Cytogenetics, Units, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, University of London, London, UK.
*
Address for correspondence: S. K. Sarker, Cytopathology and Cytogenetics Unit, Clarence Wing, St Mary's Hospital Medical School, Praed Street, London W2 1NY. Fax: 0171-725-1262
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Abstract

Ploidy status using flow cytometry of head and neck cancers may be of prognostic value. We describe the use of image cytometry in ploidy measurement of squamous cell carcinomas of the head and neck (SCCHN). This technique allows only tumour cells to be measured, thereby rejecting debris, artefact and benign cells. Tissue sections were cut from tumours and then Feulgen stained. A total of 60 patients were included in this study, 23 females and 37 males. The data reveals a relationship between ploidy status and the histological differentiation. However, the ploidy status and histological differentiation do not appear to correlate to the clinical stage of the disease. This method of measuring ploidy may be more accurate than flow cytometry and may have a prognostic role in head and neck cancer patients. A study comparing both methods may demonstrate this and we aim to evaluate this in the future.

Keywords

DNAImage cytometryHead and neck neoplasmsCarcinomasquamous cell
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 111 , Issue 1 , January 1997 , pp. 43 - 47
Copyright
Copyright © JLO (1984) Limited 1997

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